ABSTRACT
【Objective】 To analyze the changes of fibrinogen (Fg) and Ⅷ factor levels of cryoprecipitated coagulation factors prepared by different methods and post-preparation quick-freezing time. 【Methods】 The fresh frozen plasma (FFP), prepared from 400mL whole blood, was randomly divided into 6 groups(group A1, A2, A3, B1, B2, B3) with 20 eliquots each, to prepare cryoprecipitate coagulation factors. Group A1, B1 were prepared by automatic cryoprecipitation preparation instrument. group A2, B2 applied the instrument after centrifugation and group A3, B3 were prepared manually. The quick-freezing time after preparation in group A1-A3 and B1-B3 were different(within 1 hour vs. more than 1 hour after preparation). The automated coagulation analyzer was used to measure Fg and Ⅷ factor levels in six groups, and further statistical analysis was carried out. 【Results】 The Fg content (mg) of six groups were 245.29±27.44 in group A1, 227.13±18.68 in group A2, 221.11±20.95 in group A3, 182.12±9.15 in group B1, 163.68±15.50 in group B2, and 155.61±19.28 in group B3, respectively. The Ⅷ factor levels(IU) were of six groups were 115.86±27.99 in group A1, 93.79±36.29 in group A2, 91.92±34.75 in group A3, 83.04±18.82 in group B1, 66.33±19.57 in group B2, and 69.34±13.26 in group B3, respectively. There were no significant differences in gender or age between group As and groups Bs. The levels of Fg and Ⅷ factors in group A1 were significantly higher than those in group A2 and group A3 (P<0.05). In addition, the levels of Fg and Ⅷ factors in group B1 were also obviously higher than those in group B2 and group B3 (P<0.05). Further, the levels of Fg and Ⅷ factors in group As were significantly higher than those in group Bs (P<0.05). 【Conclusion】 The automatic cryoprecipitation preparation instrument plus quick-freezing within 1 hour after preparation contribute to a higher efficiency and better quality than others.
ABSTRACT
BACKGROUND: Cryosurgery uses freezing temperatures to achieve specific effects on tissues, and this method has been used to treat various skin diseases for the last five decades. The modern cryosurgery is especially effective on the treatment of benign, premalignant and malignant lesions including keloid, hypertrophic scar and pigmented disease such as Ota nevus. The results of cryosurgery depend on such factors as types of cryogen, freezing and thawing time, and numbers of the freezing-thawing cycle. OBJECTIVE: The purpose of this study is to show differences among groups of disease, sites of treatment and applying methods through comparing freezing and thawing time. And specifying the optimum applying methods for the treatment of each specific disease is studied. METHOD: To attain the goal of the study, operation records of 224 patients, who received cryosurgical treatment at the Ewha Woman's University Dongdaemoon Hospital, have been analyzed. RESULTS: 1. The numbers of male and female patients of selected group were 74 and 150, respectively, and their ages between 2 and 54 years. Among the group, 42 patients had epidermal pigmentary disease, 77 had dermal pigmentary disease and 105 had scars. 2. Ratios of TT(thawing time) to FT(freezing time) were significantly different among groups of disease(p<0.05). 3. Ratios of TT to FT were significantly different among groups of treatment site(p<0.05). 4. Ratios of TT to FT showed great difference between the group of rolling method applied and one of pressure method applied(p<0.05). CONCLUSION: The output of this study indicates that the result of the cryosurgery tends to be heavily influenced by the surgical method that is employed. It also proves that the pressure method tends to inflict more severe cryoinjury on the tissues than the rolling method. Each different tissue shows different sensitivity to the cold injury, and the longer the thawing time is, the more cryoinjury of tissues occurs. Therefore, to achieve the maximum therapeutic result while minimizing the undesired effect, adequate surgical method should be selected and applied to each specific disease. This concept is introduced in this study as "Theory of Selective Cryolysis.".