ABSTRACT
Background: Ethanolic, aqueous, petroleum ether and ethyl acetate extracts of leaves of Chromolaena odorata were studied for their antimicrobial activity. Twelve bacterial species, including six Gram-positive and six Gram-negative bacteria viz. Vibrio cholerae, Shigella sonnei, Klebsiella pneumoniae, Salmonella paratyphi A, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus citreus, methicillin-resistant S. aureus, nonpathogenic Mycobacterium gordonae and Mycobacterium fortuitum along with fungi Candida albicans and Aspergillus niger were studied. Methods: This study involved antibiotic sensitivity testing of extracts against a wide spectrum of micro-organisms, to determine the antimicrobial activity of the plant. Results: Initial phytochemical testing unveiled the chemical constituents of C. odorata as saponins, flavonoids, tannins, steroids and proteins. The zone of inhibition for the respective extracts was compared with the zone of the standard antibiotic and was found to be quite effective. Conclusions: The ethanolic extract of C. odorata exhibited significant antimicrobial activity. The plant extracts could be used to treat resistant form of prevailing infections. Successful antimicrobial drugs can be developed out of these extracts if specific compounds are isolated and purified.
ABSTRACT
Objective To study the analgesic effect of Eupatorium odoratum L and its mechanism .Methods Mice were random‐ly divided into 6 groups .Swinging tail method in spinal cord destroyed mouse ,hot‐plate and twisted body method in naloxone blocked mouse ,and formalin‐induce inflammatory pain method were done to investigate the analgesic effect of Eupatorium odoratum L .Each pain index under each experiment condition was determined after intra gastric administration of each group of mice .The concentration of NO and PGE2 in serum and brain were measured to study its mechanisms .Results High‐dose of Eupatorium odor‐atum L significantly enhanced pain threshold in either spinal cord destroyed or naloxone blocked mouse with pain induced by hot and acetic acid(P<0 .05) .High and middle dosage groups of Eupatorium odoratum L markedly reduced the first‐and second‐phase re‐sponses of formalin‐induced inflammatory pain mouse(P< 0 .05) ,and decreased the writhing times and licking rear feet times caused by twisted body method(P<0 .05) .The level of NO and PGE2 in serum and brain of mouse induced by formalin were both decreased(P<0 .05) .Conclusion Eupatorium odoratum L has notable analgesic effect ,which may be related to reduce the level of NO and PGE2 in central and peripheral site .
ABSTRACT
By a combination of gas-chromatography and gas-chromatography/mass spectrum, the chemical compositions of volatile oil of Co Lao (Eupatorium odoratum –L.) collected from Nghe An (NA) and Ha Tinh (HT) provinces were identified. Volatile oil contents from NA sample was 0,28%, HT 0,25%. The samples have got yellow color, lighter than water, characterized odor. Volatile oil is a mixture of 60 compounds,-39 in NA samples and 37 in HT. The main components of volatile oil are geijerene, germacrene-D and alpha pinene