ABSTRACT
Objective To optimize ultrasonic-assisted extraction Epimedin C and Icariin from Epimedii Wushanensis Herba by response surface methodology. Methods On the basis of single factor tests, Box-Behnken experimental design and response surface methodology were adopted to optimize extraction conditions with the concentration of ethanol, ultrasonic time and solid-liquid ratio as factors. HPLC was used to determine the content of Epimedin C and Icariin from Epimedii Wushanensis Herba. Results Optimal ultrasound-assisted extraction technology was as following: the concentration of ethanol was 73%; the ultrasonic time was 22 min; the ratio of liquid to material was 1:32. The contents of Epimedin C and Icariin from Epimedii Wushanensis Herba were 15.90% and 0.75%, respectively. Conclusion This extraction technology can improve the extraction efficiency of Epimedin C and Icariin from Epimedii Wushanensis Herba, which is in accordance with predicted value.
ABSTRACT
Objective: To estabolish te fertilization index of nitrogen (N), phosphorus (P), and potassium (K) and regression model of Epimedii Wushanensis Herba, and to explore the optimization of fertilization. Methods: The design of the fertilizer element 3 and factor 4 was introduced, the effect test of N, P, and K fertilizers was laid out and the test data were collected and analyzed statisticly. Results: An empirical model of the relationship between Epimedii Wushanensis Herba and the contents of Icariin and epimedin C to N, P, and K were established and optimized to analyze the interaction among the tested factors. Conclusion: Yield and quality of Epimedii Wushanensis Herba are lower significantly without one of N, P, and K and the optimization treatment efficiency is N2P2K2. Single-factor analysis shows that the yield and the contents of active ingredients Icariin and epimedin C increase and then decrease steadily with the increase of the N, P, and K application. The first order interaction analysis indicates that there exists an interaction range between N and P, N and K, P and K. The synergistic and antagonistic effects are shown in the lower and higher.