Association of agression and angry expression category perception under social exclusion among male students in reform school / 中国学校卫生

Objective@#To explore the relationship between aggression and category perception of angry expression in reform school students under social exclusion, so as to provide reference for the reform school students mental health promotion.@*Methods@#In May 2023, 144 students were randomly selected from a reform school in Guizhou Province, and were divided into high and low aggression groups(77 and 67 students) by Aggression Questionnaire. Cyberball game was used to induce social exclusion and acceptance, subjects were divided into high aggressive exclusion group ( n =42), high aggressive acceptance group ( n =35), low aggressive exclusion group ( n =37) and low aggressive acceptance group ( n =30). All the participants completed the discrimination and identification tasks of category perception paradigm, and the relationship between aggression and category perception of angry expression of reform school students under social exclusion was analyzed by using category turning point, identification curve and analysis of variance.@*Results@#The total score of aggression(97.34±8.00) and four dimensions (physical aggression: 29.75± 4.61, verbal aggression:17.19±2.58, anger:22.29±3.66, hostility:28.10±3.54) in the high aggression group were higher than those in the low aggression group(74.10±9.02，21.09±4.98，14.30±2.66，17.16±3.83，21.55±3.88), and the differences were statistically significant ( t =16.38, 10.85, 6.62, 8.20, 10.59, P <0.01). For identifying the turning point of the fear anger continuum, the social exclusion group（2.58±0.07）was significantly smaller than the social acceptance group（2.79±0.07）( F =4.85, η 2=0.07, P < 0.05 ）, and the social exclusion group had a tendency to shift the category boundary to the fear side. For identifying the slope at the angry happiness continuum category boundary curve, the high aggression group (0.63±0.03) was significantly higher than the low aggression group (0.53±0.03)( F =5.38, η 2=0.08, P <0.05). In the fear anger continuum,the high aggression group[(694.86± 78.29 )ms] reacted more quickly than the low aggression group[(660.70±79.86)ms]( F =5.08, η 2=0.05， P <0.05) In the angry happiness continuum, there was no statistical significance of social exclusion and aggression( P >0.05).@*Conclusions@#The suggests that social exclusion can lead to hostility attribution bias in individuals, while aggression can make individuals more sensitive to angry expression. The mechanisms by which social exclusion and aggression affect expression category perception are independent rather than interactive. The society should give tolerance and acceptance to reform school students, reduce exclusion and discrimination, and the reform education department should correct the aggressive behavior of reform school students and promote their mental health.

A patologia da arte e da exclusão / The pathology of art and exclusion / La patología del arte y de la exclusión

Este trabalho propõe uma reflexão sobre os tênues limites que separam a patologia da criatividade, utilizando-se, para isso, da genialidade da escritora Clarice Lispector. Coloca a ideia da patologia como fenômeno associado à história da misoginia, buscando suas raízes arquetípicas no mito da criação e na figura feminina de Eva. Desenvolve também a relação entre essa misoginia no plano arquetípico e o sentimento de exclusão que vivenciamos nos mais diferentes níveis de relacionamentos.

This study aims to reflect upon the intricate boundaries that separate pathology from creativity, drawing upon the brilliance of writer Clarice Lispector. It explores the concept of pathology as a phenomenon intertwined with the history of misogyny, seeking its archetypal roots in the myth of creation and the feminine figure of Eve. Additionally, it delves into the connection between this archetypal misogyny and the experience of exclusion that we encounter in various levels of relationships.

Este trabajo propone una reflexión sobre los tenues límites que separan la patología de la creatividad, utilizándose, para ello, del genio de la escritora Clarice Lispector. Coloca la idea de la patología como fenómeno asociado a la historia de la misoginia, buscando sus raíces arquetípicas en el mito de la creación y en la figura femenina de Eva. Desarrolla también la relación entre esa misoginia en el plano arquetípico y el sentimiento de exclusión que experimentamos en los más diferentes niveles de relaciones.

Optimization and verification of size exclusion chromatography-high performance liquid chromatography for determination of recombinant human growth hormone-Fc immunofusion protein polymer / 中国生物制品学杂志

@#Objective To optimize and verify the size exclusion chromatography-high performance liquid chromatography(SEC-HPLC) method for the determination of recombinant human growth hormone(rhGH)-Fc immunofusion protein polymer.Methods The multimer content of rhGH-Fc immunofusion protein was detected by SEC-HPLC.The detection conditions(salt concentration,mobile phase pH,flow rate,column temperature and column model) were optimized to observe the separation effect of the target proteins and polymers.The system suitability,specificity,linearity and range,precision,accuracy and limit of quantification of the method were verified.Results The optimized method was to use TSK-gel G2000SW_(x1)column(5 μm,7.8 mm × 300 mm),mobile phase of 50 mmol/L phosphate buffer(pH 6.80),detection wavelength of280 nm,injection volume of 100 μL,flow rate of 0.6 mL/min and column temperature of 45 ℃.The resolution of rhGHFc immunofusion protein and polymer,the theoretical plate number and the tailing factor all met the requirements;the peak time of rhGH-Fc immunofusion protein was the same as that of the control,while the peak time of GH national standard was different from that of the control,and the protein buffer showed no peak;the concentration of rhGH-Fc immunofusion protein was in the range of 0.307～1.842 mg/mL with good linear correlation between the peak area integral value and the injection volume(R~2=0.999 4);the RSD of peak area and purity in repeatability verification were 0.7% and 0.1%,respectively;the RSD of intermediate precision verification was 0.8%;the average recovery rate of accuracy verification was 99.1% with the RSD of 1.9%;the limit of quantification was 6 μg/mL.Conclusion The optimized SEC-HPLC method was used to detect the content of polymer in rhGH-Fc immunofusion protein with improved accuracy,and the column efficiency and separation were in accordance with the relevant requirements of Chinese Pharmacopoeia(Volume Ⅳ,2020edition),which could be used for the detection of polymer content in samples.

Application progress of high performance liquid chromatography in detection of hemagglutinin content in influenza vaccine / 中国生物制品学杂志

@#At present,the most commonly used method for detecting hemagglutinin(HA)content in influenza vaccines is still single-radial immunodiffusion(SRID). However,the preparation of standards required by this method takes a long time,usually 2 ～ 3 months. Therefore,how to quantitatively analyze HA accurately has always been a difficult problem in the detection of HA content in the situation that reference products can not be obtained at the early stage of the pandemic influenza. High performance liquid chromatography(HPLC)has its own characteristics of rapidity,high sensitivity,good repeatability and high accuracy,which can rapidly determine HA content by using different separation principles and has been widely used in the detection of HA content in influenza vaccine. This paper reviewed the research progress of the application of HPLC in the determination of HA content in influenza vaccine.

Quantification of complete viral particles in inactivated avian influenza virus antigen by high performance size exclusion chromatography coupled with multi-angle laser light scattering / 生物工程学报

We developed a method for accurate quantification of the intact virus particles in inactivated avian influenza virus feedstocks. To address the problem of impurities interference in the detection of inactivated avian influenza virus feedstocks by direct high performance size exclusion chromatography (HPSEC), we firstly investigated polyethylene glycol (PEG) precipitation and ion exchange chromatography (IEC) for H5N8 antigen purification. Under the optimized conditions, the removal rate of impurity was 86.87% in IEC using DEAE FF, and the viral hemagglutination recovery was 100%. HPSEC was used to analyze the pretreated samples. The peak of 8.5-10.0 min, which was the characteristic adsorption of intact virus, was analyzed by SDS-PAGE and dynamic light scattering. It was almost free of impurities and the particle size was uniform with an average particle size of 127.7 nm. After adding antibody to the IEC pretreated samples for HPSEC detection, the characteristic peak disappeared, indicating that IEC pretreatment effectively removed the impurities. By coupling HPSEC with multi-angle laser scattering technique (MALLS), the amount of intact virus particles in the sample could be accurately quantified with a good linear relationship between the number of virus particles and the chromatographic peak area (R2=0.997). The established IEC pretreatment-HPSEC-MALLS assay was applied to accurate detection of the number of intact virus particles in viral feedstocks of different subtypes (H7N9), different batches and different concentrations, all with good applicability and reproducibility, Relative standard deviation < 5%, n=3.

Analysis of confidential unit exclusion status of voluntary blood donors in Hefei / 中国输血杂志

【Objective】 To explore the status of confidential unit exclusion(CUE) status among voluntary blood donors in Hefei, so as to provide scientific basis for blood safety. 【Methods】 From 2012 to 2021, 57 out of the 1 158 272 voluntary blood donors in Anhui Blood Center, who requested CUE, were analyzed for population characteristics and their motives in asking for CUE. 【Results】 There were 57(0.004 9%) voluntary blood donors asking for CUE after donation, including 42 males (73.69%) and 15 females (26.32%). All the blood samples were negative in transfusion-transmitted viral marker testing except one positive in syphilis (TP) antibody. The reasons for CUE were as follows: high-risk behaviors (including multiple sexual partners, male-to-male homosexual behaviors, intravenous drug use, etc.) in 23 cases (40.35%), diseases unsuitable for blood donation in 7 cases (12.28%), and other reasons in 14 cases (24.56%). 【Conclusion】 Effective consultation before blood donation is particularly important to ensure blood safety and avoid the waste of blood.

Development and validation of size exclusion column-high performance liquid chromatography for purity determination of bulk material of freeze-dried rabies vaccine for human use / 中国生物制品学杂志

@#Objective To develop and validate size exclusion column-high performance liquid chromatography(SEC-HPLC)for determination of the purity of bulk material of freeze-dried rabies vaccine for human use.Methods Chromatography column TSK-gel G6000PW_(XL)(7.8 mm × 30 cm,13 μm)was used for the determination(column temperature 30 ℃)with mobile phase of 0.1 mol/L PB buffer(pH 7.8)at a flow rate of 0.5 mL/min.The detection wavelength was 280 nm and injection volume was 20 μL.The method was validated for system suitability,specificity,precision and durability and determined for detection limit and quantitation limit,which was applied to analyze bulk material purity of freeze-dried rabies vaccine for human use of 3 batches of Vero cells and 1 batch of human diploid cells.Results The resolution of target protein spectrum peak of bulk material of reference sample and freeze-dried rabies vaccine for human use prepared with two substrates was more than 1.5 with a tailing factor less than 1.5;The blank solvent showed no absorption peak at the position of target protein peak with no interference in the determination;The RSDs of retention time and peak area in precision verification were both less than 2.0%;The quantitative limit was 10 μg/mL,and the detection limit was 4 μg/mL;The reference sample was injected three times continuously at three different detection wavelengths of 278,280 and 282 nm,and the RSDs of retention time and peak area were also less than 2.0%.The purity of 4 batches of freeze-dried rabies vaccine bulk material for human use was all more than 97%.Conclusion The developed SEC-HPLC for determination of the purity of freeze-dried rabies vaccine bulk material for human use showed good specificity,precision and durability,which provided a reliable method for the quality control of human rabies vaccine.

Reversing the PAI-1-induced fibrotic immune exclusion of solid tumor by multivalent CXCR4 antagonistic nano-permeator

Fibrosis is one of the key factors that lead to the immune exclusion of solid tumors. Although degradation of fiber is a promising strategy, its application was still bottlenecked by the side effects of causing metastasis, resulting in the failure of immunotherapy. Here, we developed an antimetastatic polymer (HPA) for the delivery of chemo-drug and antifibrotic siPAI-1 to form the nano-permeator. Nano-permeator shrank after protonation and deeply penetrated into the tumor core to down-regulate the expression of PAI-1 for antifibrosis, and further promoted the sustained infiltration and activation of T cells for killing tumor cells. Moreover, metastasis after fiber elimination was prevented by multivalent CXCR4 antagonistic HPA to reduce the attraction of CXCL12 secreted by distant organs. The administration of stroma-alleviated immunotherapy increased the infiltration of CD8+ T cells to 52.5% in tumor tissues, inhibiting nearly 90% metastasis by HPA in distant organs. The nano-permeator reveals the mechanism and correlation between antifibrosis and antimetastasis and was believed to be the optimizing immunotherapy for solid fibrotic tumors.

¿A quiénes se cuenta? Narrativas sobre las métricas en salud de la población migrante venezolana en una zona de frontera en Colombia / Quem é contado? Narrativas sobre a métrica da saúde da população migrante venezuelana numa zona fronteiriça na Colômbia / Who counts? The health metric narratives of venezuelan migrants on the Colombian border

Resumen Colombia ha sido un país receptor de la migración venezolana originada por la situación sociopolítica del país vecino. Una de las necesidades para dar respuesta a esta crisis es la recolección de datos que permitan visibilizar y monitorear las condiciones de salud de esta población. A partir de un estudio cualitativo centrado en entrevistas y observación etnográfica, el artículo se orienta a caracterizar las variadas dinámicas de inclusión y de exclusión que forman parte de la producción de datos en salud de la población migrante en Cúcuta, un territorio de la frontera colombo-venezolana. A partir de las narrativas de diversos actores que, en el territorio, trabajan directamente en la recolección, el análisis y el uso de datos de salud de la población migrante, el articulo problematiza la construcción de la categoría migrante dentro de las métricas, develando aquello que se visibiliza y aquello que se ignora en el proceso sociotécnico que está detrás de la construcción de estos datos.

Resumo A Colômbia tem sido um país receptor da migração venezuelana devido à situação sociopolítica do país vizinho. Uma das necessidades para responder a esta crise é a coleta de dados que permitam visualizar e monitorizar as condições de saúde desta população. Com base num estudo qualitativo centrado em entrevistas e observação etnográfica, o artigo centra-se na caracterização das diferentes dinâmicas de inclusão e exclusão que fazem parte da produção de dados de saúde sobre a população migrante em Cúcuta, um território fronteiriço colombiano-venezuelano. Com base nas narrativas de vários atores que trabalham diretamente a nível territorial na coleta, análise e utilização de dados de saúde sobre a população migrante, o artigo problematiza a construção da categoria de "migrante" dentro da métrica, revelando o que é visível e o que é ignorado no processo sociotécnico por trás da construção destes dados.

Abstract Colombia has been a destination country of Venezuelan migration originated by the socio-political situation of the neighboring country. One of the needs to respond to this crisis is the collection of data to make visible and monitor the health conditions of this population. Based on a qualitative study centered on interviews and ethnographic observation, the article focuses on characterizing the different inclusion and exclusion dynamics in the production of health data of the migrant population in Cúcuta, a Colombian-Venezuelan border territory. Based on the narratives of various actors who work directly at the territorial level in the collection, analysis and use of health data of the migrant population, the article problematizes the construction of the category of "migrant" within the metrics, revealing what is visible and what is ignored in the socio-technical process behind the construction of these data.

Da exclusão social ao desalento: um olhar "decolonial" / From social exclusion to discouragement: a decolonial look

RESUMO: O objeto central deste artigo, fundamentado na psicanálise, é a problemática da exclusão social. Trata-se de mostrar a importância desse saber em uma reflexão dedicada a situações de precariedade social. Um de seus eixos principais concerne à dimensão de pertencimento, própria ao laço social, tendo em vista, em particular, vividos subjetivos marcados por significativas falhas quanto a essa dimensão. A questão da colonialidade é um operador de relevo neste trabalho, visando ao aprofundamento da relação exclusão/pertencimento. A noção de desamparo e sua modalidade extrema, o desalento, têm grande relevância neste estudo, o qual se ancora na articulação entre subjetividade e universo sócio-cultural.

ABSTRACT: From social exclusion to discouragement: a "decolonial" look. The central object of this article, based on psychoanalysis, is the issue of social exclusion. It is about showing the importance of this knowledge in a reflection dedicated to situations of social precariousness. One of its main axes concerns the dimension of belonging, specific to the social bond, taking into account, in particular, subjective experiences marked by significant flaws in this dimension. The issue of coloniality is a relevant operator in this work, aiming to deepen the relationship of exclusion/belonging. The notion of helplessness and its extreme modality, discouragement, has great relevance in this study, which is anchored in the articulation between subjectivity and the socio-cultural universe.

Quantitation of certolizumab pegol by validated liquid chromatography methods

Abstract Certolizumab pegol (CZP) is a Fab' fragment of the humanized antibody with anti-TNF-α activity that is indicated as therapy for Crohn's disease and rheumatoid arthritis. Using a BioSep-SEC-S3000 column (300 x 4.6 mm i.d., 5 µm particle size), a size exclusion liquid chromatography (SEC) method was developed. Mobile phase A consisted of 100 mM monobasic sodium phosphate and 200 mM sodium chloride (pH 7.0), while mobile phase B was ethanol (95:5, v/v), and the analysis was performed using a diode array detector (DAD) set to 214 nm and a flow rate of 0.5 ml min-1. In addition, a reversed-phase liquid chromatography (RP-LC) method based on gradient elution was developed on a Zorbax 300 SB C18 column (150 mm x 4.6 mm i.d., 3.5 µm particle size) kept at 80 °C. Mobile phase A was 0.1% (v/v) TFA in ultrapure water, and mobile phase B was a mixture of propanol, acetonitrile, ultrapure water and TFA (70 + 20 + 9.9 + 0.1, v/v) operated at a flow rate of 1.0 ml min-1, and DAD was applied at 214 nm. CZP elution was achieved with retention times of 5.6 min and 9.0 min for SEC and RP-LC, respectively.

The mediating role of FoMO and the moderating role of narcissism in the impact of social exclusion on compulsive buying: a cross-cultural study

Abstract Background There is an interrelationship between the concepts of social exclusion, compulsive buying behavior, fear of missing out (FoMO), and narcissism. Nevertheless, the extent to which these concepts mediate or moderate their relationships with each other has not been efficiently investigated. Objective This research aims to investigate how FoMO mediates and narcissism moderates the correlation between social exclusion and compulsive buying behavior. In addition, the research aims to test a conceptual model and highlight the differences that may occur in the conceptual model proposed in two different countries. Methods This model was analyzed among 1007 university students (Turkey =506, Denmark =501). The study used scales to measure social exclusion, compulsive buying behavior, FoMO, and narcissism. The study employed PROCESS Model 4 to analyze direct and indirect (mediation) effects and PROCESS Model 59 to assess conditional (moderation) effects. Furthermore, the Johnson-Neyman technique was utilized to investigate interaction terms. Results The findings indicate that those who face social exclusion tend to participate more in compulsive buying, and this connection is partly explained by FoMO. This suggests that individuals who encounter social exclusion may have an increased likelihood of experiencing FoMO, which may subsequently contribute to compulsive buying behavior. Furthermore, the moderating effect of narcissism differed between the Turkey and Danish samples. Specifically, in the Turkey sample, narcissism only modified the connection between social exclusion and FoMO, while in the Danish sample, it impacted both the connection between social exclusion and FoMO and the connection between FoMO and compulsive buying. Conclusion The obtained results show that the regulating role of narcissism is different in Turkey and Denmark within the conceptual model we studied.

A cidade, palco de tensões e ocupações criativas no espaço: experiências de jovens moradores de periferias / The city, stage of tensions and creative occupations in space: experiences of young residents of the suburbs / La ciudad, escenario de tensiones y ocupaciones creativas en el espacio: experiencias de jóvenes que viven en la periferia

RESUMO Este trabalho buscou pesquisar as relações de um grupo de jovens moradores de regiões periféricas de uma cidade do interior de Minas Gerais com os espaços de casa e da cidade, por meio dos significados e das experiências vivenciadas nesses locais, em interlocução com o referencial teórico do psicodrama. As metodologias utilizadas foram: relato de experiência de um sociodrama realizado antes da pandemia de Covid-19 e, posteriormente, entrevistas semiestruturadas analisadas por meio da teoria fundamentada nos dados. A ocupação dos espaços comunitários de forma criativa, a extensão das relações solidárias no bairro, a praça como espaço da diversidade e também de repressão foram resultados encontrados. O psicodrama pode contribuir como ferramenta significativa para análise dos contextos sociais e espaciais em interlocução com os processos subjetivos de forma crítica, reafirmando seu compromisso social e o direito à cidade.

ABSTRACT This work aimed to investigate the relationships of a group of young people living in suburb regions of a city in the interior of Minas Gerais with social places, based on the meanings and experiences in the home and city environments, in dialogue with the theoretical framework of psychodrama. The methodologies used were experience report of a sociodrama performed before the COVID-19 pandemic and, subsequently, semi-structured interviews analyzed by grounded theory methodology. The occupation of community places in a creative way, the extension of solidarity relationships in the neighborhood, the square as a space of diversity and also of repression were the research findings. Psychodrama can contribute as a significant tool for the analysis of social and spatial contexts in dialogue with subjective processes in a critical way, reaffirming its social commitment and the right to the city.

RESUMEN Este trabajo buscó investigar las relaciones de un grupo de jóvenes que viven en regiones periféricas de una ciudad del interior de Minas Gerais con los espacios del hogar y de la ciudad, a partir de los significados y experiencias en esos ambientes, en diálogo con el referencial teórico del Psicodrama. Las metodologías utilizadas fueron: relato de experiencia de un sociodrama realizado antes de la pandemia de la Covid-19 y, posteriormente, entrevistas semiestructuradas analizadas mediante Teoría Fundamentada en los Datos. La ocupación de los espacios comunitarios de forma creativa, la ampliación de las relaciones solidarias en lo barrio, la plaza como espacio de diversidad y también de represión fueron los resultados encontrados. El psicodrama puede contribuir como una herramienta significativa para el análisis de los contextos sociales y espaciales en diálogo con los procesos subjetivos de una manera crítica, reafirmando su compromiso social y el derecho a la ciudad.

O avesso do mesmo lugar / El revés del mismo lugar / The reverse of the same place / L'envers du même endroit

Neste artigo, parte-se das experiências de fronteira de dois meninos. Uma é a narrativa de Zain, no filme Cafarnaum, anterior à sua migração do Líbano; a outra, o caso clínico de Túlio, construído por um grupo de supervisão em serviço de medidas socioeducativas em São Paulo. Abordaremos o laço social nesses distintos contextos, observando subjetividades singulares e universais de adolescentes marcados por desigualdade e injustiça social. O objetivo é interrogar a psicanálise diante da condição de desenraizamento imposta ao sujeito na contemporaneidade. O cinema, objeto da cultura, evoca a nossa responsabilidade ética ao aproximar ficção e realidade, denunciando violências de gênero e situações desumanas oferecidas aos imigrantes e às infâncias subalternizadas. Há o convite para a criação de dispositivos clínicos que ofereçam a hospitalidade como operador conceitual diante das urgências sociais

El presente artículo se basa en las experiencias fronterizas de dos niños. Una es la narración de Zain en la película Cafarnaum, antes de su emigración del Líbano; la otra es el caso clínico de Tulio, construido por un grupo de supervisión em un servicio de medidas socioeducativas en São Paulo. Abordaremos el vínculo social en estos diferentes contextos, observando subjetividades singulares y universales de adolescentes marcados por la desigualdad y la injusticia social. El objetivo es cuestionar el psicoanálisis ante la condición de desarraigo que se impone al sujeto en la contemporaneidad. El cine, objeto de la cultura, evoca nuestra responsabilidad ética al unir ficción y realidad, denunciando la violencia de género y las situaciones inhumanas que se ofrecen a los inmigrantes y a las infancias marginadas. Hay una invitación a crear dispositivos clínicos que ofrezcan hospitalidad como operador conceptual ante las urgencias sociales

This article is based on the frontier experiences of two boys. One is Zain's narrative in the movie Cafarnaum, before his emigration from Lebanon; the other is the clinical case of Tulio, developed by a supervision group in a social and educational measures service in São Paulo. We will approach the social bond in those different contexts, observing singular and universal subjectivities in adolescents marked by inequality and social injustice. The goal is to interrogate psychoanalysis in the face of the uprooting condition imposed on contemporaneous subjects. Cinema, an object of culture, evokes our ethical responsibility by bringing fiction and reality together, denouncing gender violence and inhuman situations faced by immigrants and underprivileged children. There is an invitation to create clinical devices that offer hospitality as a conceptual operator in the face of social urgencies

Dans cet article, nous partons des expériences frontalières de deux garçons. L'un est le parcours de Zain dans le film Cafarnaum, avant sa migration du Liban ; dans l'autre, le cas clinique de Tulio, construit par un groupe de supervision d'une institution de protection socio-éducative à São Paulo. Nous aborderons le lien social dans ces différents contextes, en observant les subjectivités singulières et universelles d'adolescents marqués par des inégalités et l'injustice sociale. Il s'agit d'interroger la psychanalyse face à la condition de déracinement imposée au sujet dans le contemporain. Le cinéma, objet de culture, évoque notre responsabilité éthique en proposant la rencontre entre fiction et réalité, en dénonçant la violence de genre et les situations inhumaines imposées aux immigrés et aux enfances subalternes. Il s'agit ici d'une invitation à créer des dispositifs cliniques qui partent de l' hospitalité comme opérateur conceptuel face aux urgences sociales

Mitral Surgery for Severe Mitral Annular Calcification: Calcium Resection or Exclusion Procedure / 日本心臓血管外科学会雑誌

Background: Extensive calcification of the mitral annulus (MAC) represents a problem for mitral surgery due to a technical difficulty for implanting prosthetic valves, because the calcium does not allow placing direct sutures through the annulus and para-prosthetic leakage or cardiac rupture may result. We describe our experience with mitral valve surgery with or without decalcification of the annulus. Methods: Since 2005, we performed mitral valve operations in 1,327 patients and among them, severe mitral annular calcification was present in 25 (1.9%). Results: The mean age was 75±9 years and there were 6 men and 19 women, with hemodialysis in 4. Mitral valve replacement (MVR) was performed in all patients. A concomitant operation was aortic valve replacement in 19 and coronary artery bypass grafting in 4 patients. The MAC was resected in 14 (Group-R) and was excluded in 11 (Group-E). In-hospital mortality was four patients in Group-R. The cause of death was congestive heart failure in two, cerebellar infarction in one, and respiratory failure in one. Postoperative morbidity included one patient with reexploration and one with prolonged respiratory failure The aortic cross clamp time was a mean of 180±44 min (range 108∼266 min) in Group-R and 139±32 min (range 61∼186 min) in Group-E (p=0.009). The size of the prosthetic mitral valve was 24.3±1.0 mm in Group-R and 24.6±0.8 mm in Group-E (p=0.618). The postoperative echocardiography showed no abnormal mean pressure gradient of mitral valve prosthesis nor para-valvular leak in any patients in either group. Conclusions: The mitral valve replacement without annular decalcification in a severely calcified mitral annulus is a safe and an effective approach in an elderly patient.

El componente interpersonal de la condición personal: entre las actitudes de reconocimiento y la inclusión de las personas con discapacidades

Propósito/Contexto. Discutir el componente interpersonal de la condición personal, evidenciando cómo las actitudes de reconocimiento determinan la inclusión de las personas con discapacidad. Metodología/Enfoque. Se realizó un análisis teórico sobre el componente interpersonal de la persona humana, a partir del argumento de la inclusión de los individuos con discapacidad y su relevancia para la total participación ciudadana. La investigación se encuadra en el paradigma interpretativo con carácter cualitativo, empleándose como técnica el análisis de documentos considerados fuentes primarias. Asimismo, se asumió el enfoque teórico de Paul Ricoeur respecto a las formas del reconocimiento humano y de las tres formas básicas del reconocimiento interpersonal propuestas por Axel Honneth. Resultados/Hallazgos. La dimensión interpersonal fundamenta nuestra existencia como comunidad humana. Las formas básicas del reconocimiento son recíprocas y, por tanto, no unidireccionales y coinciden con ser respetados, amados y valorados por nuestra contribución a la comunidad. Vivir con personas que no tienen actitudes de reconocimiento hacia uno es una realidad con la cual están familiarizadas las personas con discapacidad. Discusión/Conclusiones/Contribuciones. Para ser plena la inclusión de las personas con discapacidad, además de considerar la modalidad de participación institucional y técnica, se debe tener en cuenta la dimensión interpersonal.

Purpose/Background. Discuss the interpersonal component of the personal condition, showing how recognition attitudes determine the social inclusion of people with disabilities. Methodology/Approach. A theoretical analysis was carried out on the interpersonal component of the human person, based on the argument of the inclusion of people with disabilities and its relevance for full citizen participation. The research is framed in the interpretive paradigm, with a qualitative nature, using as a technique the analysis of documents considered primary sources. Likewise, the theoretical approach of Paul Ricoeur regarding the forms of human recognition and the three basic forms of interpersonal recognition proposed by Axel Honneth were assumed. Results/Findings. The interpersonal dimension is an important foundation of existence as a human community. The basic forms of recognition are reciprocal and, therefore, not unidirectional and coincide with being respected, loved, and valued for our contribution to the community. Living with people who do not have attitudes of recognition towards you is a reality with which people with disabilities are familiar. Discussion/Conclusions/Contributions. To fully include people with disabilities, in addition to considering the modality of institutional and technical participation, it must consider the interpersonal dimension.

Finalidade/Contexto. Discutir a componente interpessoal do estatuto pessoal, mostrando como as atitudes de reconhecimento determinam a inclusão de pessoas com deficiência. Metodologia/Aproximação. Foi realizada uma análise teórica da componente interpessoal da pessoa humana, com base no argumento da inclusão de indivíduos com deficiência e da sua relevância para a plena participação dos cidadãos. A investigação está enquadrada no paradigma interpretativo de carácter qualitativo, utilizando como técnica a análise de documentos considerados como fontes primárias. Do mesmo modo, a abordagem teórica de Paul Ricoeur às formas de reconhecimento humano e as três formas básicas de reconhecimento interpessoal propostas por Axel Honneth foram assumidas. Resultados/Descobertas. A dimensão interpessoal está na base da nossa existência como comunidade humana. As formas básicas de reconhecimento são recíprocas e portanto não unidireccionais e coincidem com serem respeitadas, amadas e valorizadas pela nossa contribuição para a comunidade. Viver com pessoas que não têm atitudes de reconhecimento em relação a uma é uma realidade com a qual as pessoas com deficiência estão familiarizadas. Discussão/Conclusões/Contribuições. Para ser plenamente inclusiva das pessoas com deficiência, para além de considerar o modo institucional e técnico de participação, a dimensão interpessoal deve ser tida em conta.

Corpo feminino e sexualidade: gordura, feiura e exclusão social / Female body and sexuality: fatness, ugliness and social exclusion / Corps féminin et sexualité: graisse, laideur et exclusion sociale

O presente artigo busca investigar o lugar do corpo na atualidade, uma vez que ele nunca é totalmente apreendido pelos dispositivos culturais que possibilitem sua submissão completa às expectativas sociais. Neste sentido, é legítimo afirmar haver certa dificuldade epistêmica, no que tange às disputas de narrativas em torno do corpo gordo. Culturalmente legitimado, observamos um discurso dominante que sugere a responsabilização do sujeito por sua condição corporal. De modo análogo, a criminalização da gordura aumenta quando a sobrepomos à questão de gênero, conforme ilustrarão algumas vinhetas; fruto de entrevistas realizadas anteriormente pela autora, na pesquisa que desenvolve sobre corpo feminino, imagem, sofrimento psíquico e regulação social. A "feiura" ou o "estranho", muitas vezes poderão ser tomados como uma mensagem, cuja força reside, justamente, na sua surpreendente apresentação. Neste sentido, as práticas corporais podem ser compreendidas em um cenário de indiferenciação, na estetização da vida cotidiana, de sujeição ao discurso do outro, mas também, como uma contestação, como problematização do estatuto do corpo na contemporaneidade.

This article seeks to investigate the place of the body nowadays, since it is never fully apprehended by cultural devices that allow its complete submission to social expectations. In this sense, it is legitimate to affirm that there is some epistemic difficulty, regarding the disputes of narratives around the fat body. Culturally legitimized, we observe a dominant discourse that suggests the responsibility of the subject for body condition. Similarly, the criminalization of fat increases when we overwrite it the issue of gender, as some vignettes will illustrate; the result of interviews previously conducted by the author, in the research she develops on the female body, image, psychic suffering and social regulation. The "ugliness" or the "strange" can often be taken as a message, whose strength lies precisely in its surprising presentation. In this sense, body practices can be understood in a scenario of indifferentiation, in the instantiation of everyday life, of subjection to the discourse of the other, but also, as a contestation, as a problematization of the body's status in contemporaneity.

Cet article cherche à étudier la place du corps de nos jours, car il n'est jamais complètement appréhendé par des dispositifs culturels qui permettent sa soumission complète aux attentes sociales. En ce sens, il est légitime d'affirmer qu'il existe une certaine difficulté épistémique, en ce qui concerne les disputes de récits autour du corps gras. Culturellement légitimé, nous observons un discours dominant qui suggère la responsabilité du sujet pour son état corporel. De même, la criminalisation des graisses augmente lorsque nous écrasons la question du genre, comme certaines vignettes l'illustreront; le résultat d'entretiens précédemment menés par l'auteure, dans la recherche qu'elle développe sur le corps féminin, l'image, la souffrance psychique et la régulation sociale. La «laideur¼ ou l'«étrange¼ peut souvent être prise comme un message, dont la force réside précisément dans sa présentation surprenante. En ce sens, les pratiques corporelles peuvent être comprises dans un scénario d'indifférenciation, dans l'estantiation de la vie quotidienne, de soumission au discours de l'autre, mais aussi, comme contestation, comme problématisation du statut du corps dans la contemporanéité.

Precariedade social e exclusão em tempos de Covid-19: um olhar psicanalítico / Social precariousness and exclusion during Covid-19: a psychoanalytic look / Precariedad social y exclusión en tiempos de Covid-19: una mirada psicoanalítica

Neste artigo, é analisada a questão do impacto psicológico produzido pela coronavirus disease 2019 (Covid-19) tendo em vista sua incidência num contexto de exclusão e precariedade social. Trata-se de experiências subjetivas de ruptura de laços sociais e simbólicos que são relativas a diferentes planos de pertencimento coletivo: econômico, político, racial e cultural. Os modos de enfrentamento e a própria ameaça que a pandemia representa são reveladores da sociedade atual considerando-se, particularmente, a singularidade de suas diferentes camadas. A partir de um referencial psicanalítico, com ênfase na articulação subjetividade/laço social, é explorada a ideia de que o fenômeno da Covid-19 produz efeitos de aprofundamento de processos de exclusão, já existentes na cena social contemporânea, com consequências nefastas para o psiquismo de sujeitos cuja existência é marcada pela invisibilidade social. Diante de uma crescente situação de desproteção, acirrada pela crise econômica, política e sanitária, o sujeito luta diariamente pela sobrevivência física, o que pode resultar em uma traumática situação de urgência subjetiva

In this article, we analyze the issue of the psychological impact of the coronavirus disease 2019 (Covid-19) in view of its incidence in a context of exclusion and social precariousness. We refer to subjective experiences of rupture of social and symbolic bonds that are related to different forms of belonging: economic, political, racial, and cultural. The threat that the pandemic represents and the ways of coping with it reveal central aspects of society nowadays, considering the singularity of its different layers. From a psychoanalytical point of view, stressing the articulation of subjectivity/social bond, we study the idea that the phenomenon of Covid-19 intensifies the processes of exclusion, already present in the contemporary social scene, with nefarious consequences for the psyche of subjects whose existence is marked by social invisibility. In the context of a situation of social insecurity, exacerbated by unprecedented economic, political, and health crisis, individuals face a daily struggle for survival, which can result in a traumatic experience of subjective urgency

En este artículo, analizamos el tema del impacto psicológico de la coronavirus disease 2019 (Covid-19) en vista de su incidencia en un contexto de exclusión y precariedad social. Se trata de experiencias subjetivas de ruptura de vínculos sociales y simbólicos que se relacionan con diferentes planes de pertenencia colectiva: económico, político, racial y cultural. Las formas de enfrentamiento y la propia amenaza que representa la pandemia revelan la sociedad actual, teniendo especialmente en cuenta la singularidad de sus diferentes estratos. Desde un punto de vista psicoanalítico, destacando la articulación subjetividad/vinculo social, exploramos la idea de que el fenómeno de Covid-19 produce efectos de profundización de los procesos de exclusión, ya existentes en la escena social contemporánea, con consecuencias nefastas para la psique de sujetos cuya existencia está marcada por la invisibilidad social. Ante una situación creciente de desprotección, agravada por la crisis económica, política y sanitaria, el sujeto enfrenta una lucha diaria por la supervivencia, lo que puede resultar en una experiencia traumática de urgencia subjetiva.

Clinical value of totally laparoscopic exclusion of splenic artery aneurysm combined with pericardial devascularization for portal hypertension complicated with splenic aneurysm / 中华消化外科杂志

Objective:To investigate the clinical value of totally laparoscopic exclusion of splenic artery aneurysm combined with pericardial devascularization for portal hypertension com-plicated with splenic aneurysm.Methods:The retrospective and descriptive study was conducted. The clinicopathological data of 17 patients with portal hypertension complicated with splenic aneurysm who were admitted to 2 medical centers (15 cases in Shenzhen University General Hospital and 2 cases in Wuhan First Hospital) from January 2013 to May 2020 were collected. There were 7 males and 10 females, aged (59±14)years. All patients underwent totally laparoscopic exoclusion of splenic artery aneurysm combined with pericardial devascularization. Observation indicators : (1) surgical and postoperative conditions; (2) complications; (3) follow-up. Follow-up was conducted by out-patient examiantion and telephone interview to detect the effect of exclusion of arterial tumor, and blood re-flow, portal vein thrombosis and survival of patients 3 months after operation. The follow-up was up to December 2020. Measurement data with normal distribution were represented as Mean± SD, and measurement data with skewed distribution were represented as M(range). Results:(1) Surgical and postoperative conditions. All 17 patients successfully completed the operation, without perioperative death. The operation time, volume of intraoperative blood loss of 17 patients were (181±30)minutes, 187(range, 90?420)mL. The white blood cell count, red blood cell count, hemoglobin, serum albumin were (9±4)×10 9/L, (3.5±0.9)×10 12/L, (86±17)g/L, (36±7)g/L on the postoperative day 3. Time to postoperative abdominal drainage tube removal and duration of post-operative hospital stay were (7±4)days and (11±4)days. (2) Complications. All 17 patients had ascites after surgery, which were improved after oral treatment with diuretics. There was no complication such as intra-abdominal hemorrhage, gastrointestinal fistula, pleural effusion, infection, abscess formation, fever and vascular embolism. (3) Follow-up. All the 17 patients were followed up for 28.6(range, 7.0?84.0)months. During the follow-up, the splenic aneurysm cavity of all patients was completely isolated, no blood re-flow and no portal vein thrombosis was observed, and no patient died. Conclusion:Totally laparoscopic exclusion of splenic artery aneurysm combined with pericardial devascularization is safe and feasible in the treatment of portal hypertension complicated with splenic aneurysm.

Expression and refolding of OLA Ⅰ protein with peptides derived from sheeppox virus / 生物工程学报

The aim of this study was to refold the OvisAries leukocyte antigen (OLA) class Ⅰ protein with peptides derived from sheeppox virus (SPPV) to identify SPPV T cell epitopes. Two pairs of primers were designed based on the published sequence of a sheep major histocompatibility complex Ⅰ to amplify the heavy chain gene of OLA Ⅰ α-BSP and the light chain gene of OLA Ⅰ-β2m. Both genes were cloned into a pET-28a(+) expression vector, respectively, and induced with ITPG for protein expression. After purification, the heavy chain and light chain proteins as well as peptides derived from SPPV were refolded at a ratio of 1:1:1 using a gradual dilution method. Molecular exclusion chromatography was used to test whether these peptides bind to the OLA Ⅰ complex. T-cell responses were assessed using freshly isolated PBMCs from immunized sheep through IFN-γ ELISPOT with peptides derived from SPPV protein. The results showed that the cloned heavy chain and light chain expressed sufficiently, with a molecular weight of 36.3 kDa and 16.7 kDa, respectively. The protein separated via a SuperdexTM 200 increase 10/300 GL column was collected and verified by SDS-PAGE after refolding. One SPPV CTL epitope was identified after combined refolding and functional studies based on T-cell epitopes derived from SPPV. An OLA Ⅰ/peptide complex was refolded correctly, which is necessary for the structural characterization. This study may contribute to the development of sheep vaccine based on peptides.