Displasia Cemento Ósea Florida: Reporte de un caso

La Displasia Cemento Ósea Florida corresponde a un tipo de lesión fibro ósea caracterizada por presentar un hueso trabecular con apariencia de tejido conectivo fibroso con áreas similares al cemento radicular. Afecta principalmente a mandíbula, a mujeres y a personas de etnia africana entre la cuarta a la quinta década. Posee crecimiento limitado, presentación de forma simétrica, bilateral y capacidad de afectar de uno a más cuadrantes. Tiene tres etapas de desarrollo, que se presentan con aspecto radiográfico diferente. En la primera etapa se aprecia un área radiolúcida, en la segunda etapa se visualizan radiopacidades circunscritas al área radiolúcida; y en la tercera etapa se observa una clara radiopacidad alrededor de la lesión. Para su correcto diagnóstico se necesita una cuidadosa correlación de los hallazgos clínicos, imagenológicos, de laboratorio e histopatológicos. Se presenta el caso de una paciente de género femenino de 70 años diagnosticada con Displasia Cemento Ósea Florida.

Florid Cemento-Osseous Dysplasia is a type of fibro-osseous lesion characterized by a trabecular bone with the appearance of fibrous connective tissue with areas similar to root cement. It mainly affects the jaw, women and people of African ethnicity between the fourth and fifth decades. It has limited growth, a symmetrical, bilateral presentation and the ability to affect one or more quadrants. It has three stages of development, with different radiographic appearance. The first stage shows a radiolucent area, the second stage shows radiopacities circumscribed to the radiolucent area; and in the third stage a clear radiopacity is observed around the lesion. For its correct diagnosis, a careful correlation of clinical, imaging, laboratory and histopathological findings is needed. The case of a 70-year-old female patient diagnosed with Florid Cemento-Osseous Dysplasia is presented.

Correção de sorriso gengival em paciente com displasia cemento óssea florida / Correction of gingival smile in patient with cemento bone dysplasia florida

Com os avanços tecnológicos na odontologia o planejamento digital se tornou algo mais presente na prática clinica. O objetivo desse trabalho é relatar uma experiência vivida em clínica sobre harmonização do sorriso através de uma gengivoplastia e remodelação óssea em paciente com displasia cemento óssea florida. Paciente 42 anos, autodeclarada do sexo feminino, não relatou nenhuma alteração sistêmica, uso de medicamento ou tabagismo, entretanto relatou ter displasia cemento óssea florida com diagnóstico de periodonto saudável. Compareceu a clínica escola de uma instituição de Ensino Superior para realização de tratamento odontológico queixando-se da aparência de seus dentes. Neste caso, foi utilizado escaneamento digital, tomografia computadorizada para tecidos moles, Digital Smile Design (DSD) e Perioguide (guia cirúrgico) para a realização do procedimento de harmonização do sorriso através da cirurgia periodontal. Em suma, apesar da displasia óssea ser um fator limitante ao sucesso ideal do procedimento, o resultado final alcançado foi satisfatório sob ótica do paciente e profissional não havendo recidiva(AU)

With technological advances in dentistry, digital planning has become something more present in clinical practice. The objective of this work is to report a clinical experience on smile harmonization through gingivoplasty and bone remodeling in a patient with florid cemento-osseous dysplasia. Patient 42 years old, self-reported female, did not report any systemic changes, use of medication or smoking, however she reported having florid cemento-osseous dysplasia with a diagnosis of healthy periodontium. He attended the school clinic of a Higher Education institution for dental treatment, complaining about the appearance of his teeth. In this case, digital scanning, soft tissue computed tomography, Digital Smile Design (DSD) and Perioguide (surgical guide) were used to perform the smile harmonization procedure through periodontal surgery. In short, despite bone dysplasia being a limiting factor for the ideal success of the procedure, the final result achieved was satisfactory from the perspective of the patient and professional, with no recurrence(AU)

In vitro ?-amylase and ?-glucosidase inhibitory potential of Pleurotus ostreatus cv. Florida extract

Pleurotus ostreatus cv. Florida is one of the widely used edible mushroom. The polysaccharides from this mushrooms have been studied for antidiabetic potential; however, no efforts have been made to explore the potential of this mushroom to influence carbohydrate metabolizing enzymes viz. ?-amylase and ?-glucosidase. The present work was undertaken to investigate the inhibitory potential of Pleurotus ostreatus cv. Florida on enzymes ?-amylase and ?-glucosidase. Several concentrations of extracts were used to study inhibition of enzymatic activity of ?-amylase and ?-glucosidase. A dose dependent inhibitory effect on enzymes was observed. The current study, for the first time, uncovered ?-amylase and ?-glucosidase inhibitory potential of Pleurotus ostreatus cv. Florida. The study could be helpful to isolate and characterize compounds responsible for it.

GC-MS analysis of volatile oil from traditional She medicine Clematis Florida var. plena flower / 中草药

Objective: Clematis florida var. plena is a traditional She medicine. In this paper, we aim to study the main chemical components of essential oil of C. florida var. plena flower. The study will provide reference for large-scale reasonable cultivation and quality standard establishment of C. florida var. plena. Methods: C. florida var. plena flower's essential oil was extracted with steam distillation and it was identified by GC-MS. Area normalization method was used to measure the percentage of each components. Results: In the experiment, 32 element were detected in essential oil of C. florida var. plena flower, among which 20 chemical components were identified. There are 13 chemical components over 1%, and the top five compounds are palmitic acid (26.94%), phytol (10.58%), linoleic acid (6.13%), pentadecane (4.54%) and n-tricosane (3.84%). Conclusion: GC-MS was first used to analyze the main chemical components of C. florida var. plena's flower essential oil. The study provides scientific basis for cultivation, production, development, utilization of C. florida var. plena.

DISPLASIA ÓSSEA FLORIDA EXPANSIVA: RELATOS DE DOIS CASOS INCOMUNS / EXPANSIVE FLORID OSSEOUS DYSPLASIA: REPORTS OF TWO UNUSUAL CASES

A displasia óssea geralmente é um achado radiográfico, por não apresentar, em sua maioria, alterações clínicas. Contudo, um novo subtipo de displasia, que afeta preferencialmente mulheres negras de meia-idade e promove expansão do osso cortical envolvido, tem sido relatada como Displasia Óssea Expansiva. O objetivo deste artigo é relatar dois casos de Displasia Óssea Florida com expressivo aspecto expansivo e perfuração das corticais ósseas envolvidas. O primeiro relata uma paciente negra, 46 anos, que compareceu à Odontoclínica Central da Marinha necessitando de restauração indireta em um dente. O exame clínico demonstrou um aumento de volume duro na face lingual do rebordo alveolar dos incisivos inferiores. Na radiografia panorâmica, foram observadas duas imagens de densidades mistas com predomínio de áreas radiopacas nas regiões apicais dos dentes anteroinferiores e do primeiro molar inferior esquerdo. A tomografia computadorizada mostrou expansão, adelgaçamento da cortical vestibular e perfuração da cortical lingual na região dos incisivos. O segundo caso apresenta uma paciente de meia idade, feoderma, que buscou atendimento para acompanhamento de lesão mista na mandíbula, nas regiões apicais dos dentes anteriores, pré-molares e na região correspondente ao terceiro molar direito. Na tomografia computadorizada por feixe cônico observaram-se expansão e adelgaçamento da cortical vestibular nos dentes anteroinferiores. Com base na literatura atual e nas características de ambas as lesões, a hipótese diagnóstica para ambos os casos foi de Displasia Óssea Florida com aspecto expansivo. As pacientes foram orientadas sobre a importância da higiene bucal adequada e da necessidade de um acompanhamento clínico imagiológico anual destas lesões.

Osseous dysplasia generally is a radiographic finding because, for the most part, it does not cause clinical changes. However, a new subtype of dysplasia that affects primarily middle-aged black women and promotes expansion of the involved cortical bone has been reported as Expansive Osseous Dysplasia. The aim of this article is to report two cases of Florida Osseous Dysplasia with expressive expansion and perforation of the osseous cortex involved. The first case reports a 46-year-old black female patient whom was attended at the Naval Dental Center requiring indirect restoration in one tooth. Clinical examination showed a hard and increased volume at lingual portion of alveolar ridge in the region of the lower incisors. In the panoramic radiography two mixed density images were observed with predominance of radiopaque areas in the apical region of the anterior inferior teeth and in the first lower left molar. The computed tomography showed the buccal cortex thinning and the expansion and perforation of the lingual cortex in the incisor region. The second case presents a middle-aged brown skin patient who sought dental care to monitor a mixed lesion in the apical region of the anterior inferior teeth, lower premolars on both sides and in the third right lower third molar. In cone beam computed tomography, expansion and thinning of the vestibular cortex in the anterior inferior teeth were observed. Based on the current literature and the characteristics of both lesions, the diagnostic hypothesis was Florida Osseous Dysplasia with expansive aspect. The patients were advised on the importance of oral hygiene and the need for an annual follow-up of these lesions

Conservative Treatment of Chronic Osteomyelitis Associated with Florid Osseous Dysplasia / Tratamiento Conservador de la Osteomielitis Crónica Asociada con Displasia Ósea Florida

ABSTRACT: Florid osseous dysplasia (FOD) is an extensive form of osseous dysplasia where normal trabecular bone is substituted by fibrous connective tissue and amorphous mineralized tissue. Usually, the lesions are mainly asymptomatic and the patients should be followed with clinical and imaging examination, requiring no intervention. Nevertheless, due to the poor vascularization of the lesion and to local trauma, secondary infections and osteomyelitis may occur. Patients may present with pain, mucosal ulceration, lesion exposure in the oral cavity, fistula and swelling. In such cases, the correct diagnosis and management of the lesion is decisive to reestablish patient's health and quality of life. The aim of this article is to present a case of complicating secondary chronic osteomyelitis treated successfully with conservative intervention. A 68-year-old black female patient reported a "swelling of the gums" that was present for eleven years, with episodes of periodic pain and swelling. On physical examination, a papule with suppuration in the alveolar mucosa in the right side of the mandible was observed. Panoramic radiography and CBCT showed a mixed lesion surrounded by sclerotic bone. The patient was diagnosed with chronic osteomyelitis in association with FOD and treated with antibiotic therapy together with surgical curettage. The incidence, etiophatology, differential diagnosis, treatment and prevention of secondary osteomyelitis associated with FOD are discussed in the light of literature. This information might assist the dentists while choosing the best treatment options for similar cases.

RESUMEN: La displasia ósea florida (DOF) es una forma de displasia ósea donde el hueso trabecular normal es sustituido por tejido conectivo fibroso y tejido mineralizado amorfo. En general, las lesiones son de origen asintomático y los pacientes deben ser seguidos con el examen clínico. Sin embargo, debido a la pobre vascularización de la lesión y al trauma local, pueden producirse lesiones secundarias y osteomielitis. Los pacientes pueden presentarse con dolor, ulceración mucosa, lesión de exposición en la cavidad oral, fístula y edema. En estos casos, el correcto diagnóstico y tratamiento de la lesión es decisivo para reestabilizar la salud y la calidad de la vida. El objetivo de este artículo consistió en presentar un caso de complicación secundaria crónica de osteomielitis tratados con tratamiento conservador. El paciente, negro, de 68 años de edad, consulta por "hinchazón de las encías" que se presentó durante once años, con episodios de dolor. En el examen intraoral, se observó una pápula con supuración en la mucosa alveolar en el lado derecho de la mandíbula. La radiografía panorámica y CBCT mostraron una lesión mixta rodeada de hueso esclerótico. El paciente fue diagnosticado con osteomielitis crónica en asociación con DOF y fue tratado con tratamiento antibiótico junto con curetaje quirúrgico. La incidencia, la etiopatología, el diagnóstico diferencial, el tratamiento y la prevención de la osteomielitis secundaria asociada con DOF se discuten a la luz de la literatura. Esta información puede ayudar a los dentistas a elegir las mejores opciones de tratamiento para casos similares.

Comparative Performance of the Reverse Algorithm Using Architect Syphilis TP Versus the Traditional Algorithm Using Rapid Plasma Reagin in Florida's Public Health Testing Population

In Florida, where syphilis is a reportable disease, the number of primary and secondary (P&S) syphilis cases has increased from 3,266 in 2008–2010 to 5,340 in 2013–2015, a 63% increase. The objective of this study was to compare the performance and sensitivity of the syphilis reverse algorithm with the traditional algorithm for detecting P&S (infectious) syphilis cases. Clinical specimens from individuals who self-referred for syphilis testing at public health clinics were processed using the traditional algorithm (non-treponemal rapid plasma reagin (RPR) test followed by a confirmatory treponemal (EIA) test) and then further tested with the Architect Syphilis TP (ASTP) immunoassay (Abbott Diagnostics, Chicago, IL, USA) or by RPR confirmation, if needed (reverse algorithm). Of 1,079 specimens, 59 were positive for syphilis. The sensitivity of the reverse algorithm was 98.3% (58/59) and of the traditional algorithm was 72.9% (43/59). Based on clinical evidence, of the 16 traditional algorithm-negative but reverse algorithm-positive cases, 68.8% (11/16) were classified as missed P&S infections (treatment naïve) and 31.2% (5/16) were classified as missed past syphilis (latent or infections with documented linkage to care). The reverse algorithm enables the detection of additional P&S syphilis cases missed by our current traditional algorithm.

Importância dos aspectos imaginológicos no plano de tratamento da displasia óssea florida: relato de caso / Importance of imaginological aspects in the florid osseous Dysplasia treatment plan: case report

A displasia óssea florida (DOFL) é uma lesão fibro-óssea relativamente incomum dos maxilares, que acomete, principalmente, mulheres melanodermas de meia idade e geralmente é diagnosticada em radiografias de rotina. O presente trabalho tem como objetivo relatar o caso de uma paciente de 50 anos de idade, melanoderma, encaminhada ao Curso de Odontologia da Universidade Federal do Ceará para avaliação imaginológica. Clinicamente, não foi observada nenhuma alteração de volume de estruturas faciais ou intraorais. A radiografia panorâmica evidenciou imagens radiopacas circunscritas por halos radiolúcidos, localizadas bilateralmente em região edêntula de corpo mandibular, sugestivas de DOFL. Na tomografia computadorizada de feixe cônico (TCFC), observaram se áreas hiperdensas em regiões mandibulares edêntulas, sem expansão de corticais ósseas vestibulares e linguais, e recobertas por fina espessura de tecido ósseo, típicos da displasia. Diante do diagnóstico clínico-imaginológico de DOFL, a paciente foi encaminhada para tratamento odontológico multidisciplinar. Por meio da TCFC, foi possível se estabelecer um plano de tratamento que consistiu em procedimentos minimamente intervencionistas, considerando os aspectos clínicos e imaginológicos das lesões observadas. Em sumário, o presente trabalho reforça a importância do exame clínico meticuloso aliado à avaliação por meio de TCFC, com fins de embasar um plano de tratamento adequado em casos de DOFL assintomáticos... (AU)

Florid osseous dysplasia (FOD) is a fibrous lesion relatively uncommon of maxillary bones, mainly affects middle-age black women and generally is diagnosed on routine radiographs. This paper aims to report a case of black woman, aged 50 years, referred to Dentistry Faculty of Federal University of Ceará for imaging evaluation. Clinically, there were no facial or intraoral structures alterations. Panoramic radiography showed radiopaque images circumscribed by radiolucent halos, located bilaterally in the edentulous mandibular body region suggestive of FOD. Cone beam computed tomography (CBCT) showed hyperdense areas in edentulous mandibular regions, with no expansion of buccal and lingual cortical bone, and covered by fine thickness of bone tissue, characteristic of FOD. After the clinical-imaging diagnosis of FOD, the patient was referred for multidisciplinary dental treatment. Through the CBCT, it was possible to establish a treatment plan that consisted of minimally interventional procedures considering the clinical and imaging limits of the observed lesions. In summary, the present case emphasizes a meticulous clinical examination along with an evaluation by means of CBCT allowing an adequate treatment plan for asymptomatic FOD... (AU)

Florid cemento-osseous dysplasia: a contraindication to orthodontic treatment in compromised areas

ABSTRACT Florid cemento-osseous dysplasia is a sclerosing disease that affects the mandible, especially the alveolar process, and that is, in most cases, bilateral; however, in some cases it affects up to three or even four quadrants. During the disease, normal bone is replaced with a thinly formed, irregularly distributed tissue peppered with radiolucent areas of soft tissue. Newly formed bone does not seem to invade periodontal space, but, in several images, it is confused with the roots, without, however, compromising pulp vitality or tooth position in the dental arch. There is no replacement resorption, not even when the images suggest dentoalveolar ankylosis. Orthodontists should make an accurate diagnosis when planning treatments, as this disease, when fully established, is one of the extremely rare situations in which orthodontic treatment is contraindicated. This contraindication is due to: (a) procedures such as the installment of mini-implants and mini-plaques, surgical maneuvers to apply traction to unerupted teeth and extractions should be avoided to prevent contamination of the affected bone with bacteria from the oral microbiota; and (b) tooth movement in the areas affected is practically impossible because of bone disorganization in the alveolar process, characterized by high bone density and the resulting cotton-wool appearance. Densely mineralized and disorganized bone is unable to remodel or develop in an organized way in the periodontal ligaments and the alveolar process. Organized bone remodeling is a fundamental phenomenon for tooth movement.

RESUMO A displasia cemento-óssea florida é uma doença óssea esclerosante exclusiva dos maxilares, relacionada ao osso do processo alveolar e, na maioria dos casos, envolvendo bilateralmente a mandíbula; mas há casos em que envolve três ou até os quatro quadrantes. Nesse processo, troca-se o osso normal por um tecido densamente formado, irregularmente distribuído e salpicado por áreas radiolúcidas com tecido mole. O osso neoformado parece não invadir o espaço periodontal, mas, em muitas imagens, confunde-se com as raízes, sem comprometer a vitalidade pulpar e a posição dentária na arcada. Não há reabsorção dentária por substituição, mesmo quando as imagens sugerem anquilose alveolodentária. Um diagnóstico preciso por parte do ortodontista deve ser feito em seus planejamentos, visto que essa doença, quando se encontra plenamente instalada, representa uma das raríssimas situações em que o tratamento ortodôntico está contraindicado. Nesses casos, o tratamento ortodôntico está contraindicado porque: a) alguns procedimentos, como a aplicação de mini-implantes e miniplacas, manobras cirúrgicas para tracionamento de dentes não irrompidos e exodontias, devem ser evitados, para se impedir a entrada de bactérias da microbiota bucal no osso comprometido; e b) a possibilidade de movimentação dos dentes nas áreas comprometidas praticamente inexiste, pela desorganização óssea no processo alveolar, caracterizada por elevada densidade óssea, que gera as imagens tipo flocos de algodão. O osso densamente mineralizado e desorganizado não é capaz de se remodelar e desenvolver organizadamente, nos ligamentos periodontais e no osso do processo alveolar. A remodelação óssea organizada é fundamental como um dos fenômenos necessários para o deslocamento dos dentes.

Cytotoxic Activities of Total Saponins from Plena Clematis on Human Tumor Cell Lines In Vitro / 中国结合医学杂志

OBJECTIVE@#To investigate the anti-proliferative effects of saponins prepared from Plena Clematis (PC) cultured in Fujian Province, China on 4 human tumor cell lines and its possible anti-tumor mechanism.@*METHODS@#The growth inhibition assays of saponins on human esophageal squamous carcinoma cell line (EC9706), human hepatoma cell line (HepG-2), human oral cancer cell line (KB) and human gastric cancer cell line (BGC-823) were evaluated in vitro by thiazolyl blue (MTT) method. The inhibitory effects on EC9706 treated with different concentrations of saponins (15.62, 31.25, 62.50, 125, 250 and 500 μg/mL) were performed in vitro by MTT method. The morphology and nuclear staining with acridine orange/ethidium bromide of EC9706 cells treated with saponins were illustrated under an inverted phase fluorescence microscope. The apoptotic effects of saponins were further evaluated by annexin-V/propidium iodide dual staining experiment to examine the occurrence of phosphatidylserine externalization onto the cell surface by a flflow cytometer.@*RESULTS@#MTT assay showed that the saponins could inhibit the proliferation of 4 tumor cell lines. Among them, the maximum inhibition rate of 73.1% was detected in EC9706 cells at the saponins concentration of 250 μg/mL for 24 h. Further investigation indicated that the saponins induced EC9706 cells apoposis. The EC9706 cells presented apoptotic characteristics when treated with saponins, including that the morphologies of EC9706 cells were appeared round-shaped with higher refraction, and the cell nuclear stained orange with EB after 250 μg/mL saponins exposure. The flow cytometry analysis results showed that the induction of cell cycle arrest in apoptotic system may participate in the anti-proliferative activity of saponins on EC9706 cells.@*CONCLUSION@#The saponins from PC exhibited significant cytotoxicity against human EC9706, KB, BGC-823, and HepG-2 cells and might be beneficial to development of ethnic pharmaceutical plant for potential anti-tumor drugs.

Evaluation on clinical application of Florida probes in probing peri-implant soft tissue / 吉林大学学报(医学版)

Objective: To detect the peri-implant soft tissue with Florida probes, and to provide the clinical basis for choosing the appropriate clinical examination method, probing strength, and treatment of peri-implant soft tissue in the maintenance period. Methods: The common periodontal probes and Florida probes were used to examine the probing depth (PD) values in 62 patients who underwent implantation for more than 6 months for two times, and the contralateral teeth were natural teeth. The natural teeth and the implants were divided into inflammation group (n=32) and healthy group (n=30) according to whether the bleeding was probed. The coefficient of variation (CV) values of PD values of natural teeth and peri-implant soft tissues detected by two probes were compared. Results: There were no statistically significant differences in the PD values between natural teeth and implants of the patients measured by common periodontal probes in healthy and inflammation groups (P> 0. 05). There were no statistically significant differences in the PD values between natural teeth and implants of the patients measured by Florida probes in healthy and inflammation groups (P>0. 05). The CV value of PD value detected by Florida probes of the patients in healthy group was less than that detected by common periodontal probes (t=2. 489, P=0. 019); the CV value of PD value detected by Florida probes of the patients in inflammation group was less than that detected by common periodontal probe (t=2. 238, P = 0. 033). The CV value of PD value of implants of the patients in inflammation group was higher than that in healthy group (Z=3. 804, P0. 05). Conclusion: Both two probes have good reproducibility in probing the healthy and inflammatory sites of the natural teeth and implants. The Florida probes are more reproducible than the common periodontal probes in probing the healthy and inflammatory peri-implant soft tissues.

Effect and Mechanism of Total Saponins from Clematis Florida Thunb. in Adjuvant-induced Arthritis Rats / 中国药师

Objective:To investigate the effect of total saponins from Clematis florida Thunb. on rat model of adjuvant arthritis ( AA) . Methods:Wistar rats were randomly divided into six groups:the normal control group, the model control group, the positive control group ( indomethacin, 10 mg?kg-1 ) , high, medium and low dose groups of total saponins from Clematis florida Thunb. with ten rats in each group. The rat model of AA was established with Freund's complete adjuvant and the effects of drugs on body weight and foot swelling of AA rats were observed. After the animals were sacrificed, the serum levels of superoxide dismutase ( SOD) , mal-onaldehyde ( MDA) , nitric oxide ( NO) and glutathion peroxidase ( GSH-PX) were determined. The serum levels of interleukin-8 ( IL-8), IL-10 and tumor necrosis factor-α(TNF-α) were detected by ELISA. Results:On the 8th day of drug administration, total sapo-nins from Clematis florida Thunb. could effectively alleviate the changes in the body weight and foot swelling of the rats to a certain ex-tent when compared with the model control group (P <0. 05 or P <0. 01). There were no significant differences between the high dose group and the positive control group (P>0. 05), and there were significant differences between the low dose group and the high/medium dose groups (P<0. 05). The levels of SOD, MDA, NO and GSH-PX in serum of AA rats were significantly affected by the total saponins from Clematis florida Thunb. . Except for NO indicator of low dose group, the differences between the other groups and the model control group were statistically significant (P<0. 05 or P<0. 01), and there were significant differences in MDA, NO and GSH-PX levels between high dose group and low dose group (P<0. 05). Compared with those in the model control group, the serum levels of IL-8 and TNF-αin each total saponins from Clematis florida Thunb. group significantly decreased and the content of IL-10 sig-nificantly increased (P<0. 05 or P<0. 01). There was no significant differences in the levels of IL-10 and TNF-α between the high dose group and the positive control group (P>0. 05), and the differences in IL-8 and IL-10 between high dose group and low dose group were statistically significant (P<0. 05). Conclusion: The total saponins from Clematis florida Thunb. have good therapeutic effect on AA, and the underlying mechanism may be related to the reduction of lipid peroxidation and the inhibition of pro-inflammatory cytokines.

Effect and Mechanism of Total Saponins from Clematis Florida Thunb. in Adjuvant-induced Arthritis Rats / 中国药师

Objective:To investigate the effect of total saponins from Clematis florida Thunb. on rat model of adjuvant arthritis ( AA) . Methods:Wistar rats were randomly divided into six groups:the normal control group, the model control group, the positive control group ( indomethacin, 10 mg?kg-1 ) , high, medium and low dose groups of total saponins from Clematis florida Thunb. with ten rats in each group. The rat model of AA was established with Freund's complete adjuvant and the effects of drugs on body weight and foot swelling of AA rats were observed. After the animals were sacrificed, the serum levels of superoxide dismutase ( SOD) , mal-onaldehyde ( MDA) , nitric oxide ( NO) and glutathion peroxidase ( GSH-PX) were determined. The serum levels of interleukin-8 ( IL-8), IL-10 and tumor necrosis factor-α(TNF-α) were detected by ELISA. Results:On the 8th day of drug administration, total sapo-nins from Clematis florida Thunb. could effectively alleviate the changes in the body weight and foot swelling of the rats to a certain ex-tent when compared with the model control group (P <0. 05 or P <0. 01). There were no significant differences between the high dose group and the positive control group (P>0. 05), and there were significant differences between the low dose group and the high/medium dose groups (P<0. 05). The levels of SOD, MDA, NO and GSH-PX in serum of AA rats were significantly affected by the total saponins from Clematis florida Thunb. . Except for NO indicator of low dose group, the differences between the other groups and the model control group were statistically significant (P<0. 05 or P<0. 01), and there were significant differences in MDA, NO and GSH-PX levels between high dose group and low dose group (P<0. 05). Compared with those in the model control group, the serum levels of IL-8 and TNF-αin each total saponins from Clematis florida Thunb. group significantly decreased and the content of IL-10 sig-nificantly increased (P<0. 05 or P<0. 01). There was no significant differences in the levels of IL-10 and TNF-α between the high dose group and the positive control group (P>0. 05), and the differences in IL-8 and IL-10 between high dose group and low dose group were statistically significant (P<0. 05). Conclusion: The total saponins from Clematis florida Thunb. have good therapeutic effect on AA, and the underlying mechanism may be related to the reduction of lipid peroxidation and the inhibition of pro-inflammatory cytokines.

Displasia ósea florida. Reporte de un caso y revisión de la literatura / Florid osseous displasia. Case report and literatura review

Las displasias óseas son procesos idiopáticos ubicados en la región periapical del complejo maxilar mandibular caracterizados por un reemplazo de hueso normal por tejido fibroso y hueso metaplásico. La displasia ósea florida (DOF) se refiere a un conjunto de lesiones periapicales e interradiculares radiolúcidas y radiopacas ubicadas en la región mandibular bilateral y ocasionalmente en el maxilar. El presente trabajo tiene como objetivo presentar un caso de DOF diagnosticada mediante hallazgos clínicos, radiográficos e histológicos, con presencia de sintomatología dolorosa e infección. Se presenta femenina de 62 años tratada en la Unidad de Cirugía Buco-Maxilofacial del Hospital General del Este, «Dr. Domingo Luciani¼, Caracas, Venezuela, quien refiere inicio de enfermedad actual dos semanas previas a la consulta presentando dolor intenso en región posterior mandibular derecha con presencia de fístula intraoral y secreción purulenta. Al examen radiográfico se observaron múltiples lesiones radiopacas que involucraban los cuatro cuadrantes. Se realizó biopsia excisional y el estudio histopatológico concluyó displasia ósea florida. La paciente fue evaluada durante un periodo postoperatorio de 11 meses asintomático.

Osseous dysplasias are idiopathic processes located in the periapical region of the maxillary-mandibular complex. They are characterized by the substitution of normal osseous tissue for fibrous tissue and metaplastic osseous tissue. Florid osseous dysplasia (FOD) describes a set of radio-lucid and radio opaque inter-radicular and periapical lesions, bilaterally found in the mandible and sometimes in the upper jaw. The present project presented a FOD case diagnosed by means of histological, radiological and clinical findings; there was presence of infection and painful symptoms. A 62 year old female patient treated at the Oral-Maxillofacial Surgery Unit of the General Hospital «Dr. Domingo Luciani¼, Caracas, Venezuela sought medical care. The patient reported onset of the condition two weeks before consultation, she experienced intense pain in the right mandibular posterior region with presence of intra-oral fistula and purulent discharge. X-ray examination revealed multiple radio-opaque lesions involving all four quadrants. Excision biopsy was undertaken, histopathological study indicated presence of florid osseous dysplasia. The patient was assessed during an 11 month asymptomatic post-operative period.

Determination of Active Ingredient Oleanolic Acid in She Medicine Clematis Florida var. Plena by HPLC / 中国药师

Objective:To establish a method for the determination of oleanolic acid in She medicine Clematis florida var. plena. Methods:The HPLC method was carried on a Lanbo-Kromasil C18 chromatography column using acetonitrile-water ( 86∶ 14 ) as the mobile phase. The detection wavelength was 205 nm, the flow rate was 1. 0 ml·min-1 , and the column temperature was 25 ℃. Re-sults:The calibration curve of oleanolic acid was linear within the range of 0. 52-15. 60μg (r=0. 999 9). The average recovery of oleanolic acid was 92. 13%(RSD=3. 0%, n=9). Conclusion:The method is simple, rapid, accurate and reproducible, which can be used as an ideal method for the quality control of She medicine Clematis florida var. plena. .

Displasia cemento-óssea florida: uma das poucas contraindicações de implantes osseointegráveis / Florid cemento-osseous dysplasia:one of the few contraindications to osseointegrated implants

A Displasia Cemento-Óssea Florida representa uma das poucas situações clínicas de contraindicação para a colocação de implantes osseointegráveis. Como qualquer outro procedimento cirúrgico, inclusive a biópsia, se realizada, a colocação de implante pode abrir as portas para as bactérias acessarem o ambiente ósseo. O osso altamente esclerosado e irregular representa um meio adequado para a proliferação bacteriana e constituição de exuberantes biofilmes microbianos, impedindo o acesso ao local das células e moléculas da defesa orgânica, assim como de antibióticos que, por ventura, venham a ser administrados para o tratamento de uma Osteomielite Crônica Purulenta Secundária, muito comumente vista em pacientes com Displasia Cemento-Óssea Florida. A doença representa um distúrbio dos maxilares no processo de remodelação óssea e, apesar de sua elevada frequência, ainda não se sabe suas causas ou fatores associados, exceto sua predominância em pessoas com alguma afrodescendência, especialmente em mulheres de meia idade...

Florid cemento-osseous dysplasia represents one of the few clinical contraindications to osseointegrated implant placement. As in any other surgical procedure, including biopsy, implant placement might open up the doors to bacteria access to the bone environment. Highly sclerosed irregularbone is appropriate for bacteria proliferation and formation of exuberant microbial biofilm, thereby hindering access not only of local cells and molecules of organic defense, but also of antibiotics potentially administered to treat secondary purulent chronic osteomyelitis commonly found inflorid cemento-osseous dysplasia patients. The disease is a disorder of the maxilla, established during the process of bone remodeling; and despite its high frequency, its causes or associated factors remain unknown, except for its predominance among afrodescendents, especially middle-aged women...

O reconhecimento de expressões faciais e prosódia emocional: investigação preliminar em uma amostra brasileira jovem / Recognition of emotional facial expressions and of emotional prosody: a preliminary investigation in a young brazilian sample / El reconocimiento de las expresiones faciales y la prosodia emocional: investigación preliminar en una muestra brasileña joven

Sinais não verbais de comunicação emocional são frequentes em diferentes culturas. Esta pesquisa buscou traduzir e adaptar uma bateria (Florida Affect Battery) de avaliação de expressões faciais e prosódia emocional, bem como avaliar o desempenho de uma amostra populacional brasileira em seus subtestes, incluindo uma comparação entre participantes do sexo feminino e masculino e por escolaridade. Os resultados da amostra brasileira foram avaliados e, posteriormente, comparados aos da amostra estadunidense, fornecidos no manual da bateria. Não foram encontradas diferenças estatisticamente significantes entre o desempenho da amostra brasileira e da amostra estadunidense, bem como entre homens e mulheres, com altas taxas de acerto para todos os grupos avaliados, evidenciando um bom reconhecimento de expressões faciais e prosódia emocional...

Nonverbal signs of emotional communication are common in different cultures. This study aimed to translate and adapt a battery (Florida Affect Battery) of evaluation of facial expressions and emotional prosody, as well as evaluate the performance of a Brazilian sample in their subtests, including a comparison of men and women and of education level. The results of the Brazilian sample were compared to the results of United States sample provided in battery's manual. There were no significant differences between the performance of Brazilian sample and the United States sample as well as between men and women, with high success rates for all groups evaluated...

Señales no verbales de comunicación emocional son comunes em diferentes culturas. Este estudio tuvo como objetivo traducir y adaptar una batería (Florida Affect Battery) de evaluación de expresiones faciales y prosodia emocional, así como evaluar el desempeño de una muestra de la población brasileña en sus subpruebas, incluyendo uma comparación entre participantes hombres y mujeres e entre los diferentes níveles de educación. Los resultados de la muestra brasileña fueron evaluados, y posteriormente, se compararon con los resultados de la muestra estadounidense, proporcionados en el manual de la batería. No hubo diferencias significativas entre el desempeño de la muestra brasileña y la muestra estadunidense, así como entre hombres y mujeres, con altas tasas de acierto para todos los grupos evaluados, evidenciando un buen reconocimiento de expresiones faciales y prosodia emocional...

Florida Sleeve Repair for Aortic Root Aneurysm

A 74-year-old man was diagnosed with aortic root aneurysm and two-vessel coronary disease. Echocardiographic assessment revealed an enlarged sinus of Valsalva 60 mm in diameter with mild aortic regurgitation. Florida sleeve repair was performed using a vascular graft combined with coronary artery bypass grafting. The postoperative course was uncomplicated and follow-up echocardiographic evaluations showed an aortic root diameter of 38 mm without aortic insufficiency up to 1 year after surgery.

Urgent Work: Developing a Gender-Responsive Approach for Girls in the Juvenile Justice System / Trabajo urgente: desarrollando una respuesta con perspectiva de género para niñas en el Sistema de Justicia Juvenil

This paper reviews the prevalence of girls in the U.S. juvenile justice system, compares national and international incarceration rates, and reviews the profile needs of justice-involved girls. The authors offer their Model as an example of how to develop a gender-responsive approach to girls in the justice system, including a description of how the model was operationalized in a community in the United States. Critical developments and emerging opportunities for each of the Model's components: advocacy, model programming, public education, training and technical assistance, gender responsive tools, systems accountability, and evaluation are highlighted. Lessons learned are offered as a springboard for conversations about how the international community can individually assess their needs and resources and work together to improve the response to girls. The paper concludes with recommendations for choosing, evaluating, and implementing best-practice approaches for meaningful reform.

Este artículo revisa la prevalencia de niñas en el Sistema de Justicia Juvenil de Estados Unidos, compara las tasas de encarcelamiento nacional e internacional y examina las necesidades de un perfil de las niñas involucradas. Los autores ofrecen su modelo como ejemplo para desarrollar una aproximación dirigida al género femenino en el Sistema de Justicia, incluyendo una descripción de su operacionalización en una comunidad de los Estados Unidos. En los desarrollos críticos y la emergencia de oportunidades para cada uno de los componentes del modelo, se destacan: la promoción legislativa y las políticas, el modelo de programación, la educación pública, la capacitación y asistencia técnica, las herramientas sensibles al género, los sistemas de responsabilidad y la evaluación. Las lecciones aprendidas se presentan como plataforma para la interlocución sobre la manera en que la comunidad internacional puede, en forma individual, evaluar necesidades y recursos, y trabajar conjuntamente para dar una mejor respuesta a las niñas. El artículo concluye con recomendaciones para escoger, evaluar e implementar mejores prácticas para una reforma significativa.

Produção de lacase de fungos basidiomicetos por fermentação submersa com cascas de café / Produccin de lacase de hongos basidiomicetos por fermentación sumergida con cáscaras de café / Laccase production from basidiomycetes by submerged fermentation with coffee hukss

Cascas de café são fonte de carboidratos e nutrientes que podem ser bioconvertidos em produtos de interesse como enzimas. Lacases são cobre polifenol oxidases que oxidam compostos fenólicos, enquanto reduzem oxigênio molecular à água e; sua baixa especificidade a substratos permite sua aplicação em várias áreas como indústria têxtil, de alimentos e biorremediação. Os objetivos desse trabalho foram avaliar a capacidade de produção de lacase de três linhagens de fungos basidiomicetos (Lentinula edodes U6/1, Pleurotus ostreatus U6/9 e Pleurotus florida U6/10) por fermentação submersa com cascas de café e avaliar o uso de cobre como indutor dessa enzima. A casca de café mostrou ser um bom substrato para produção de lacases e das três linhagens testadas Pleurotus ostreatus (U6/9) foi a mais produtiva (22,5 U mL-1). A melhor fonte de nitrogênio para produção de lacases de Pleurotus ostreatus (U6/9) foi o extrato de levedura na concentração de 9 g/L (20 U mL-1). A adição de 150 µM de CuSO4 resultou na indução significativa na produção de lacases nessa linhagem (21 U mL-1)no 12° dia de cultivo.

Coffee husks are a source of carbohydrates and nutrients that may be bioconverted into products of interest, such as enzymes. Laccases are copper polyphenol oxidases that oxidize phenolic compounds while reducing molecular oxygen to water. Laccase?s low specificity to substrates allows its application in several areas such as textiles, food processing and bioremediation industries. The aims of this study were to evaluate the potential to produce laccase from three strains of basidiomycetous fungi (Lentinula edodes U6/1, Pleurotus ostreatus U6/9, and Pleurotus florida U6/10) by submerged fermentation with coffee husks, and to evaluate the use of copper as an inducer of the enzyme. Coffee husk proved to be a good substrate for laccase production, with Pleurotus ostreatus (U6/9) being the most productive strain (22.5 U mL-1). The best source of nitrogen for laccase production of Pleurotus ostreatus (U6/9) was yeast extract 9 g/L (20 U mL-1). The addition of CuSO4 (150 µM) resulted in significant induction of laccase (21 U mL-1) on the 12th day of cultivation.

Cáscaras de café son fuente de carbohidratos y nutrientes que pueden ser bioconvertidos en productos de interés, tales como enzimas. Lacases son cobre polifenol oxidasas que oxidan compuestos fenólicos, mientras reducen el oxígeno molecular a el agua y; su baja especificidad a sustratos permite su aplicación en diversas áreas, como la industria textil, de alimentos y de biorremediación. Los objetivos de este estudio fueron evaluar la capacidad de producción de lacase de tres linajes de hongos basidiomicetos (Lentinula edodes U6/1, Pleurotus ostreatus U6/9 y Pleurotus florida U6/10) por fermentación sumergida con cáscaras de café, y evaluar el uso del cobre como inductor de esta enzima. La cáscara de café resultó ser un buen sustrato para la producción de lacases y, de las tres linajes probadas, Pleurotus ostreatus (U6/9) fue la más productiva (22,5 U mL-1). La mejor fuente de nitrógeno para la producción de lacases de Pleurotus ostreatus (U6/9) fue el extracto de levadura a una concentración de 9 g L-1 (20 U mL-1). La adición de 150 µM de CuSO4 resultó en la inducción significativa de la producción de lacases en esa linaje (21 U mL-1), en el 12º día de cultivo.