ABSTRACT
Aim To explore whether Huaqizeren, an experienced prescription for the treatment of IR, could improve IR by interfering with KDSR expressionfrom the perspective of KDSR gene. Methods The KDSR gene was overexpressed in normal HepG2 cells, and the IR cell model was established at the same time. The expression level of KDSR was detected by QRT PCR and Western blot; the content of ceramide was detected by HPLC-MS; the expression level of KDSR was detected by Western blot. The phosphorylation level of PKC£/Akt/Fox01 was detected by Western blot, and the glucose content was detected by GOD-POD assay before and after administration. Results After administration, the expression level of KDSR and ceramide in IR cells and KDSR overexpression cells significantly decreased, and the abnormal expression of PKC£/Akt/ FoxOl signaling pathway was improved. Meanwhile, the glucose content in IR cells and KDSR overexpres-sion cells significantly decreased after administration, and the differences were statistically significant (P < 0. 01). Conclusions One of the mechanisms of Huaqizeren in improving IR may be down-regulating the expression of KDSR, decreasing the content of ceramide , and then regulating the expression of key proteins in PKC£/Akt/Fox01 signaling pathway, thus reducing the glucose content in cells and improving IR.
ABSTRACT
SD rats were injected with streptozotocin and fed with high fat diet,used as type 2 diabetic model. Transcription factor Fox01 [in nucleus (15.00±1. 15 vs 6.45±0. 62) %, P<0.05], Caspase-3 [(23.73 ±1.48 vs 6.30±2.20)% ,P<0.01] expressions in pancreatic istet β cells and the positive rate of islet β cells apoptosis[(22.29±1.84 vs 6.25±2.42) %, P<0.01] in diabetic rats were higher than those of control rats. The islet cells which highly expresed Fox01 (in nucleus)and caspase-3 were just the apoptotic islet cells. Therefore,Fox01 may be involved in regulating apoptosis of islet β cells in type 2 diabetes.
ABSTRACT
Objective To investigate the changes of the forkhead box transcription factor-1(FOX01) mRNA level in peripheral blood mononuclear cells(PBMC) from type 2 diabetes mellitus(T2DM) patients and to explore the role of FOX01 in pathogenesis of T2DM.Methods PBMC was isolated from 62 T2DM patients and 40 healthy persons.FOX01 mRNA level in PBMC was measured with reverse transcription PCR and real-time fluorescent quantitative PCR.Results FOX01 mRNA level in PBMC from T2DM patients was significantly higher than that from healthy persons(P