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Objective To determine the content of chlorogenic acid, total flavones, and anti-oxidant activity in Flos Lonicerae japonicae and Flos Lonicerae obtained from three different origins and compare their differences. Methods The optimized extraction conditions of chlorogenic acid were ultrasonic extraction 30 min in 65 ℃ with ethanol: water (60︰40) and solid-liquid ratio (1︰20). The optimized extraction conditions of total flavonoids were ultrasonic extraction 30 min at 65 ℃ with 60% methanol solution, solid-liquid ratio (1︰10). HPLC and UV spectrophotometry were used to determine the content of chlorogenic acid and total flavonoids in the samples, and then estimation of anti-oxidative activity of Flos Lonicerae japonicae and Flos Lonicerae by DPPH radical scavenging capacity method. Results A method for the analysis of chlorogenic acid and total flavonoids was established, which have a good linear relationship of chlorogenic acid in 0.119-1.190 mg/mL and R2 was 0.999 2 (n = 6); A good linear relationship between 0.008 and 0.050 mg/mL and r2 was 0.999 5 (n = 6) for analysis of the total flavonoids. The average content of chlorogenic acid, total flavonoids, and free radical scavenging rate of Flos Lonicerae from Hunan Province was 3.99%, 13.43%, and 62.41%, respectively. The average content of chlorogenic acid, total flavonoids and free radical scavenging rate of Flos Lonicerae from Chongqing were 3.29%, 10.08%, and 51.48% respectively. The average content of chlorogenic acid, total flavonoids, and free radical scavenging rate of Flos Lonicerae japonicae from Guangxi Province were 2.55%, 7.10%, and 39.51%, respectively. Conclusion This study proposed an analytical method combining the chemical composition analysis and anti-oxidant activity to compare the differences between the different producing areas of Flos Lonicerae japonicae and Flos Lonicerae. Combining the “spectrum-effect”, it provides a new model for the quality control and identification of two plants.
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Objective To optimize and establish the extraction of total flavones in Lonicera japonica. Methods The ultraviolet spectrophotometry was used to determinate the total flavonoids. The effect of extraction time, the amount of solvent and extraction times,pH were tested by the orthogonal design.ResultsThe total flavonoids showed a linear relationship,the optimal extraction condition of total flavones in Lonicera japonica was with 16 times volume of sample weight water for three times, each time was 2 h.Conclusions The method is simple and applicable to the extraction of total flavones in Lonicera japonica.
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Objective To study the technology of flocculation of water-extraction solution in Lonicerae Japonicae Flos by uniform design method.Methods The liquid concentration ratio, chitosan dosage, temperature and pH were studied with the ratio of the precipitation and the rate of the transformation of valid target as index.Results The optimal flocculation process was: dosage of chitosan was 0.14%, pH was 6, the concentration of the solution was 1:3 and the temperature was 30℃.Conclusions The effect of purification is good, and the flocculation process can replace the traditional precipitation process.
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Objective To compare the quality of Lonicerae flos and Lonicerae japonicae flos by determine the main active ingredients. Methods According to methods and standards of determination in Chinese Pharmacopoeia (2015 Edition), the content of chlorogenic acid , galuteolin , macranthoidin B and asperosaponin B in 50 batches of samples of Lonicerae japonicae flos and .Lonicerae flos were determined. Results For Lonicerae japonicae flos (Lonicera japonica Thunb.), the contents of galuteolin was higher, and chlorogenic acid was lower, less or no contain saponins. For the main species of Lonicerae flos (Lonicera macranthoides Hand.-Mazz. and Lonicera hypoglauca Miq.), the contents of chlorogenic acid and the sum of saponins were higher, less or no galuteolin. Conclusions The main active ingredients of Lonicerae japonicae flos and Lonicerae flos were different. The contents of saponins in some samples of Lonicerae japonicae flos were higher, so the test of saponin should be considered when its raw material for injection.
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OBJECTIVE:To establish methods for the determination of the metal elements and the Total flavonoids in Flos Lonicerae Japonicae.METHODS:The atomic absorption spectrometry was adopted to determine the contents of macroelements(Na,K,Ca,Mg)and trace elements(Cu,Zn,Fe,Mn,Cr,Ni)while UV spectrometry was adopted for the determination of the content of Total flavonoids in Flos Lonicerae Japonicae.RESULTS:Metal elements and Flavonoids were found to be rich in Flos Lonicerae Japonicae.CONCLUSION:The methods adopted in this study were simple,rapid,accurate,sensitive and precise.
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AIM: The extent similarity algorithm was introduced, integrated clustering analysis to evaluate the quality of different sources of Flos lonicerae japonicae. METHODS: The fingerprint spectum of Flos lonicerae japonicae was established to calculate correlation coefficient, cosine of the included angle and extent similarity of thirty-five samples, and then clustering analysis was adopted. RESULTS: In combination of the extent similarity and the clustering analysis to evaluate the quality of Flos lonicerae japonicae, and the results showed that it accorded with reality. CONCLUSION: This method is accurate and reliable, and it is an apparent, credible and efficient method for quality evaluation of Chinese medicines.
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AIM: To construct suitable quality standard of evaluating antipyretic effect of 4 herbs,Bupleurum,Flos Lonicerae Japonicae,Radix Isatidis and Fructus Forsythiae. METHODS: Extracts,essential oils and its mixtures of extract and oil of above herbs,plus Ibuprofen Suspension,were used for antipyretic experiment on rats.Principal Component Analysis and Canonical Correlation Analysis were adopted as evaluation tools to examine the correlation between pharmacological effect and UV spectra of herb's chemicals,from which spectral marker of potential reliability for evaluating antipyretic effects of samples could be constructed. RESULTS: Antipyretic effect sequencing of UV spectral canonical variables was consistent with that of the four herbs. CONCLUSION: The first canonical variable U1 expressed by principal component scores of UV spectra of herbs could be used as spectral marker for evaluating antipyretic effects of the four herbs.