Determination of Dehydroandrographolide in Fufang Kumu Xiaoyan Pian by HPLC / 中国中医药信息杂志

Objective To establish a HPLC method for the assay of Dehydroandrographolide in Fufang Kumu Xiaoyan Pian. Methods Standard was Dehydroandrographolide, HPLC was used with Agilent ZORBAX SB-C18 column (4.6 mm?150 mm, 5 ?m). The mobile phrase was consisted of acetonitrile-0.1% Ammonia slution (30∶70). The detection wavelength at 253 nm. The flow rate was 1 mL/min. Results The assay displayed good linearity. The linear range of ephedrine Hydrochloride was 0.049 92~4.992 ?g (r =0.999 9, n =7). The average recovery was 99.70% and RSD was 0.63% (n =9). Conclusion This method is simple, accurate and can be used for the quality control of Fufang Kumu Xiaoyan Pian.