ABSTRACT
【Objective】 To explore the effect of different extraction methods on the content of total flavones from propolis.【Methods】With rutin as the control,spectrophotometry was used to investigate the content of total flavones extracted from propolis.【Results】The content of total flavones extracted by pharmacopoeia extraction method was 0.1982mg/g and 0.1978mg/g by methanol Soxhlet extraction method,the difference being insignificant.【Conclusion】Methanol Soxhlet extraction method is simple,effective and practical,and can be used to replace pharmacopoeia extraction method for the determination of total flavones from propolis when necessary.
ABSTRACT
[Objective] To assess the quality of Citrus grandis Osbeck. Var. tomentosa Hort. (CGO Var. TH) and Citrus grandis (L. ) Osbeck (CGO). [Methods] The macroscopic feature and microscopic structure of CGO Var. TH and CGO were compared. Their content of total flavones (TF) was determined by spectrometry, naringin content and fingerprint by high performance liquid chromatography (HPLC) and the content of rhoifolin by thin-layer chromatography (TLC). Their expectorart, anti-inflammatory and antitussive effects were observed by phenol red test, auricular swelling test and ammonia-water-induced cough method. [Results] Obvious differences such as villi in the exocarp and non-grandular hair in the stem existed in the macroscopic feature and microscopic structure of CGO Var. TH and CGO. The contents o{ total flavones and naringin in CGO Var. TH were markedly higher than that in CGO and the rhoifolin content in CGO Var. TH was ten times as much as that in CGO. Markedly difference was also found in their fingerprint. The expectorant, anti-inflammatory and antitussive effects in CGO Var. TH were better than those in CGO. [Conclusion] The quality of CGO Var. TH is much better than that of CGO.
ABSTRACT
Alcohol-infusion. Conclusion: With the cell-wall-broken extraction process, a higher content of total flavones was obtained from Pollen Typhae .
ABSTRACT
Objective To determine the appropriate collecting -time of Exocarpium citri grandis,the chemical compo-sition in Exocarpium citri grandis in different fruit -ages was compare d and analyzed.Methods Contents of naringein and bergapten were compared by TLC an d the total dihydroflavones in Exocarpium citri grandis was determined by spec-trophotometmic analysis with detection wavelength at 384nm.Results With the increase of fruit -age,the c ontents of naringein and total dihydroflavones in Exocarpium citri grandis decreased and the content of bergapt en increased.Con-clusion Fruit -age has an obviously effect on the chemical composition in Exocarpium citri grandis and collecting -time can influence the quality of medicin e materials.Further studies of Exocarpium citri grandis should be combined pharma-codynamics for fixing collecting -t ime.