ABSTRACT
Objective:To develop an HPLC-DAD-ELSD method for the determination of nitidine chloride, 5-ethoxychelerythrine, bergeninum and ardisiacrispin A in Shangtong tinctures ( STD) . Methods: A Hypersil C18 column was used as the chromatographic column, the flow rate was 0. 8 ml·min-1 . For nitidine chloride and 5-ethoxychelerythrine, the mobile phase A consisted of acetoni-trile,the mobile phase B consisted of 0. 1% formic acid-triethylamine (pH 4. 5),and the DAD detection wavelength was at 273 nm. For bergeninum and ardisiacrispin A, the mobile phase consisted of methanol-water(25∶75), the temperature of drift tube was set at 95℃, and the gas flow (N2) was set at 2. 5 SLPM·min-1. Results:There was a good linear relationship between the concentration and peak area for nitidine chloride and 5-ethoxychelerythrine within the range of 0. 021-0. 426 μg (r=0. 999 5) and 0. 075-1. 494 μg (r=0. 999 8), respectively. The average recovery was 99. 22%(RSD=0. 64%) and 98. 61%(RSD=0. 46%), respectively. There was a good linear relationship between the concentration and peak area for bergeninum and ardisiacrispin A within the range of 0. 215-4. 304 μg(r=0. 999 3) and 0. 286-5. 728 μg(r=0. 999 7), respectively. The average recovery was 99. 15%(RSD=0. 77%) and 99. 25%(RSD=0. 56%) accordingly. Conclusion:The method is accurate, sensitive and reproducible, and can be used in the de-termination of nitidine chloride, 5-ethoxychelerythrine, bergeninum and ardisiacrispin A in STD.
ABSTRACT
OBJECTIVE: To develop a RP-HPLC method for simultaneous determination of magnoflorine, hesperidin, nitidine chloride, ethoxychelerythrine and toddaloactone in Zanthozylum nitidum (Roxb) DC.f. fastuosum How ex Huang. METHODS: The RP-HPLC system consisted of a Diamonsil C18 column (4.6 mm×250 mm, 5μm) with the mobile phase of acetonitrile solution-water solution (containing 0.2% phosphoric acid and 0.2% triethylamine) for gradient elution. DAD detector was used and the detection wave lengths were 273, 283 and 328 nm. The flow rate was 1.0 mL·min-1 and the column temperature was 30°C. For different constituents, external standard method was used with the peak area at the maximum absorption wavelength as the quantitative index. RESULTS: The liner ranges of magnoflorine, hesperidin, nitidine chloride, ethoxychelerythrine and toddaloactone were 0.0957-1.3391 μg (r=0.9995), 0.3189-2.1260 μg(r=0.9998),0.0397-0.2648 μg (r=0.9995), 0.1004-1.0040 μg(r=0.9999), and 0.1080-2.1600 μg (r=0.9999), respectively. The average recoveries (n=6) were 100.2%, 99.8%, 97.1%, 98.8% and 101.6% (n=6) Respectively. CONCLUSION: The method is accurate, simple, rapid, and reproducible for the determination of magnoflorine, hesperidin, nitidine chloride, ethoxychelerythrine and toddaloactone in Zanthoxylum nitidum (Roxb) DC.f. fastuosum How ex Huang. The determination result can be used as a reference for the reasonable medication, quality control and further study of Zanthoxylum nitidum (Roxb) DC.f. fastuosum How ex Huang.