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Aim and objectives: This study is to determine the prevalence of Extended spectrum beta-lactamases (ESBLs) in urinary isolates. To compare different phenotypic methods for ESBL detection and to evaluate the antibiotic resistance patterns among ESBL producing urinary Escherichia coli. Methodology: rd Urinary isolates of E.coli that were resistant to at least one of 3 generation cephalosporins (cefotaxime, ceftazidime) were screened and tested for ESBLproduction using the Double disc diffusion test (DDDT), quantitative E-strip method and Automated Vitek-2. Result: A total of 341 Klebsiella pneumoniae and Escherichia coli isolates were screened from patients with symptomatic UTI. 207 (60.7%) Escherichia coli, 134 (39.2%) Klebsiella pneumonia. ESBL production was detected in 48.8% and for 161 screening test positive E. coli, ESBL production was detected in 40%
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Recently, a number of Saudi studies have indicated the emergence of a new genetic mutation in gram-negative bacteria (GNB) strains, particularly in extended spectrum beta-lactamase (ESBL) producing isolates, which accounts for about 8% to 38% of the total GNBs detected at Saudi hospitals. ESBLs are enzymes identified in GNB and have ability to resist beta lactam antimicrobial agents by breaking down the lactam ring. To ensure the objectiveness of this study, this paper presents most of the published studies on ESBL infection in Saudi Arabia (available online). ESBL-producing bacteria were detected using disk diffusion methods, dilution methods, double-disc synergy test, E-test strip and molecular detection methods. Risk factors contributing to the spread of ESBL infection include renal disease, diabetes, age, gender, hospital admission and previous exposure to antibiotics. CTX-M, TEM and SHV genotypes are the most common in the studies that have been performed in Saudi hospitals. Imipenem, meropenem, tigecycline and nitrofurantoin are still the best options to treat the ESBL infection. Appropriate infection control policies should be applied to reduce the risk factors of such infections.
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Objective To investigate the clinical manifestations and the antibiotics resistance patterns in children with positive blood culture of Escherichia coli. Methods The clinical data of children with positive blood culture of Escherichia coli were retrospectively analyzed from Jan.2007 to Dec.2014. Results In a total of 154774 children who had blood culture in the study period, 8446 children were positive, among whom 408 (4.83%) children were isolated Escherichia coli. The children with the positive blood culture of Escherichia coli mainly were under one year old (51.72%), of which 36.77%was neonates. There were 275 children had underlying diseases, and the most common disease was Leukemia. 199 (48.77%) Escherichia coli strains were producing extended spectrumβ-lactamase (ESBLs) and 85.23%were resistant to ampicillin. All strains were susceptible to meropenem. Conclusions Septicemia caused by Escherichia coli is usually occurred in children with leukemia or in neonates. Since blood infections of Escherichia coil had high rate of ESBLs, the use of carbapenem antibiotics should be cautious.
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OBJECTIVE To study the risk factors of extended-spectrum beta-lactamases(ESBLs) producing bacteria infection in children,and provide reference to prevent and control the prevalence of bacterial strain of ESBLs.METHODS In a case and control studys the samples were selected randomly from 2007 to 2009 in the Children′s Hospital of Shanxi Province.The samples of case and control were all 100.RESULTS ?2 Test showed that boy and baby,previous history,pneumonia,medical ward,hospital infection and using antibiotics before admission to hospital were the risk factors;t-test showed that high white blood cell and neutrophil were the protective factors;Logistic regression showed that boy,previous history,hospital infection,using antibiotics before admission to hospital and medical ward were the risk factors and anal tube was a protective factor.CONCLUSIONS Increasing the rate of bacteriological test to the children who have the relative risk factors is very important to prevent and control the prevalence of ESBLs strain.
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OBJECTIVE To type the genes of plasmid DNA in 54 clinical Klebsiella pneumoniae isolates producing extended -spectrum beta-lactamases (SHV) by denaturing high-performance liquid chromatography (DHPLc) and evaluate their sensitivity and specificity, and explore a rapid and convenient method for detecting the antibiotic resistance of K. pneumoniae. METHODS Plasmid DNA from each extended-spectrum beta-lactamase (SHV) producing strain was subjected to PCR amplification. After we performed DNA sequencing of these amplicons and identification of mutation and their genotype, DHPLc was undertaken to investigate whether its results correlate the distinctive chromatogram with each genotype. RESULTS All the strains were found abnormal elution peaks (two or three peaks) which were different from each other. The result of DNA sequencing demonstrated that all the strains had DNA mutation in comparison with SHV-1. Moreover, DHPLc could produce specific peak patterns that correlate with genotype. CONCLUSIONS The sensitivity of DHPLc is 100% in this study. And each genotype is corresponded to specific peak pattern. So we can use DHPLc technique to type the genes of plasmid DNA in K. pneumoniae and detect mutations rapidly. DHPLc not only has high accuracy , but also is a convenient and rapid technique for the detection of mutation in the bacterial genome. It has a great potential clinical value.
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0.05).Except imipenem and ampicillin,there was significant difference between the resistance of non-ESBLs-producing strains from CAP and from HAP to other eleven kinds of antibiotics(?2 test,P