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Objective To establish a rapid method for the detection of SMCY antigen. Methods To Use the technology of colloidal gold immunochromatography with double antibody sandwich assay, the gold labeled pad was coated with colloidal gold labeled SMCY rabbit polyclonal antibody, colloidal gold strip was made for detection of serum, which include 12 serum samples of pig, cattle, dog, chicken and mice and 50 serum samples of human. Results The colloidal gold test strips showed obvious specificity in human and common animal sera and could distinguish between male and female sera. Conclusion This method can be used to identify the serum of women and men, and has certain species specificity, which provides a direction for forensic science to rapidly identify gender of human samples.
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Objective To study the correlation between the polymorphisms of NR3C1 gene and aggressive behavior in Yunnan Han population.Methods Five SNPs of the NR3C1 gene (rs6190,rs6191,rs6198,rs41423247 and rs56149945) were genotyped in 194 unrelated prisoners who committed violent-crimes and 301 healthy controls using improved Multiplex-ligase-detection reaction(iMLDR) method,and the data were statistically analyzed with the SPSS19.0soflware and PHASE2.1platform.Results Single locus analysis showed that the allelic distribution of rs6191and rs41423247did not show significant differencesbetween the control groupand the aggressive-behavior group as well as the robbery sub-group and intentional injury sub-group.However,significant difference was foundin the rs41423247 genotype distribution betweencontrol groupand robbery sub-group (p=0.048).In addition,there were no significant differences for the four haplotypes between the control group,the attack group,the robbery subgroup and the intentional injury subgroup.Conclusion These findings indicate that rs41423247 polymorphism of the NR3C1gene might play a role in susceptibility to aggressive behavior and rs6191 polymorphismmay not be correlated withaggressive behavior.
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Objective To evaluate the STR profiling availability of formalin-fixed tissues and the detectable time limit of intact STR profile.Methods The different human tissues were fixed with 10-fold diluted commercial 40%formalin fixative for different duration under 15～20℃,and then DNA was extracted using the method of QIAamp~(R)DNA and IQ~(TM) DNA System.The extracted DNA was quantified with QuantifilerTM kit and amplified by both AmpFSTR identifiler kit and AmpFSTR MiniFiler kit.The STR profile was analyzed by GeneMapper ID v3.2 on 3100-Avant.Resuls The STR profiling availability of formalin-fixed tissues was relevant to the formalin fixing duration mainly.as well as the type of tissues and the template concentration and protocol of DNA extracting.The optimal ranges of template concentration is 1～3ng/μL and the QIAamp extracting method was preferable.There are differences in the degradation rate between various types of tissues in the unbuffered formalin fixative,and the lung tissue showed the slowest rate and liver and intestine tissues the fastest.Intact miniSTR profile of all the tissues detected could be obtained within 15 days duration of formalin fixing while intact STR profile could be obtained within 4 days.Conclusion The major factor that impact the availability of STR profiling of formalin-fixed tissues is the fixing duration in unbuffered formalin,as well as the type of tissues,method of extraction,concentration of PCR template and the kinds of STR loci.
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Objective To analyze and evaluate the 48 selected highly polymorphic SNP loci in Chinese Han population on genetic and forensic aspects.Methods Samples from 200 unrelated Han individuals in East China were collected.48 X-SNP polymorphic genetic markers were selected according to the information providedby NCBI and HapMap,and then were typed by SNPlex~(TM) System for establishing genetic data.Results The 48 X-SNP loci were highly polymorphic markers in East China Han population except rs6527549,and their polymorphism information contents(PIC)were all above 0.32,the discrimination power(DP)in females and males were above 0.56 and 0.40 respectively,the probability of exclusion(PE)in duos and trios were above 0.20 and 0.32 respectively.In addition,linkage disequilibriums were observed among some loci.Conclusion The 48 X-SNP loci union is suitable for developing high-throughout automated research and also useful for special parentage testing.
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To introduce tutorial system for undergraduate in the forensic biology teaching is an important measure of educational reform of forensic medicine.The article presented some schemes and experiences about the practice of tutorial system for undergraduate in the forensic biology department of Kunming Medical College.
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Based on the experience of experimental teaching reform of forensic biology,the article made a systematic exposition about the measures and experience of the research and practice of the reform from the following aspects: aim of the reform,priority of the reform and specific measures.By reforming experimental teaching methods,the students' interests and performance record have been significantly improved.
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The article elaborated the standard of quality control,open service management system and requirements for system construction of forensic biology laboratory,which is to share laboratory recourses and advance the healthy and sustainable development of the laboratory.
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Forensic biology is a main subject of forensic medicine.With the rapid development of science and technology,the courses should be constantly improved.New teaching mode has come into being application of multimedia technology in theoretical lessons,case teaching,independent completion of experimental lessons,which is thanks to the constant reinforcement of teaching staff and the reform of course content and teaching methods.
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The reform for the laboratory operational mechanism includes laboratory management system reform and experimental teaching reform.The author made some explorations and research to the operational mechanism and management pattern of university laboratory of forensic biology,then presented some ideas.