ABSTRACT
[Objective] To investigate the far-upstream element (FUSE) binding protein 1 (FUBP1) expression in tongue squamous cell carcinoma (TSCC) and its adjacent normal tissues,to explore the role of FUBP1 in the initiation and development of TSCC and its clinical significance.[Methods] FUBP1 expression in TSCC tissues and its adjacent normal tissues was detected by immunohistochemistry,qRT-PCR and Western blot respectively.Statistical analysis was used to analyze the relationship between FUBP1 expression and clinical pathological parameters and prognosis.[Results] FUBP1 expression in TSCC tissues was higher than that in the corresponding adjacent normal tissues (P＜0.05);FUBP1 expression in TSCC tissues was higher in patients of advanced stage and with cervical lymph node metastasis (P＜0.001);The 5-year survival rate of FUBP1 over expression group was lower than that of FUBP1 low expression group (P=0.035);FUBP1 may be an independent predictor in patients with TSCC.[Conclusion] The high expression of FUBP1 in TSCC tissues is associated with poor prognosis in patients with TSCC,therefore,high expression of FUBP1 may promote the formation and development of TSCC,and become a index for prognosis in TSCC patients.
ABSTRACT
Objective To investigate the expression of far upstream element binding protein 1 (FUBP1) gene in gastric cancer,and analyze its effect on proliferation and invasion of gastricc cancer cells.Methods The expression of FUBP1 in gastric cancer tissues was detected by real-time PCR.The relationship between FUBP1 expression and clinic pathological factors of gastricc cancer was analyzed by one-way ANOVA.The silence vector for FUBP1 was constructed and transfected into gastricc cancer SGC7901 cells,and the transfected effects were then detected by real-time PCR.The influence of FUBP1 silence on cell proliferation and invasion in SGC7901 cells were detected by CCK8 method and Transwell assay.Results FUBP1 expression was increased markedly in gastricc cancer tissues.Following the depressing of differentiation and the increase of invasion depth and metastatic lymph nodes,the FUBP1 expression was up-regulated in primary gastric carcinoma significantly.The pS-FUBP1 vector could silence the FUBP1 expression in SGC7901 cells.FUBP1 silence inhibited SGC7901 cell proliferation amd invasion.Conclusion FUBP1 was high-expressed in gastricc cancer,and related with genesis and progress of gastric cancer,silence of FUBP1 expression can inhibit the cell proliferation and invasion in gastric cancer cells.