ABSTRACT
Objective: To investigate the chemical constituents from Rhamnus heterophylla. Methods: Multiple chromatographic separation techniques including silica gel, Sephadex LH-20 gel and MCI gel were employed to isolate and purify the compounds. Their structures were identified by means of the nuclear magnetic resonance (NMR) and physicochemical properties. The chemical compositions were quickly analyzed by HPLC-DAD-ESI-MS/MS and scanned in negative ions. Results: These compounds were isolated and determined as cis-aloeemodin bianthrone (1), trans-aloeemodin bianthrone (2), emodin-8-O-rhamnoside (3), citreorosein (4), emodin (5), chrysophanol (6), physcion (7), kaempferol (8), luteolin (9), and quercetin (10). In addition to compounds 5, 8, the other compounds are isolated from R. heterophylla for the first time, and the compound 3 is isolated from the genus Rhamnus for the first time. 28 Compounds were speculated via comparing MS data (detected mass and MS2 fragment ions) and coupled with related literature, including three hydroxybenzoic acids, eight anthraquinones and 17 flavonoids. Conclusion: The analysis of chemical constituents from R. heterophylla could provide the references for the study on pharmacodynamic material basis and reasonable application and development.