ABSTRACT
Non-small-cell lung carcinoma (NSCLC) is one of the most frequently diagnosed malignancies worldwide.Previous studies have shown that microRNA-449b (miR-449b) functions as a tumor suppressor in many cancers.However,the role of miR-449b in NSCLC is still unknown.In the present study,miR-449b was significantly downregulated in NSCLC samples and cell lines.Bioinformatics analysis revealed that 3'-UTR region of leucine rich repeat containing G protein-coupled receptor 4 (LGR4) mRNA had putative complementary sequences to miR-449b,which was further confirmed by the luciferase assay.Western blotting showed that restoration of miR-449b in NSCLC cells decreased the expression of LGR4.Interestingly,over-expression of miR-449b inhibited growth and invasion of NSCLC cells in vitro.Furthermore,ectopic expression of LGR4 reversed miR-449b-suppressed proliferation and invasion of NSCLC cells.Therefore,the data of the present study demonstrate that miR-449b inhibits tumor cell growth and invasion by targeting LGR4 in NSCLC.
ABSTRACT
Non-small-cell lung carcinoma (NSCLC) is one of the most frequently diagnosed malignancies worldwide.Previous studies have shown that microRNA-449b (miR-449b) functions as a tumor suppressor in many cancers.However,the role of miR-449b in NSCLC is still unknown.In the present study,miR-449b was significantly downregulated in NSCLC samples and cell lines.Bioinformatics analysis revealed that 3'-UTR region of leucine rich repeat containing G protein-coupled receptor 4 (LGR4) mRNA had putative complementary sequences to miR-449b,which was further confirmed by the luciferase assay.Western blotting showed that restoration of miR-449b in NSCLC cells decreased the expression of LGR4.Interestingly,over-expression of miR-449b inhibited growth and invasion of NSCLC cells in vitro.Furthermore,ectopic expression of LGR4 reversed miR-449b-suppressed proliferation and invasion of NSCLC cells.Therefore,the data of the present study demonstrate that miR-449b inhibits tumor cell growth and invasion by targeting LGR4 in NSCLC.
ABSTRACT
Objective To explore the effect and diagnostic value of G protein coupled receptor 4 (LGR4) on the establishment of neovascularization of HPV infected cervical cancer tissue.Methods From March 2016 to December 2017,thirty cases of HPV infected cervical cancer,cervical precancerous lesion and cervical health in Lan County Maternal and Child Health and Family Planning Service Center were selected as subjects.After the consent of the stubjects,specimens were collected.Immunohistochemical method was used to observe the positive rate of LGR4 in three kinds of tissue specimens.The diagnostic value of LGR4 in differentiation,staging and typing of cervical cancer was observed by screening test,and the expression of related pro-angiogenic factors in LGR4-positive tissues was observed.Results The difference in the expression of LGR4 in healthy tissue,precancerous lesions and cervical cancer tissues was statistically significant (x2=24.104,P =0.000).The positive rate of LGR4 expression in cervical cancer tissue was 80％ (24/30),which was significantly higher than that in healthy group 16.7％ (5/30) and precancerous lesion tissue 50％ (15/30),the differences were statistically significant (P ＜ 0.05).The sensitivity of LGR4 was 80.00％,the specificity was 83.33％,the positive predictive value was 82.76％,the negative predictive value was 80.65％,the diagnostic accuracy was 81.67％ and the Kappa value was 0.63,which has good consistency.The differences of LGR4 positive rate in different age,different degree of differentiation and different clinical stages were statistically significant (P＜0.05).Laboratory results showed that CRP,PCT,VEGF and TGF-β3 in LGR4-positive tissues of cervical precancerous lesions and cervical cancer were significantly higher than those in LGR4-negative tissues,the differences were statistically significant (t=9.247,2.925,39.669,15.348,P＜0.05).Conclusion LGR4 can promote the establishment of neovascularization in cervical cancer and has certain diagnostic value for cervical cancer and cervical precancerous lesions.