ABSTRACT
O objetivo deste estudo foi avaliar o efeito da suplementação de óleo de cártamo sobre peso corporal, perfis lipídico e glicídico e capacidade da atividade antioxidante enzimática em ratos Wistar induzidos à obesidade por dieta hiperlipídica. Utilizaram-se 28 ratos adultos, machos, distribuídos em quatro grupos experimentais: Grupo Controle e dieta normolipídica (GCN); Grupo controle e dieta hiperlipídica (GCH); Grupo Óleo de cártamo e dieta normolipídica (GNOC) e Grupo Óleo de cártamo e dieta hiperlipídica (GHOC). Durante 30 dias, observaram-se consumo alimentar e peso corporal e, ao final do experimento, glicemia, perfil lipídico, formação de Substâncias Reativas ao Ácido Tiobarbitúrico (TBARS) e atividade das enzimas antioxidantes: Catalase (CAT), Glutationa Peroxidase (GHS-Px) e Superóxido dismutase (SOD). Os resultados demonstraram que o consumo alimentar foi menor nos grupos tratados com óleo de cártamo e a média do peso corporal foi inferior no GNOC e superior no GCH. Houve aumento das concentrações de Lipoproteína de Alta Densidade (HDL-colesterol) no GNOC. Os grupos GCH e GHOC demonstraram médias de TBARS superiores a dos grupos GCN e GNOC. O GCH apresentou menor GSH-Px e o GHOC, menor atividadede CAT quando comparado aos grupos GCN e GNOC. Concluiu-se que a suplementação com óleo de cártamo reduziu consumo alimentar e o ganho de peso corporal dos animais aumentou o HDL-colesterol no grupo com dieta normolipídica, mas não foi capaz de prevenir o aumento da peroxidação lipídica induzida pela dieta hiperlipídica, embora tenha evitado a redução da atividade antioxidante enzimática representada pela GHS-Px...
The aim of this study was to assess the effects of safflower oil supplementation on body weight, glucose and lipid profiles and the antioxidant enzyme activities in Wistar rats with high-fat diet-induced obesity. The 28 adult male rats were divided into four groups: Control Group fed on Standard (low-fat) Diet (GCN); Control Group fed on High-fat Diet (GCH); Safflower Oil Group with Standard Diet (GNOC) and Safflower Oil Group with High-fat Diet (GHOC). Food intake and body weight were monitored for 30 days. At the end of the experiment, blood was collected for assays of blood glucose, lipid profile, formation of thiobarbituric acid reactive substances (TBARS) and antioxidant enzyme activities: viz. catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD). The results showed that food intake was lower in the groups treated with safflower oil and that the average body weight was lower in GNOC and higher in GCH. There were increased concentrations of high-density lipoprotein (HDL cholesterol) in GNOC. Both high-fat groups (GCH and GHOC) showed higher average TBARS than GCN and GNOC. GCH showed lower GSH-Px activity and GHOC showed lower CAT activity than GCN and GNOC. It was concluded that supplementation with safflower oil reduced the food intake and body weight of the animals, and increased the HDL-cholesterol in the normal diet group, but did not prevent the increase in lipid peroxidation induced by the high-fat diet, despite having prevented the reduction of the antioxidant enzyme activity of GSH-Px...
Subject(s)
Animals , Male , Rats , Carthamus , Dietary Fats , Eating , Obesity , Phytotherapy , Rats, WistarABSTRACT
PURPOSE: To compare the antioxidant enzyme activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the levels of lipid peroxidation product malondialdehyde (MDA) in blood samples of thyroid cancer patients compared to healthy controls. METHODS: 43 control subjects (mean age 44±13 years) and 43 patients (43±13 years) presented with multinodular goiter whose fine needle aspiration revealed malignant cytology were included into this study. The SOD, MDA and GSH-Px activities were measured in control subjects, and before/20 days after thyroidectomy in thyroid cancer patients. RESULTS: SOD activities of pre-thyroidectomy, post-thyroidectomy and control groups were not different (p>0.05). Before thyroidectomy GSH-Px activities were lower (p<0.05) and MDA levels were higher (p<0.05) than the control group. In post- thyroidectomy, GSH-Px activity (p<0.05) increased, and MDA levels (p<0.05) decreased compared to prethyroidectomy levels. After thyroidectomy GSH-Px activity was significantly higher than the control group (p<0.05). Although post-thyroidectomy MDA levels significantly decreased, they were still higher than the control group (p<0.05). CONCLUSION: The superoxide dismutase does not seem to change with thyroid cancer and thyroidectomy but both antioxidant glutathione peroxidase and lipid peroxidation product malondialdehyde do. These preliminary findings may point out oxidant/antioxidant imbalance associated with thyroid cancer.