ABSTRACT
Objective To evaluate the relationship between serum levels of leptin, endothelin and diabetic retinopathy in type 2 diabetes patients. Methods Leptin, endothelia, FBG, PBG, HBA1C, CHOL, TG and other clinical characteristics were tested in 80 type2 diabetes patients and 30 control case. All diabetes patients were divided into three group: non-diabetic retinopathy (NDR), non-proliferative diabetic retinopathy (NPDR) and proliferative diabetic retinopathy(PDR) according to the examination results of fundus. Correlations between levels of serum leptin and other parameters were analyzed. Results Plasma level of leptin in the type 2 diabetes with PDR group[(17.41±5.81)μg/L] were higher than that in control, NPDR and NDR group (P＜0.01). Plasma level of ET in the type 2 diabetes with PDR group[(80.68±13.57)mg/L] were higher than that in control, NPDR and NDR group (P＜0.01). The serum leptin levels were positively correlated with BMI(r＝0.468,P＜0.01). Conclusions Serum leptin and endothelin levels were elevated in patient with diabetic retinopathy as diabetic retinopathy aggravated and serum level of leptin and endothelin raised.
ABSTRACT
Objective To explore influence of hypertriglyceridemia on serum NO ET-1 and the grade of the pathology severity in rats with severe acute pancreatitis. Methods 36 SD rats were randomly divided into 2 groups, group A (HLSAP group) , group B (SAP group). Severe acute pancreatitis was constructed by retrograde injection of 5% na-taurocholate. Blood samples were taken from all subjects to measure triglyceride, ET-1 and NO, pancreatic tissue samples were taken from head of pancreas and stained with H. E. , the degree of pancreatic damage was observed according to the point score of Schmidt and Pozsar's methods. Results In group A, the concentration of ET-1 increased more obviously than that in group B at 4 hour and 8 hour period(P ＜0. 05). The concentration of NO declined both in group A and group B at 12 hours period,but it had a great decline in group A. Animals with hyperlipidemia and severe acute pancreatitis developed significantly higher(P ＜0. 05) ET-1 than the animals of the non-hyperlipamic severe acute pancreatit group in 4 hours and 8 hours period. NO declined in group A and group B at 12 hours period, group A have significantly higher(P ＜0. 05) decline than group B in NO. The histological degree of pancreatic damage were significantly higher in group A than that in the group B at all times. Conclusions Mircrocirculation disorder had existed disorder in the early of SAP. Hypertriglyceridemia could incrase ET-1 and NO earlier and higher in severe acute pancreatitis, and then decline in the late stage. Hypertriglyceridemia intensified the pathologichistological degree of pancreatic damage.
ABSTRACT
Objective To explore the effect of nitroglycerin on ET/NO, TXA2/PGI2 and pancreas pathomorphology changes in severe acute pancreatitis (SAP) rats. Methods Sixty SD rats were random divided into five groups, including control group (A group, n = 12) and experimental group(B,C,D and E group, n = 12). The SAP was induced by injection of 5% sodium taurocholate through retrograde common biliopancreatic ducts via duodenal papilla with epidural catheter. Group C, D and E were intravenously injected with nitroglycerin 0.5μg/kg/min,1μg/kg/min and 2μg/min in 30 min respectively, and group A and B was injected with Sodium Chloride 0.5ml. The indexes of changed pathomorphology and ET/NO, TXB2/6-keto-PGF1a, were determined at the 6th and 12th hour after operations, respectively. Results The specimen data of the 6th and 12th hour displayed that the indexes of changed pathomorphology, ET, ET/NO, TXB2, and TXB2/6-keto-PGF1a of the group C,D and E degraded respectively, compared to group B(P < 0.05). Conclusion The nitroglycerin could degrade ET, ET/NO,TXA2 and TXA2/PGI2, improve the microcirculation of pancreas, and delay the pathological inflammation change in SAP rats.