Effects of G. Pentaphyllumon on photoaging skin in nake mice / 中华医学美学美容杂志

Objective To investigate G. Pentaphyllums influence on the activity of glutathione peroxidase (GSH-PX) and catalase (CAT) in the skin of hireless photoaging mice, as well as the content of hydroxyproline (HYP). At the same time, G. Pentaphyllum's effect on the hireless photoaging mice was studied. Methods Healthy KM hireless mice were randomly divided into five groups. A skin photoaging model was established by G. Pentaphyllum administration. The changes of the activity of GSH-PX, CAT and the contents of dermal hydroxyproline in the skin were then measured by biochemical methods in each group of the experiment. Results In the model group, the activity of GSHPX, CAT and the content of HYP of skin were all decreased significantly (P＜0. 05). And there were different degrees of improvements in those of the herb given groups. Conclusion G. Pentaphyllumhas the protective effect on the skin of the photoageing in the hairless mice. The mechanism may be that G. Pentaphyllum could inhibit the oxidative damages of ultraviolet and skin photoaging by increasing the synthesis of collagen in skin.

Quality control of G. pentaphyllum by wavelet transform and fractal expression / 中成药

AIM:To develop the HPLC fingerprinting of Gynostemma pentaphyllum and evaluate the quality of different sources of G.pentaphyllum by fractal expression and wavelet analysis based on similarity calculation.METHODS:Flavones of G.pentaphyllum were extracted by using methanol and the HPLC fingerprint analysis on an HPLC/DAD system was developed.The signal of chromatogram was decomposed into components at different resolution by wavelet transform.Furthermore,the fractal dimensions of these components were computed for similarity calculation.RESULTS:With the optimized extraction and analytic tool,the fingerprint of G.pentaphyllum was developed.The similarity was effective in evaluating the quality of G.pentaphyllum.CONCLUSION:To describe a fingerprint profile by factors based on fractal and wavelet transform was a more objective method.G.pentaphyllum from Zhejiang province can be used as a substituent for raw drug from Ankang,Shanxi province.