ABSTRACT
Objective To investigate the role of bladder interstitial cells of Cajal(ICCs) in regulating the excitability of detrusor myocytes.Methods 1)Ultrastructural connection between ICCs and detrusor myocytes was detected by transmission electron microscope(TEM).2) Fluorescence redistribution after photobleaching(FRAP) was carried out to detect the intercellular communication between detrusor myocytes and its neighboring ICCs.Results 1)Gap junction between the 2 kinds of cells was confirmed by TEM.2)After target detrusor myocyte was photobleached,fluorescence intensity in detrusor myocytes was recovered gradually.In the meantime,fluorescence intensity in the ICCs neighboring to the target detrusor myocyte was decreased.This indicates a signal transmission from the neighboring ICCs to the target detrusor myocyte.Conclusion Structural connection is seen between ICCs and detrusor myocytes.Excitability signals might be transferred from ICCs to detrusor myocyte.
ABSTRACT
Objective To study the functional changes of gap junctional mediated intercellular communication in detrusor instability so as to demonstrate the feasibility of blocking excitatory communication as the target of therapy for DI. Methods The function of GJIC in the cultured bladder detrusor cells were detected by FRAP. Results At the fourth minutes after bleaching,the mean fluorescences recovery rates of the DI groups bladder detrusor cells was (35.791?0.836)%,the control groups (8.645?0.673)%.The mean fluorescences recovery rates of the DI groups were significant higher than those in control groups (P