Production of extracellular lipases by Rhizopus oligosporus in a stirred fermentor

The present investigation deals with the kinetics of submerged extracellular lipases fermentation by both wild and mutant strains of Rhizopus oligosporus var. microsporus in a laboratory scale stirred fermentor. Other parameters studied were inoculum size, pH, agitation and rate of aeration. It was found that the growth and lipases production was increased gradually and reached its maximum 9.07± 0.42ª U mL-1 (W) and 42.49 ± 3.91ª U mL-1 (M) after 30h of fermentation for both wild and mutant strain. There is overall increase of 109 percent (W) and 124 percent (M) in the production of extracellular lipases as compared to shake flask. Another significant finding of the present study is that the fermentation period is reduced to 30 h in case of wild and 23 h in case of mutant from 48 h in shake flask studies. The specific productivity of mutant strain (qp = 377.3 U/g cells/h) was several folds higher than wild strain. The specific production rate and growth coefficient revealed the hyperproducibility of extracellular lipases using mutant IIB-63NTG-7.

Yolanda Irureta de Milá de la Roca Creadora del Fermentador "Caracas" Valioso aporte en la lucha contra el tétano en Venezuela

Yolanda Irureta Story de Milá de la Roca nació el 9 de enero de 1923 en Puerto España (Trinidad). Ocupó diversos cargos en el Instituto Nacional de Higiene, en la División de Elaboración de Productos Biológicos, en las secciones de Vacuna Antivariólica y Toxoide Tetánico, así como en el Departamento de Vacunas Bacterianas, ocupando el cargo de Microbiólogo Jefe hasta su jubilación en 1983. En la década de los setenta, Yolanda Irureta y su equipo asumen el reto de aumentar la producción de Toxoide Tetánico para satisfacer la creciente demanda de este producto, por parte del Ministerio de Sanidad y Asistencia Social (MSAS). Después de 18 meses de trabajo creativo lograron desarrollar un equipo bautizado como Fermentador "Caracas", el cual permitió triplicar la producción de Toxoide Tetánico. El aspecto más resaltante de esta adaptación radica en su sencillez y facilidad de fabricación, el bajo costo de sus componentes y la facilidad de mantenimiento. En aquella ocasión, el Presidente de la República donó el exceso de producción a Costa Rica, Honduras y Bolivia. En 1977, los expertos de la Organización Mundial de la Salud consideraron al laboratorio de Toxoide Tetánico del Instituto Nacional de Higiene "Rafael Rangel" (INH "RR") como el mejor de América Latina. Debido a su valiosa contribución a la tecnología, el CONICIT les otorgó el Premio de Desarrollo Tecnológico, en el año 1979. El 9 de marzo de 1982, el Fermentador "Caracas" logra obtener la patente número 4.318.992, en USA, asegurando así que el INH "RR" conserve los derechos legales sobre esta tecnología, y se reconozca la propiedad intelectual a sus verdaderos autores.

Yolanda Irureta Story de Milá de la Roca was born on January 9, 1923 in Port of Spain, Trinidad. She joined the National Institute of Hygiene (NIH), as a staff member of the Division that manufactures human vaccines. Through the years she was promoted to various positions in the Smallpox Vaccine and Tetanus Toxoid Units, being Head of the Department of Bacterial Vaccines until her retirement in 1983. During the seventies, the Ministry of Health and Social Assistance increased their demand of Tetanus Toxoid. To fulfill this need, Yolanda Irureta and her team faced this challenge, working 18 months to develop a fermentor that allowed them to triplicate the production. The fermentor was named "Caracas" Fermentor and it has many important features. It is simple and economic; it is made up of parts that are easy to obtain and the maintainance cost are very low. At that time, the Venezuelan President donated the surplus of production to Costa Rica, Honduras and Bolivia. In 1977, experts of the World Health Organization considered the Tetanus Toxoid laboratory of National Institute of Hygiene "Rafael Rangel" (NIH"RR"), the best in Latin America. Due to their valuable contribution to technology, they received the 1979 CONICIT Award for Technological Development. On March 9, 1982, it was granted to the Caracas Fermentor, the U.S.A. patent number 4.318.992, securing to the NIH"RR" the exclusive rights to this technology and the intellectual property to theirs authors.

Ferment Engineering and Validation Ideas / 中国生物工程杂志

Fermentation is a technology,validation is a management.Technology and management could not be seperated trom each other.The basic theory of the validation was introduced,the idea of the validation was fused into the ferment technology.The idea of the validation of the ferment system was set forth the whole process of the validation of the ferment system from personal qualification,operation procotol,validation implement,and sum-up reports.It was proved that implementing validation and constructing validation system throughout ferment processing is indispensable for completing a successful fermentation.the validated fermentation system is just a dependable system,the validated ferment technique is a stable technique.

Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test / 中国生物工程杂志

The protoplasts of Micromonospora purpurea,the high yield strains of gentammicin producer were mutagenized by diethyl sulfate(DES)and ultraviolet radiation(UV)respectively,then fused,screened by gentamicin resistance and regenerated.The average fermentation unit 2200?U/ml could be achieved by shake flask for 10 batches.The average fermentation unit 1900?U/ml could be obtained by 5L fermentor for 7 batches.The quality of the end product conformed to CP2000,BP2000 and USP26 pharmacopoeia.