ABSTRACT
Introduction: Before discovering phospho-diesterase5-blocker erectile dysfunction was considered to treat by psychologists only. Early diagnosing and treating the erectile dysfunction is preventing myocardial infraction and brain stroke. There is not enough evidence based study related to erectile dysfunction with prostate symptoms, aging and testosterone deficiency syndrome.Purpose of our study is investigating the erectile dysfunction men over 40 years comparing to control group.And we studied the relation of erectile dysfunction to prostate symptoms, aging male symptoms and testosterone deficiency syndrome. To determine to late onset hypogonadism by serum level of total testosterone less sensitive than free testosterone. Result:1. Mean IIEF score was in control group 22.38±3.06, respectively in case group 11.36±3.47 (p<0.001).2.There was not statistically significant association with risk factors alcohol, smoking with erectile dysfunction. But IPSS was in control group 10.53±7.22 and in case group 5.79±5.86 (р<0.001).3.Aging male symptoms scale and erectile dysfunction correlated(p<0.002). We detected24.27% (75) of men in this studysuffering from erectile dysfunction with late onset hypogonadismvia using free testosterone.
ABSTRACT
ObjectiveTo construct ompW- and ompW+ mutants of Salmonella paratyphi A with λRed system,and then study the function of the gene preliminarily.Methods Homologous regions were amplified from the genome Salmonella paratyphi A 50973,and then connect with kana fragment from plasmid pET22b-kan to construct a recombinant vector.The resultant fragments were amplified and transferred into 50973 with the help of λRed system after its concentration.Then the ompW- mutant was obtained PCR identification.Connect the recombinase expression plasmid pACU184 with full fragment of ompW regulatory region and coding region,then transfer the connection product into the mutant,the ompW+ mutant was obtained after double digest identification.Full cells of the wild,ompW- and ompW+ mutants were samples for SDS-PAGE and Western blot to detect the expression of protein OmpW.Biochemical identification of wild strain and mutant strains was conducted,so did the growth curves of the wild and the ompW- mutant.Choose BALB/c mice as a model to determine median lethal dose LD50 of the wild and mutant strains in order to observe the correlation between ompW gene and bacterial virulence.ResultsompW gene was knocked out in Salmonella paratyphi A 50973,also the ompW+ mutant was constructed; The wild and ompW+ mutant express the protein OmpW,while the ompW- mutant lost the protein.Each of the wild and mutant strains was Salmonella paratyphi A,and no obvious difference could be observed for their growth curves.LD50 for each strain was also similar.Conclusion The ompW gene has no correlation with the virulence in S.paratyphi A 50973,but the contribution of the mutants made an important foundation for the further study of functions of the gene ompW in Salmonella paratyphi.