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ObjectiveTo discuss the expression of tumor shift suppressor gene KAI1,matrix metal proteinase 9 (MMP-9) in endometriosis sickness in dystopia internal membrane and its significance.MethodsIn 53 patients and 30 healthy controls,the expression of KAI1,MMP-9 in the endometriosis was detected with immunohistochemistry SP method,and the relationship was analyzed.ResultsKAI1 had low expression in EMS patients,while MMP-9 was highly expressed.In the control group,KAI1 was highly expressed while MMP-9 lowly expressed,and the differences between two groups were remarkably significant (P ＜0.05).KAI1 and the MMP-9 expression showed negative correlation between two groups.Conclusions KAI1 and MMP-9 was negatively expressed in EMS,which may play a role in the EMS prognosis.KAI1 and MMP-9 may be served as the prognosis and monitor markers for EMS.
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Objective To investigate the.expression and significance of peritoneal fluid (PF) erythropoietin (EPO) and neutrophils activation peptide-78 (ENA-78)in patients with endometriosis.Methods The levels of EPO and ENA-78 in peritoneal fluid (PF) were measured by means of enzymelinked immunosobent assay (ELISA) in 30 women with endometriosis (9 in Ⅰ ～ Ⅱ stage,21 in Ⅲ～ Ⅳ stage)and 30 without endometriosis.And the relationship between the PF levels of EPO and ENA-78 in patients with endometriosis was analyzed.Results The PF levels of EPO and ENA-78 in women with endometriosis [ (9.272 ± 2.284 ) IU/L,( 2.068 ± 0.794 ) ng/ml ] were significantly higher than those in control group [ (5.759 ±0.502) IU/L,(0.886 ±0.145 ) ng/ml,P ＜0.05].There were no significant differences in the PF levels of EPO between stage Ⅰ ～ Ⅱ [ (9.549 ± 1.996) IU/L] and Ⅲ ～ Ⅳ [ (9.181 ± 1.897) IU/L,P ＞0.05].The ENA-78 concentrations of PF in stage Ⅲ～ Ⅳ [ (2.517 ± 0.518 )ng/ml] were significantly higher than those in stage Ⅰ ～ Ⅱ[ ( 1.137 ±0.169)ng/ml,P ＜0.05].There were no obvious correlations in PF EPO and ENA-78 in patients with endometriosis.Conclusions The excessive expression of EPO and ENA-78 in the PF of women with endometriosis suggests that EPO and ENA-78 may be an important role in the pathogenesis of endometriosis.
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Objective To explore the changes of GRO-α and VEGF in the peritoneal fluid of women with endometriosis and their correlation. Methods The levels of GRO-α and VEGF were detected by enzyme linked immunosorbent assay ( ELISA ) method in 39 cases of endometriosis and 25 normal cases of without endometriosis. Results The levels of GRO-αand VEGF in the peritoneal fluid of EMs group were ( 108.77 ± 20. 58 ) pg/ml and ( 199. 67 ± 99. 26 ) pg/ml separately, and it was significantly higher than those in control group ( 71.07 ± 18. 96 ) pg/ml, ( 115.46 ± 32. 39 ) pg/ml. The differences were statically significant( P <0. 05). The level of GRO-α in the peritoneal fluid of the patients in stage Ⅰ and Ⅱ was sig-nificantly lower than that in stage Ⅲ and Ⅳ[(99.43 ± 16.05)pg/ml vs (117.65 ± 20.8)pg/ml, P <0. 05] , while the level of VEGF in the peritoneal fluid of the patients in stage Ⅰ and Ⅱ was significantly higher than that in stage Ⅲ and Ⅳ[(246. 89 ± 114. 34) pg/ml vs ( 152. 82 ± 54. 55 ) pg/ml, P < 0. 05] .There was positive correlation between the level of GRO-α and r-AFS ( r = 0. 439, P < 0. 05 ), but no cor-relation between the level of VEGF and r-AFS ( r = -0. 210, P >0.05). There was no correlation be-tween peritoneal fluid levels of GRO-α and VEGF in the EMs group ( r =-0. 05, P>0. 05). Conclusion Compared with control group, the levels of GRO-α and VEGF in peritoneal fluid of women with endome-triosis were significantly increased, which indicated that the increased GR0-α and VEGF secretion involved in the pathogenesis of this disease. There was correlation between the peritoneal fluid level of GRO-α and the degree of EMs.
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Objective To investigate the expression of 5-lipoxygenase (5-LOX) in endometriosis and analyze the relationship of 5-LOX and angionesis in endometriosis. Methods Immunochemical method was used to determine the expression of 5-LOX, VEGF, and the value of MVD in ectopic endometrium (ec-topic group) and eutopic endometrium (eutopic group) of 32 patients with EMs and in eutopic endometrium ( control group) of 25 patients without EMs. Result The expressions of 5-LOX and VECF and the value of MVD in ectopic groups were 0. 43 ± 0.09,0. 39 ±0.07 and 0.40 ± 0.09, respectively, which were higher than that of eutopic group and control group. The expressions of 5-LOX and VEGF and the value of MVD in eutopic group were 0. 35 ± 0. 08, 0.30 ± 0. 06 and 0. 33 ± 0. 08, respectively, which were higher than that of control group ( P <0.05). In ectopic group, the expressions of 5-LOX and VEGF, and the value of MVD in stage III/IV, were much higher than that of stage I/II ( P <0. 05); the expressions of 5-LOX and VEGF and the value of MVD were related to the severe of endometriosis through Spearman correlation analysis. The expressions of 5-LOX were shown to be significantly related to the expressions of VEGF and the value of MVD in ectopic and eutopic groups ( P <0. 05). Conclusion There is a high expression of 5-LOX in endometriosis, suggesting that 5-LOX may be participate in the pathogenesis of endometriosis. 5-LOX may promote angiogenesis of EMs through up - regulating the expression of VEGF.
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Objective To explore the changes of frequency, BAFF expression and sensitivity to apoptosis of CD19 + CD5 + B cells in endometriosis patients and investigate the relationship between CD19 +CD5 + B cells and endometriosis. Methods Flow cytometry and quantitative PCR were used to assess the frequency, BAFF expression and sensitivity to apoptosis of CD19+ CD5 + B cells in the peripheral blood,peritoneal fluids and endometrium biopsies of 39 patients with endometriosis and 31 patients with myoma of uterus ( control subjects). Results: All 39 endometriosis patients had higher frequencies ( all P ＜ 0. 01 ) of CD19 + CD5 + B cells in the peripheral blood, peritoneal fluids and endometrium biopsies than that in 31 control subjects. CD19 + CD5 + B cells from endometriosis patients expressed higher BAFF, and were more resistant to CD95L-induced apoptosis than the cells from control subjects ( P ＜ 0. 01 ). Conclusion CD19 + CD5 + B cells might play an important role in the pathogenesis of endometriosis through higher BAFF expression and more resistance to CD95L-induced apoptosis.
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Objective To explore the role of TGF-βand TGIF in the pathogenesis of endometriosis. Methods The expression of TGF-β and TGIF was detected by immunohistochemistry method in the ectopic and eutopic endometrium of 30 cases with endometriosis (ec-topic endometrium group and eutopic endometrium group) and in the normal endometrium of 40 cases without endometriosis (control group). Result The expression of TGF -β in ectopic endometrium group was significantly higher than that in eutopic endometrium group and control group (P < 0.05). There was no significant difference in the expression of TGF- βbetween eutopic endometrium group and control group(P > 0.05). The expression of TGIF in ectopic endometrium group was significantly lower than that in eutopic endometrium group and control group( P <0.05). There was no significant difference in the expression of TGIF between eutopic endometrium group and control group(P > 0.05). There were negative correlation between the expressions of TGF - β and TGIF in ectopic endometrium group and eutopic endome-trium group(rs= - 0.769, - 0.549, P < 0.05). Conclusion The abnormal expression of TGF-β and TGIF in ectopic endometrium of pa-tients with endometriosis may be associated with the genesis and progression of endometriosis.