ABSTRACT
Objective: To establish the quality standard for Jiuwei Zhaxun pills. Methods: Fructus Chebulae, Flos Carthami and Herba Dracocephali Heterophylli were identified by TLC. HPLC was used to determine the content of gallic acid in Jiuwei Zhaxun pills. Results: TLC showed clear sports without any interference from the negative control. Gallic acid showed a good linear relationship (r=0. 999 7) within the range of 0. 028 6-0. 572 0 μg. The average recovery was 97. 74% with the RSD of 0. 95% (n=6). Conclusion:The methods are with strong specificity, high sensitivity and good reproducibility, which can be applied in the quality control of Jiuwei Zhaxun pills.
ABSTRACT
TLC method for the identification of triterpenoids polyphenols in Fructus Chebulae was established .The results showed that this method had a plentiful chromatogram and good specificity,and can distinguish Fructus Chebulae immaturus from Fructus Chebulae.Determination of gallic acid, one of the hydrolysates of Fructus Chebulae, by HPLC was also reported.The linear range of gallic acid is from 0.17?g to 1.36?g(r=0.9998),and the average recovery is 102.9 %. The results showed that the method is simple, accurate and with good reproducibility.It can be used to determine gallic acid in Chinese herbal medicine that contains hydrolysable tannins.
ABSTRACT
AIM:To study the bioactive components effect of every kind of herbal in Naru Sanwei Pill (Fructus litseae, Fructus chebulae) on Radix Aconiti kusnezoffii. METHODS: Analysising the Naru Sanwei Pill, adding the extraction of Radix Aconiti kusnezoffii to Fructus chebulae and Fructus litseae and was analyzed by ESI-MS~n. RESULTS: The quantity of mesaconitin was low in the extraction of Aconitum kusnezoffii Reichb,and Fructus chebulae.Long Pepper did not have much effect on alkaloids of Aconiti kusnezoffii Reichb. CONCLUSION: Fructus chebulae reduced toxicity in the process of Radix aconiti kusnezoffii,through lowering the dissolving-out of aconitine.