ABSTRACT
OBJECTIVE To investigate the storage time and the origin of bacteria for disinfection package for combat readiness under natural environment in inland and plateau situation.METHODS We prepared double-cotton operation dressing packet,apparatus package,as well as dressing and apparatus mixed packet each with 72 pieces,respectively.Packages were deposited within closed and opened storage boxes under natural environment in depository for combat readiness,respectively after vacuum steam sterilization.Three different kinds of packages each with 3 items were put inside each box.After sterilization,total of 9 items with each 3 from one type of package were taken randomly from each group every time for bacterial culture by different time.At the same time,samples of the air and depot boxes in warehouse for combat readiness were collected and cultured as well as analyzed for bacterial homology.RESULTS In Chongqing,all packages were negative for bacteria on the 40th day after sterilization,while 2 packages were positive for bacteria on the 49th day.In Lhasa,4 packages were positive in opened group on the 106th day after sterilization.On the 122nd day,6 packages were positive in opened group and 1 item was positive in closed group.The dominant bacteria are bacilli and staphylococci both in the air and depot boxes of the warehouse for combat readiness.CONCLUSIONS The storage time for disinfection package under natural environment in different areas correlates closely with the local environmental and climatic situations.The bacteria of the package were confirmed to be from the air.The effective storage time for disinfection package would be prolonged by standardization of the disinfection procedure,and control multiple environmental factors affecting microorganisms.