ABSTRACT
To follow up the change of melanocytes to keratinocytes ratio in stratified epidermis reconstructed by mixed culture of these cells, the author investigated the ratio of melanocyte to keratinocyte in the basal layer of reconstructed epidermis after induction of stratification for 1 week with cocultured melanocyte and keratinocyte in various ratio. Initial mixing ratios of melanocyte to keratinocyte were 1 : 10, 1 : 20, 1 : 40, 1 : 80. Post ratios were changed to 1 : 6.7, 1 : 10.7, 1 : 12.6, 1 : 13.8 respectively after 1 week. Eight time of initial gap reduced to two times and melanocyte to keratinocyte ratio converged to 1 : 10. To know the regulation mechanism, the author investigated the ratio of basal melanocytes to suprabasal melanotytes. The ratios were nearly same by showing 0.142, 0.140, 0.118, 0.104 respectively. These results suggest that the ratio of melanocyte to keratinocyte in reconstructed stratified epidermis in vitro was not related with initial mixing ratio and was regulated by proliferation of melanocytes controled by corresponding keratinocytes.
Subject(s)
Cell- and Tissue-Based Therapy , Coculture Techniques , Epidermis , Follow-Up Studies , Keratinocytes , MART-1 Antigen , MelanocytesABSTRACT
Objective To study effect of diclofenac sodium on content of prostaglandin E 2 KC/MC ratio and hyperpigmentation of the full-thickness skin autografts. Methods Content of PGE 2 was determined by radioimmune analysis method, and kinetic changes of KC/MC ratio were observed with histological and histochemical methods. Results In the operation control group, increase of KC/MC ratios was accompanied by of PGE 2 content at the early stage of the experiment; and the KMR became smaller than that in normal control group at the end of the experiment. While in the diclofenac soduim group, KMR was reduced due to the less damage of MC at the early stage, with decrease of PGE 2. Accordingly, KMR became larger at the late period of the experiment, and color of the autografts became lighter. Conclusion Application of diclofenac sodium on the grafts just after transplantation may reduce the damage of inflammation to the epidermal melanin units, control their proliferation and decrease the value of KMR, which lightens the color of the grafts.