ABSTRACT
Objective: To study the chemical constituents from the aerial parts of Fagopyrum dibotrys. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Results: Fourteen known compounds were isolated in the methanol extract from the aerial parts of F. dibotrys and their structures were identified as benzoic acid (1), p-hydroxybenzoic acid (2), p-hydroxy benzaldehyde (3), 3,4-dihydroxy benzoic acid (4), succinic acid (5), caffeic acid (6), methyl caffeate acid (7), luteolin (8), tricin (9), quercetin (10), afzelin A (11), 2α,3β,29-trihydroxyolean-12-en-28-oic acid (12), yarumic acid (13), and 3α-hydroxy-urs-12,15-dien (14). Conclusion: Compounds 2-3 and 6-9 are firstly obtained from the aerial parts of F. dibotrys. Compounds 11-14 are isolated from the genus of Fagopyrum Mill. for the first time.
ABSTRACT
Objective: To study the chemical constituents from the root tubers of Fagopyrum dibotrys. Methods: The compounds were isolated and purified by means of chromatographic techniques and their structures were identified on the basis of spectral features. Results: Fourteen known compounds were isolated from methanol extract in the dry roots of F. dibotrys and thier structures were identified as luteolin (1), tricin (2), luteolin-7,4'-dimethyl ether (3), quercetin (4), genkwanin (5), chrysoeriol (6), protocatechuic acid (7), protocatechuic acid methyl ester (8), p-hydroxybenzaldehyde (9), glutinone (10), glutinol (11), olean-12-ene-3β, 7β, 15α, 28-tetraol (12), juglangenin A (13), and 21β-dihydroxy-olean-12-ene (14). Conclusion: Compounds 5 and 6 are firstly obtained from F. dibotrys. Compounds 12-14 are isolated from the plants of Fagopyrum Mill. for the first time.
ABSTRACT
Objective: To obtain the full-length cDNA sequence of anthocyanins synthase (ANS) gene from Fagopyrum dibotrys (FdANS), and to analyze the expression of FdANS in each tissue and the total anthocyanin content during florescence of F. dibotrys. Methods: The cDNA sequence of ANS gene was cloned by homology cloning from F. dibotrys. The expression of ANS was analyzed by semi-quantitative RT-PCR. The content of total anthocyanin was measured by spectrophotometry. Results: The open reading frame (ORF) of FdANS was 1077 bp and encoded a protein with 358 amino acids named FdANS. Bioinforamtion analysis suggested that the amino acid sequence of FdANS had the higher homology with those in other plants. Both the gene expression FdANS in different tissues during florescence of F. dibotrys and the total anthocyanin content were in the order of flowers > leaves > stems> roots. Conclusion: The cDNA sequence of FdANS is firstly obtained from F. dibotrys and its coding protein has the typical characteristic of ANS homologous protein. The gene expression of FdANS shows the correlation to the total anthocyanins content in the flowers, leaves, stems, and roots of F. dibotrys.
ABSTRACT
Objective: To clone and analyze the full-length cDNA of chalcone isomerase (CHI) gene from Fagopyrum dibotrys (FdCHI). To analyze the expression of CHI and the total flavonoids content during florescence of F. dibotrys. Methods: The cDNA sequence of CHI was cloned by homology cloning from F. dibotrys. The expression of CHI was analyzed in the different tissues during florescence by semi-quantitative RT-PCR. The content of total flavonoids was measured by AlCl3 method. Results: The open reading frame (ORF) of FdCHI was 750 bp and encoded a protein with 249 amino acids. Bioinforamtion analysis suggested that the amino acid sequence of FdCHI had the high homology with those in other plants. Gene expression analysis showed that FdCHI was highly expressed in the flowers, followed by the roots and leaves, while lower in the stems. The content of total flavonoids was the highest in the flowers then the leaves and stems, and the lowest was in the roots. Conclusion: The cDNA sequence of FdCHI is firstly obtained from F. dibotrys and its coding protein has the typical characteristic of CHI homologous protein. The gene expression of FdCHI shows the same to the total flavonoids content in the stems, leaves, and flowers, but different in the roots of F. dibotrys.