ABSTRACT
Objective To investigate the effect of focal adhesion kinases (FAK) inhibitor with different concentration on the adhesion and migration of endothelial cells (ECs) and the expression of downstream Rac1 protein, and to explore the role of FAK in adhesion and migration of ECs by using FAK inhibitor to inhibit the phosphorylation of Y397 site of FAK. Method Scratch wound migration assay was performed to examine the effect of FAK inhibitor with different concentration (from 0 nmol/mL to 250 nmol/mL) on ECs migration at 2, 4, 8 and 24 h, respectively. Western blot combined with immunofluorescence analysis were performed to determine the effect of FAK inhibitor with different concentration on distribution and expression of Rac1 protein. Results With the concentration of FAK inhibitor increased, ECs migration distance and the Rac1 protein expression decreased. Conclusions The inhibition of FAK phosphorylation could inhibit cell adhesion and migration with the decrease in downstream Rac1 protein, and ECs adhesion/migration was related to FAK Rho GTPases signaling pathways.
ABSTRACT
Objective: To investigate the correlation between neck lymph node metastasis and focal adhesion kinases(FAK) expression in oral squamous cell carcinoma(OSCC). Methods: Immunohistochemistry LsAB technique was used to observe the expression of FAK in primary tumour and nodal metastasis of OSCC in 80 cases, correlation between the expression of FAK in OSCC and neck lymph node metastasis was analyzed statistically. Results: The results demonstrated that FAK was expressed in all 80 cases, the expression of FAK in the peripheral area of OSCC was stronger than that in central area (P