ABSTRACT
Eighteen patients with early cancer and precancerous lesions of esophagogastric junction underwent multiband mucosectomy (MBM) in Yangzhou First People's Hospital from January 2013 to February 2014.The clinicopathological data of patients were analyzed retrospectively and the short-term efficacy and safety of MBM were evaluated.Operations were successful in all 18 cases.The mean operative time was 35.8 min.Intraoperative bleeding occurred in 3 cases and successfully managed by endoscopic hemostasis.Small perforation occurred in 1 patient and was closed by metal clips.Pathological examination showed mucosal cancer in 5 cases,high-grade intraepithelial neoplasia in 8 cases and low-grade intraepithelial neoplasia in 5 cases.No relapse of cancer was found during follow-up.Results indicate that MBM is an effective and safe method in treatment of early cancer and precancerous lesions in the esophagogastric junction.
ABSTRACT
Objective To investigate the mechanisms of Mal gene down regulated in human esophageal cancer. Methods We amplified the 5′regulatory region of Mal gene in normal placenta tissue and human esophageal cancer cell lines EC9706、EC8712、EC109 by optimization the PCR methods, cloned and sequenced the PCR products and compare the sequence. Results Human esophageal cancer cell lines EC9706、EC8712、EC109 all have the 654 th of 5′regulatory region of Mal gene T to C changes, corresponding codon changed from TCC to CCC, and the amino acid which it codes changed from serine to proline; EC9706 also has the 657 th of 5′regulatory region of Mal gene T to C changes, corresponding codon changed from TGC to CGC, the amino acid which it codes changed from cysteine to arginine; EC8712 has the 139 th of 5′regulatory region of Mal gene A to G changed, corresponding codon changed from GGA to GGG, the amino acid which it codes remains glycine. Conclusion Our results suggested there are may point mutation in 5′ region of Mal gene in human esophageal cancer.
ABSTRACT
The Eca-109 cell line (human cancer cell line of the esophagus) and CHO cell line (Chinese hamster ovary cell line) were used in the present experiment. Three groups of the fusion cells induced by PEG were obtained. These groups were: (1) the cells formed by the fusion of Eca-109 and Eca-109. (2) the fusion of CHO and CHO. (3) the hybrids of Eca-109 and CHO. The third group was demonstrated by autoradiography after incorporated H~3-thymine. The results indicate that the number of megalonucleated cells increases more and more, while the number of multinucleated cells gradually decreases in 10 days after the treatment by PEG in all groups.The forms of fusion cells of Eca-109 and Eca-109 or CHO and CHO similar to those of parent cells. Heterogeneous binucleated cells have the same form as one of parent ceils or as transitional form between two parent cells. The multinucleated cells have various shapes.Histochemical observations have shown that reactions of lactate dehydrogenase (LDH), glutamate dehydrogenase (GDH), malate dehydrogenase (MDH) in the fused cells were stronger than mononucleated cells. The level of activity of nonspecific esterase (NSE) in heterokaryon was intermediate between two parent cells i.e. higher than CHO and lower than Eca-109.