ABSTRACT
Calotropis procera (Ait.) R. Br (Asclepiadaceae) is a species widely used in traditional medicine for the treatment of various diseases such as sickle cell disease, asthma and cancer. In Burkina Faso, it enter in the composition of FACA® in combination with Zanthoxylum zanthoxyloides Lam (Rutaceae), drug used in sickle cell disease treatment. The objective of this study was to evaluate the in vitro cytotoxicity of aqueous extract of root barks of the plant on cell lines to increase the safe use of FACA®. MTT and Neutral Red assays performed on Caco-2 and Neuro-2a cell lines revealed that aqueous extract from root barks of Calotropis procera are cytotoxic on these cell lines. DNA fragmentation assay on Caco-2 cell showed DNA smearing reflecting a degradation of nuclear material that indicates a possible genotoxicity. Altogether, it comes out that the most sensitive cell line is the human colorectal carcinoma Caco-2 cells. Comparatively the active compounds of Calotropis procera do not affect the mice nervous system cells in the same dramatic extent. Our results strongly suggest that patients under treatment of FACA® must respect doses prescribed in order to avoid adverse side effects on the gastrointestinal tract.
ABSTRACT
Ferimentos a faca cravados na região maxilofacial são raros e pouco relatados na literatura. A importância do conhecimento acerca desses incidentes está no risco de vida ao paciente, especialmente nos casos em que envolvem estruturas cranianas nobres, grandes vasos sanguíneos e obstrução das vias aéreas por hemorragia. Será relatado o caso de um paciente do gênero masculino de 22 anos que foi ferido em uma briga e deu entrada no hospital com uma faca de cozinha cravada na região do nariz e que tinha comunicação com a cavidade bucal...
Impacted knife injuries in the maxillofacial region are uncommon and scarcely reported in the literature. Knowledge regarding knife-inflicted trauma is of paramount importance because these injuries are life-threatening, specially in cases involving vital cranial structures, large blood vessels and airway obstruction caused by hemorrhage. Here we report the case of a 22-year-old male who sustainted a stab wound during a fight and ways admitted to hospital with the blade of a kitchen knife lodged in the lateral region of the nose communicating with the oral cavity...