ABSTRACT
Objective To improve the quality standard of Flos Chrysanthemum Indicum from diffrent origins by analyzing on linarin and cumambrin A qualitatively and quantitatively. Methods Qualitative analysis of linarin and cumambrin A was carried out by thin layer chromatography (TLC); Content determination of linarin and cumambrin A was using high performance liquid chromatography (HPLC) on YMC C18 column (250 mm × 4.6 mm, 5 μm); The mobile phase was a mixture of acetonitrile-0.05% phosphoric acid solution; The elution mode was gradient system (0-18 min, 25%-26% A; 18-26 min, 26%-32% A; 26-33 min, 32%-34% A; 33-35 min, 34%-40% A; 35-65 min, 40%-50% A); The flow rate was 0.8 mL/min; The detection wavelengths were 203 nm and 340 nm; The column temperature was 35 ℃. Results Linarin and cumambrin A by TLC was obvious. The concents of linarin and cumambrin A in Flos Chrysanthemum Indicum from diffrent origins were different. The concents of linarin and cumambrin A from Xinyang were the highest (6.53% and 0.81% respectively). Conclusion It is the first time to establish a method to evaluate different components in Flos Chrysanthemum Indicum by TLC and HPLC. The method is simple, accurate and reproducible, which can effectively improve the existing quality standard of Flos Chrysanthemum Indicum. The result also showed Linarin and cumambrin A could reflect the quality of Flos Chrysanthemum Indicum.
ABSTRACT
OBJECTIVE: To observe therapeutic effects and mechanisms of total flavonoids of Flos Chrysanthemum indicum (TFC) on liver fibrosis in rats. METHOD: The model of rat liver fibrosis was adopted which was induced by CCl4 injection. The effects of FFC were observed on the levels of serum ALT, AST, HA, LN, PC III and C IV in rats with liver fibrosis, and on liver histopathological 'hanges as well as collagen hyperplasia formation in liver tissue. The gene expression of TGF-β1 was detected by RT-PCR. RESULTS: Compared with the model group, TFC treatment significantly reduced ALT, AST, HA, LN, PC III and CIV content in serum, liver listology in the TFC treated rats was also improved. Moreover TFC could decrease the expression of TGF-beta;1. CONCLUSION: TFC Has a significant curative effect on the liver fibrosis rats, its mechanism probably be related to decrease the expression of TGF-beta;1.