ABSTRACT
Objective: To establish the chromatographic fingerprint analysis for the quality control of Eupolyphaga steleophaga with OPA-FMOC by on-line derivatization. Methods: Analysis was completed with a gradient elution of 0.01 mol/L sodium dihydrogen phosphate and 0.01 mol/L sodium tetraborate, pH = 8.2 (A) and methanol-acetonitrile-water was 45:45:10 (B). The wavelength was set at 338 nm from 0-24 min, and at 228 nm from 24-27 min. Efficient and symmetrical peaks were obtained with data flowrate of 1.6 mL∙min. Results: A total of 28 peaks were detected, while 15 ingredients were identified. The 18 batches of samples were classified into two groups based on the results of cluster analysis. The first category was E. sinensis and Steleophaga plancyi; The second category was Opisthoplata orientalis and it was identified as the adulterants. Conclusion: The HPLC fingerprint method is repeatable, feasible in analysis of E. steleophaga and could be used scientifically to evaluate the quality of E. steleophaga with quality control.