ABSTRACT
Objective To establish a submandibular gland(SMG) cell model of radiation injury, and to observe the effect of compatibility of Fructus Mume(Wumei) and Radix Glycyrrhizae(Gancao) on the proliferation of the modeled cells. Methods SMG cells were primarily cultured in vitro for the experiment. After immunofluorescence identification and growth curve analysis, SMG cells were given one-time exposure to X electron-ray at dose of 0.1, 0.2, 1, 2, 4 Gy respectively, and then were cultured with Wumei-Gancao solution at the concentration of 500, 50, 5, 0.5 μg/mL separately. Methyl thiazolyl tetrazolium (MTT) was used to determine the survival rate of SMG cells exposed to different doses of X electron-ray and proliferation vitality of SMG cells treated with different concentrations of Wumei-Gancao solution for 24 h. Results The survival rate of SMG cells was decreased gradually with the increase of X electron-ray dose. When the concentrations of Wumei-Gancao solution were in the range of 50 ～ 0.5 μg/mL, the cell proliferation rate showed an increasing trend with the increase of drug concentration, the effect being dose-dependent. When the solution concentration arrived to 500 μg/mL, the cell proliferation rate was declined. Conclusion The SMG cell model of radiation injury has been established successfully under the conditions of 0.1 Gy of one-time exposure, 9 Mev of energy at dose rate 3 Gy/min, hop count at 12 MU and source-skin distance being 100 cm. The concentration of the solution with the compatibitity ratio of Wumei to Gancao being 3:1 has certain effects on repairing the modeled cells.
ABSTRACT
Objective:To investigate the effect of decoction of Fructus Mume (DFM) on the myoelectric activity of uterine smooth muscle of unpregnancy and early pregnancy rats and study its action mechanism.Methods: A pair of bipolar electrode was implanted on the serous surface of rat uterus. The DFM was injected into abdominal and the changes of uterine myoelectrical activity were observed.Results: The higher dose of DFM could enlarge the average amplitude of slow wave of uterine smooth muscle myoelectricity as well as increase the incidence and the maximal amplitude of the outbreak plexiform myoelectric wave. On the other hand, rats during early pregnancy were more sensitive to the DFM. Conclusions: The DFM enhances the myoelectricity activity of uterine smooth muscle of unpregnancy and early pregnancy rats which may result from enhacing the start cells electric activity and speeding up the action potential depolarization. Thus the DFM can serve as an effective clinical drug for anti early pregnancy.
ABSTRACT
Objective: To determine oleanolic acid in Fructus Mume. Methods: HPLC with Shim-pack CLC-ODS column was used. The mobile phase was composed of MeOH-H 2O-HOAc-TEA (85∶15∶0.04∶0.02). The flow rate was 0.8mL?min -1 and the detection wavelength was at 204nm. Results: It showed that a good linear relationship existed in the range of 0.8864～4.432?g of oleanolic acid. Its average recovery was 97.03%. RSD was 0.25% (n=5), respectively. Conclusion: This method is quick, accurate and can be used for the determination of oleanolic acid of Fructus Mume.