ABSTRACT
Objective: To investigate the chemical constituents of the fruits of Vitex trifolia Linn. var. simplicifo- lia Cham(Viticis Fructus,VF). Methods: Various column chromatographic techniques(Silica gel,Sephadex LH-20, ODS,and MCI)and preparative high performance liquid chromatography(PHPLC)were employed to separate the chem- ical constituents of the ethanolic extracts of VF. The chemical structures of the isolated compounds were identified accord- ing to the physicochemical properties as well as the NMR and MS data. Results: The isolated fifteen compounds from the ethanol extract of VF were identified as agestricin D(1),5,3’-dihydroxy-6,7,4’-trimethoxyflavanone(2),chryso- splenol D(3),casticin(4),vitetrifolin F(5),4β,10α-aromadendranediol(6),(E)-3,3’-dimethoxy-4,4’-dihydroxystilbene(7),(8R)-evofolin B(8),ω-hydroxypropioguaiacone(9),4(- 4-hydroxyphenyl)-2-butanone(10),4-hy- droxyacetophenone(11),α-hydroxyacetovanillone(12),benzyl-β-D-glucopyranoside(13),picein(14),and 4-(4’- hydroxyphenyl)-2-butanone-4’-O-β-D-glucopyranoside(15),respectively. Conclusion: Compounds 7,8,10,and 13-15 are obtained from the genus Vitex for the first time.