ABSTRACT
OBJECTIVE： To compare the difference in volatile oil constituents of Glehniae littoralis from 3 producing areas as Shandong Laiyang， Hebei Anguo， Inner Mongolian Chifeng. METHODS： The method of steam distillation was used to extract the volatile oil of G. littoralis from different areas and calculate the extraction rate. The constituents of volatile oil were analyzed by using GC-MS. The data was corrected by Xcalibur chemical workstation. The constituents were searched by NIST 11.0 mass spectrometry database （matching degree ＞800）， and the relative mass fraction of each chemical constituent was obtained by peak area normalization. RESULTS： The extraction rate of volatile oil in G. littoralis from Laiyang was 0.013％， which was far lower than G. littoralis from Anguo （0.099％） and G. littoralis from Chifeng （0.105％）. There were 15， 18 and 27 constituents identified in volatile oil of G. littoralis from 3 producing areas； the relative mass fractions were 89.29％， 96.76％， 94.53％. Falcarinol was a common compound with the highest relative mass fraction of the volatile oil of G. littoralis from different producing areas； the relative mass fractions were 69.79％， 90.89％ and 71.04％， respectively. Fatty acids were rich in the sample from Laiyang， while C15H24 sesquiterpenoids were rich in the other samples from Anguo and Chifeng. CONCLUSIONS： Volatile oil of G. littoralis could be used as potential chemical markers to distinguish different producing areas due to their significant differences in chemical components.
ABSTRACT
Objective To identify the chemical constituents of volatile oil from the Chinese traditional medicine coastal glehnia root and to compare the differences between coastal glehnia root of different locations. Methods A total of 16 batches of the coastal glehnia root were collected from several major production areas from September 2012 to March 2013, and then they were ground into powder. The volatile oil was extracted from the powder samples using the methods described in Chinese Pharmacopoeia Appendix. Gas chromatography-mass spectometry (GC-MS) was used to get the spectra of volatile oil of each sample and NIST 11. 0 database was used to identify the chemical constituents of coastal glehnia root. Results and Conclusion From 16 batches of 48 coastal glehnia root volatile oil samples, we identified 12 common components. The 12 common chemical constituents can serve as the characteristic composition of volatile oil of the coastal glehnia root, and falcarinol is the major main chemical constituent. The three batches collected from Hebei province had fewer chemical components and lower contents. We also found that the coastal glehnia root samples with root bark had more volatile oil components and higher contents than the samples without bark; moreover, eicosapentaenoic acid was only found in the samples with root bark. Peeling the bark may reduce the contents of some volatile oils such as eicosapentaenoic acid, which may affect the medicinal activity of the coastal glehnia root.