Molecular identification and efficacy analysis of herbs at Orussey Herbal Market, Phnom Penh, Cambodia / 中国中药杂志

Cambodia is rich in medicinal plant resources. One hundred and thirty-three medicinal material samples, including the hole herb, root, stem/branch, leaf, flower, fruit, seed, and resin, were collected from the Orussey Herbal Market in Phnom Penh, Cambodia, and then authenticated by ITS and psbA-trnH. A total of 46 samples were identified based on ITS sequences, belonging to 24 families, 40 genera, and 42 species. A total of 100 samples were identified by psbA-trnH sequences to belong to 42 families, 77 genera, and 84 species. A total of 103 samples were identified by two DNA barcodes. According to the morphological characteristics of the medicinal materials, 120 samples classified into 50 species, 86 genera, and 86 families were identified, and the majority of them were from Zingiberaceae, Fabaceae, and Acanthaceae. Such samples have been commonly used in traditional Cambodian medicine, Ayurvedic medicine, Unani medicine, traditional Chinese medicine, and ethnomedicine, but different medical systems focus on different functional aspects of the same medicinal material. The results of this study have demonstrated that DNA barcoding has a significant advantage in identifying herbal products, and this study has provided basic data for understanding the traditional medicinal materials used in Cambodia.

Ethnomedicinal Uses Of Plants: A Study Among The Juang Tribe Of Keonjhar District Of Odisha

An Ethno-Medicinal survey was carried out in some villages of Juang community in Keonjhar district of Orissa to evaluate the plants used for medicine by them. The investigation was carried out in Juang villages with participant observation, Interview method, case study, snowball sampling and administration of Survey schedules. The study enumerated 32 species belonging to 21 families that are used by them for treatment of common ailments and Healthcare. The plants used for traditional medicines are mostly collected from the forest. It has been revealed that although the Juang concept of disease and treatment revolves around a religious beliefs and practices, they depend on plant medicine to a larger extent, although they are using modern medicine in the current scenario. It has been understood that some of the medicinal plants have become scares in the area due to industrialisation and other activity. Documentation of traditional knowledge on the economy and the uses of these plants may contribute to the germplasm conservation efforts as well as for the new drug development.

Pharmacological importance of Manilkara zapota and its bioactive constituents / Importancia farmacologica de Manilkara zapota y sus constituyentes bioactivos

Manilkara zapota (Sapotaceae), commonly known as Sapodilla, is widely known for its delicious fruit. Various parts of this plant are also used in folk medicine to treat a number of conditions including fever, pain, diarrhoea, dysentery, haemorrhage and ulcers. Scientific studies have demonstrated analgesic, anti-inflammatory, antioxidant, cytotoxic, antimicrobial, antidiarrheal, anti-hypercholesteremic, antihyperglycemic and hepatoprotective activities in several parts of the plant. Phytochemical studies have revealed the presence of phenolic compounds including protocatechuic acid quercitrin, myricitrin, catechin, gallic acid, vanillic acid, caffeic acid, syringic acid, coumaric acid, ferulic acid, etc. as main constituents of the plant. Several fatty acids, carotenoids, triterpenes, sterols, hydrocarbons and phenylethanoid compounds have also been isolated from M. zapota. The present review is a comprehensive description focused on pharmacological activities and phytochemical constituents of M. zapota.

Manilkara zapota (Sapotaceae), comúnmente conocida como Sapodilla, es ampliamente conocida por su delicioso fruto. Variadas partes de esta planta se usan en medicina popular para tratar una serie de afecciones, como fiebre, dolor, diarrea, disentería, hemorragia y úlceras. Estudios científicos han demostrado actividad analgésica, antiinflamatoria, antioxidante, citotóxica, antimicrobiana, antidiarreica, antihipercolesterolémica, antihiperglucémica y hepatoprotectora en diferentes partes de la planta. Los estudios fitoquímicos han revelado la presencia de compuestos fenólicos que incluyen ácido protocatechúico, quercitrina, miricitrina, catequina, ácido galico, ácido vanílico, ácido cafeico, ácido sirínico, ácido cumárico, ácido fúnico y ácido ferúlico como componentes principales de la planta. Varios ácidos grasos, carotenoides, triterpenos, esteroles, hidrocarburos y compuestos feniletanoides también han sido aislados de M. zapota. La presente revisión es una descripción exhaustiva centrada en las actividades farmacológicas y los constituyentes fitoquímicos de M. zapota.

A review of five traditionally used anti-diabetic plants of Bangladesh and their pharmacological activities

Plants are used traditionally throughout the globe to treat various diseases. Traditionally used medicinal plants are an essential part of the health sector in Bangladesh due to its abundance of a vast source of ethno-medicine. Rural people from developing country like Bangladesh are greatly dependent on traditional source of medicine. The prevalence of diabetes mellitus is increasing from recent years; therefore various researches are going on to discover better medicine to treat this disease. This study has focused on five plants which are Andrographis paniculata, Ageratum conyzoides, Swertia chirata, Terminalia arjuna, and Azadirachta indica to find out their traditional formulation as anti-diabetic medicine and their pharmacological activity has also been explored through literature search. The available information about traditional anti-diabetic uses of these plants and their pharmacological activities were collected from various electronic sources like Pubmed, SciFinder, Elsevier, Springer, Scopus, Scirus, Science Direct, Google Scholar and Web of Science apart from these locally available books and peer reviewed journal were also used to collect information. This study will help to strengthen the relation between traditional medicine, pharmacology and drug development. A clue may be found from the information provided this review to discover new and better anti-diabetic drugs.

A review of five traditionally used anti-diabetic plants of Bangladesh and their pharmacological activities

Plants are used traditionally throughout the globe to treat various diseases. Traditionally used medicinal plants are an essential part of the health sector in Bangladesh due to its abundance of a vast source of ethno-medicine. Rural people from developing country like Bangladesh are greatly dependent on traditional source of medicine. The prevalence of diabetes mellitus is increasing from recent years; therefore various researches are going on to discover better medicine to treat this disease. This study has focused on five plants which are Andrographis paniculata, Ageratum conyzoides, Swertia chirata, Terminalia arjuna, and Azadirachta indica to find out their traditional formulation as anti-diabetic medicine and their pharmacological activity has also been explored through literature search. The available information about traditional anti-diabetic uses of these plants and their pharmacological activities were collected from various electronic sources like Pubmed, SciFinder, Elsevier, Springer, Scopus, Scirus, Science Direct, Google Scholar and Web of Science apart from these locally available books and peer reviewed journal were also used to collect information. This study will help to strengthen the relation between traditional medicine, pharmacology and drug development. A clue may be found from the information provided this review to discover new and better anti-diabetic drugs.

Anti-mycobacterial assessment and characterization of 5-O-caffeoylquinic acid methyl ester and rutin from Pavetta crassipes.

Pavetta crassipes leaf (Fam. Rubiaceae) is used as part of a combination herbal remedy for the treatment of tuberculosis (TB) and other respiratory infections in Nigerian ethno medicine. However, little scientific data is available to support the use in ethnomedical therapy so the objective of the study was to assess the anti-tubercular property and to identify the bioactive components. The dried powdered leaf was sequentially extracted with solvents to obtain hexane, dichloromethane, methanol and water extracts. Following which, the extracts were then screened against Mycobacterium aurum, a rapidly growing saprophytic mycobacterium species for activity. The methanol extract exhibited inhibitory activity at an MIC value of 250 µg/mL against M. aurum and two known polyphenolic compounds were isolated as 5-O-caffeoylquinic acid methyl ester and quercetin-3-rutinoside (rutin). Reversed phase semi-preparative HPLC, mass spectrometry and 1H and 13C NMR techniques were utilized in isolating and characterizing the two components. The assignments of the structures were consistent with data from the literature. The study has shown that the methanol extract has some activity and hyphenation of LC-MS can be used for the isolation of polyphenols from the methanol fraction without a rigorous purification process.

Identification of medicinal plants of Urmia for treatment of gastrointestinal disorders

The gastrointestinal tract is one of the most important organs of the human body and is vulnerable to different diseases. Available drugs often have low efficacy or are associated with many adverse effects. Therefore, alternative drugs are necessary to treat gastrointestinal complications. This study intended to identify medicinal plants in Urmia, Iran, that can affect common gastrointestinal disorders and diseases. Data was collected from public resources via interviews and questionnaires applied from April to June 2013. Herbarium specimens were collected from the region and authenticated by a botanist. A total of 41 indigenous medicinal plants from the Urmia region, belonging to twenty families, have a traditional medicinal role in the treatment of parasitic and infectious diseases, diarrhea, reflux, gastroenteritis, peptic ulcer, constipation, bloating, among other gastrointestinal tract disorders. Analysis showed that most plants affecting the gastrointestinal tract belonged in the Asteraceae family (24%). The most used part of the plants was the seed at 17%. Decoction at 65% was the most popular form of treatment used. Some of the medicinal plants discussed in this article have new implications presented for the first time. Pharmacological studies on the therapeutic effects of the indigenous plants mentioned in this study are necessary in order to investigate their claimed clinical effects and the use of their effective compounds to produce natural and useful drugs. Currently, there is no data on the herbal plants used to treat gastrointestinal disorders in northwestern Iran. Therefore, these findings are important for the management of gastrointestinal disorders and to conduct future studies on traditional medicine for drug development.

Diversity of Potent Ethno-Medicinal Flora for Headache from North-Tarai Forests of (U. P.) India.

An ethno-medicinal survey was undertaken in the North Tarai Forests of Uttar Pradesh for the documentation of ethno-medicinal information of plants and herbs used for the treatment of various ailments in the area by traditional healers as well as rural inhabitants. The North Tarai region of U. P. has well blessed phytodiversity in its green natural forests as well as rich ethnic and rural communities. The rural inhabitants of the area are still far from modern civilization so they are totally dependent on forests for their various needs specially for healthcare. Due to the presence of rich traditional knowledge of medicine and great faith and belief on Ayurvedic and Siddha system of medicine the rural inhabitants use herbal medication. During the ethno medicinal survey we found one hundred forty plant species in which one hundred ten plant species belonging to ninety five genera representing fifty one families were reported which were being used for the treatment of headache. The present work was undertaken for the treatment of headache. The investigation was performed by collection of medicinal plants in consultation with local healers, rural dwellers, as well as medicine venders. Questionnaire method was adopted for the documentation of ethno medicinal information. Various medicinal plant parts viz. leaves, flowers, bark, latex, seeds, rhizome, root, as well as whole plant part were being used as ethnomedicine. Mostly medicines were used externally while somewhere also being used internally. For each plant species details of scientific name, author name, family name and vernacular name as well as method and mode of medicine administration are described in the text. In conclusion the North-Tarai region of U. P. disposes great diversity of medicinal plants as well as large phytotherapeutic knowledge which are to be scientifically investigated and needed to be conserved.

Chemical constituents of Dai ethno-medicine Rhinacanthus nasutus / 中草药

Objective To study the chemical constituents of the Dai ethno-medicine Rhinacanthus nasutus.Methods The chemical constituents had been spearated with chromatography and their structures were determined by spectral analyses.Results Nine compounds and two mixture were isolated and identified as C26—C32 alkyl mixture(Ⅰ),?-monopalmitin(Ⅱ),palmitic acid(Ⅲ),stearic acid(Ⅳ),lignoceric acid(Ⅴ),?-sitosterol(Ⅵ),lupeol(Ⅶ),p-hydroxy benzoic acid(Ⅷ),daucosterol(Ⅸ),?-ethyl-D-idosopyranoside(Ⅹ),and mixture of KNO3 and NaNO3(Ⅺ).Conclusion Compound Ⅹ is a new artificial compound during isolation and compounds Ⅰ—Ⅴ,Ⅷ—Ⅺ are isolated from R.nasutus for the first time.