ABSTRACT
OBJETIVOS: Propor um modelo experimental de transplante de células do sistema nervoso fetal de ratos Wistar para o sítio de lesão medular de ratos adultos que permitisse sua sobrevivência e integração para possibilitar protocolos de pesquisa que identificarão outros fatores de regeneração e recuperação funcional pós trauma raquimedular. MÉTODOS: Vinte ratos adultos foram submetidos a laminectomia, e lesão de 5mm de hemimedula realizada com auxílio de microscópio óptico. Quinze deste ratos tiveram seu sítio de lesão medular transplantado com células do sistema nervoso central de fetos de rato; os ratos foram monitorados por 2 dias e tiveram sua coluna vertebral extraída para análise histológica. RESULTADOS: Evidenciou-se que em 60 por cento dos casos as células transplantadas permaneciam viáveis no sítio da lesão e que a reação inflamatória no grupo transplantado era sempre maior que no grupo controle. CONCLUSÃO: O presente trabalho demonstrou a possibilidade de contar com o modelo de pesquisa para transplante de células fetais que permanecem viáveis 2 dias após seu implante.
OBJECTIVE: To propose an experimental model for transplantation of fetal cells from the nervous system of Wistar rats to the site of spinal cord injury in adult rats, to enable their survival and integration for research protocols that identify other factors of regeneration and functional recovery following spinal cord trauma. METHODS: Twenty adult rats were submitted to laminectomy and a 5mm incision was made, using an optical microscope, In fifteen of these rats, the site of the spinal cord lesion was transplanted with cells from the fetal rat central nervous system; the rats were monitored for two days, then the spinal cord was removed for histological analysis. RESULTS: In 60 percent of cases, the transplanted cells remained viable in the site of the lesion; the inflammatory response in the transplanted group was always greater than in the control group. CONCLUSION: This study demonstrates the potential use of this research model for use in the transplantation of fetal cells that remain viable two days after their implantation.
Subject(s)
Animals , Male , Female , Rats , Feasibility Studies , Fetal Stem Cells , Spinal Cord Injuries , Stem Cell Transplantation , Laminectomy/methods , Rats, WistarABSTRACT
The isolation of fetal cells from maternal circulation has the potential to allow relativelyself prenatal diagosis for all pregnant women. The present technology, however, has notreached the accuracy required for clinical diagnosis because of maternal cell contaminationSo we published a new method for enrichment of nRBC in a fetal cell isolation(1996).In this study, attempted to FISH analysis of nRBC which was isolated by our ownmethods. We evaluated the efficiency of FISH.As the results, we have successfully used FISH on enriched nRBC.We were able to identified 2 abnormal fetus which were confirmed by conventionalcytogenentic study as Down syndrome(Fig.1) and Klinefeltre syndrome(Fig.2). And thesensitivity and specificity for FISH was 86%(49/57) and 92.3%(36/39), respectively.According to our results, fetal cell analysis by FISH can be reliable used for prenatalaneuploidy diagnosis. However, the problems of enrichment of the fetal cell and FISH probeor condition should be over come before analyze.
Subject(s)
Female , Humans , Aneuploidy , Diagnosis , Erythroblasts , Fetus , Fluorescence , Pregnant Women , Sensitivity and SpecificityABSTRACT
According to that Y-chromosomal sequence in characterised by Y-specific repeat DNA family (DYZ1 ), which contain 800-5000 copies, a pair of primers Y3, Y4 is designed to amplify their 446bp long of specific DNA segment by polymerase chain reaction (PCR), so as to detect male fetal cells in materal blood. In this paper male fetal cells in maternal blood can be detected by PCR amplification of unpurified DNA from maternal peripheral blood during various stages of gestation (early, middle, late). Compared with villi, ammotic fluid and deliverd neonate sex their coincident rate are 93%. 100%, 87. 5% respectively among three periods. It is revealed that noninvasive examining fetal cells from peripheral blood of pregnant women for diagnosis of sex-linked inherited diseases is significant valuable.