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1.
Psicol. reflex. crit ; 35: 5, 2022. tab
Article in English | LILACS, INDEXPSI | ID: biblio-1365277

ABSTRACT

Teamwork skills are considered essential for personal, academic and professional achievement, so universities are increasingly integrating them into their syllabuses. However, little is known about how some specific features of students and their educational development can affect their acquisition. Accordingly, this study aims to fill this gap and describe higher education students' mastery of teamwork skills and its relation to certain socio-academic variables (gender, academic year and grade point average—GPA). With the aim of determining the level of teamwork skills among university students, an observational, transversal descriptive study was designed with an intentional sample of Spanish university students. The sample is made up of 615 social science degree students. The results suggest significant gender differences, highlighting that female students outdid their male counterparts in most teamwork skills, except leadership. Likewise, students' skills improved as they progressed in their studies, particularly those skills related to adaptability and decision-making. Finally, a positive relationship was observed between teamwork skills and GPA, except for interpersonal development. A regression analysis confirmed the influence of both academic year and GPA for women whilst no effect was detected in the case of men. Based on these results, it is suggested to make changes in university education programmes to compensate for the influence of socio-academic factors and benefit from the most positive features of each gender regarding teamwork to achieve an equal and fair higher education. (AU)


Subject(s)
Students/psychology , Cooperative Behavior , Social Skills , Spain , Universities , Sex Factors , Cross-Sectional Studies , Academic Performance
2.
Yonsei Medical Journal ; : 454-460, 2019.
Article in English | WPRIM | ID: wpr-742560

ABSTRACT

PURPOSE: The present study investigated chest computed tomography (CT) patterns and lung histological features, as well as the consistency between radiological and histological features among patients with microscopic polyangiitis (MPA), granulomatosis with polyangiitis (GPA), and eosinophilic GPA (EGPA). MATERIALS AND METHODS: The medical records of 74 antineutrophil cytoplasmic antibody-associated vasculitis (AAV) patients with radiological lung parenchymal lesions were reviewed along with the histological results for 28 of them. Chest CT patterns were divided according 12 items mostly suggested by radiologists and histological features were divided according to necrotising granuloma, necrotising vasculitis, eosinophilic infiltration, and hemosiderin laden macrophages as defined by a pathologist. RESULTS: The mean age was 57.1 years (22 men). The most common clinical manifestation other than lung manifestation was renal manifestation (62.2%), and the most common chest CT pattern was lung involvement of vasculitis (35.1%). In MPA patients, the major histological features were hemosiderin-laden macrophages in the alveolar space and vasculitis. In GPA patients, the major histological features were necrotizing vasculitis and necrotizing granuloma, while in EGPA patients, the major histological feature was only necrotising vasculitis. The consistency rate in GPA patients was the highest (100%), followed by that in MPA patients (66.7%) and EGPA patients (50.0%). CONCLUSION: When lung involvement of AAV is suspected on chest CT, lung biopsy should be recommended for the proper classification of AAV, due to the discordance rate between radiological and histological findings in MPA and EGPA patients, but not GPA patients.


Subject(s)
Humans , Biopsy , Classification , Cytoplasm , Eosinophils , Granuloma , Granulomatosis with Polyangiitis , Hemosiderin , Lung , Macrophages , Medical Records , Microscopic Polyangiitis , Thorax , Tomography, X-Ray Computed , Vasculitis
3.
Innovation ; : 12-16, 2018.
Article in English | WPRIM | ID: wpr-686906

ABSTRACT

@#BACKGROUND. Since the implementation of Pre-medical curriculum the first students have successfully finished their courses. However, formative and summative evaluation of the curriculum hasn’t been done. The purpose of this study is to analyze the correlation between satisfaction level and knowledge acquisition of the students. MATERIAL AND METHODS. The study was completed at School of Bio-medicine of MNUMS and Division of Education and Policy. We have studied correlation of grade point average (GPA), integrated final exam score, and general admission test/entrance examination test scores (Chemistry and Biology test scores) of total of 395 students’. RESULTS. A total of 395 students’ 4 types of test scores have been used in this study. Out of 395 students, 68 (17%) male, 327 (83%) female and 317 (80%) medical and 78 (20%) health sciences pre-medical students. Results have shown that, both general admission test score and university pre-medical GPA had a direct, average line correlation. In other words, students who have had a higher general admission test score; have scored higher GPA scores at university. The comparison of GPA with the integrated final exam score has shown a direct, average line correlation, which proves the validity of the GPA.The highest factor among was the teacher methodology skills scoring 4.0. The lowest factor was teaching facilities and student lesson load scoring 3.7. CONCLUSION. The satisfaction level is higher the average. The student GPA, general admission test score, both have a direct or average line correlation.

4.
rev. psicogente ; 20(38): 336-352, jul.-dic. 2017. tab
Article in Spanish | LILACS | ID: biblio-963565

ABSTRACT

Resumen Objetivo: Comparar las dimensiones del Síndrome de Burnout en estudiantes de Ingenierías y Ciencias de la Salud, en relación con las variables académicas (promedio ponderado, semestre y programa cursado) y sociodemográficas (edad, género) de los participantes. Método: Dicho estu dio se abordó bajo el enfoque de Bandura (1989), referido a la autoeficacia, bajo el modelo del espiral negativo "hacia abajo", desarrollado por Salanova, Bresó y Schaufeli (2005). Para ello, se desarrolló una investigación de paradigma empírico-analítico, de tipo descriptivo comparativo y diseño transversal. La población estuvo constituida por 1.028 estudiantes de los cuales se obtuvo una muestra estratificada de 280 de ambas facultades participantes; con un porcentaje de error del 5 % y un nivel de confianza del 95 %. A ellos se les aplicó el Inventario de Burnout Académi co (MBI-SS), validado para este estudio. Luego de obtener los resultados de la prueba, se utilizó el estadístico ANOVA para el respectivo análisis de varianzas por grupos. Resultados: Indicaron un nivel bajo y medio de agotamiento emocional y cinismo, pero con un nivel alto de autoeficacia, con diferencias significativas en autoeficacia con respecto al promedio académico y semestre, y de agotamiento con respecto al semestre, programa y facultad; siendo los estudiantes de Odon tología, Medicina, Ingeniería Ambiental y de Sistemas quienes presentaron mayor agotamiento emocional, físico y mental que los otros. Se concluye que existen grupos de estudiantes con diferentes niveles de agotamiento emocional y cinismo, según el género y la carrera que cursan.


Abstract Objective: Comparison of the Burnout syndrome approach, taking into account academic vari ables such as: average, semester and program completion and also demographic variables such as: age and gender in students in the engineering and health sciences programs. This study is cen tered on self-efficacy according to Bandura's theory (1989) and the "downward negative spiral" model developed by Salanova, Breso y Schaufeli (2005). For this purpose, a paradigm of empirical comparative descriptive and cross sectional research is developed. 1,028 students Method: 280 students were a stratified sample; who belong to the two participant programs; 5 % percent level (the margin of error) with 95 % percent level/a confidence level). Academic Burnout Syndrome inventory (MBI-SS) was applied to those students, and it was validated for this study. ANOVA statistical test for the respective variance analysis by groups was used in order to get the results. Results: results showed a low and medium level of emotional exhaustion and cynicism but a high level of self-efficacy with significant differences in relation to semester, grade average, exhausting semester, program and faculty were evident. Students from dentistry, medicine, environmental, engineering and system program and faculty showed greater physical and mental - emotional exhaustion than the others. It is concluded that emotional exhaustion and cynicism level is differ ent according to gender and career path among the student groups.

5.
Innovation ; : 18-23, 2017.
Article in English | WPRIM | ID: wpr-686821

ABSTRACT

@#BACKGROUND As a Mongolian National University of Medical Sciences student rating point is calculated by transferring into 42 level quantitative point in accordance with procedure which assess student approved byDirector with 1st attachment of order no. A/144 on December 15, 2015. However, the calculation,estimated by mathematics allocation, of the student rating point, letter point and quantitative is notbased on research also allocation of transferring the rating point into quantitative point is different foranother universities. These reasons mentioned previously have seriously become basis backgroundof our research. The main objective of this research is to compare model of transferring rating pointinto quantitative point. METHODS Research was completed by quantitative method that based on moment descriptive study, case study, model of mathematic and geometry modeling.Under our research objective we did compare study using rate point that transfer into quantitativepoint of student at MNUHS, accuracy and methodology all among the Mongolian state and private universities. Now Mongolian National University of Life Sciences, Etugen University and New Mongolia Medical University are using 5 level table that approved Ministry of Education Science, Mongolian National University is using 9 level scale, Mongolian University of Science & Technology, Mongolian State University of Education, Mongolian State University of Art&Culture are using 12 level scale while MNUHS is using 42 scale table that calculate student average point. We compared model of GPA calculation that rate point transfer into quantitative point by using all tables belong each Universities mentioned above. We have developed mathematical and geometrical modeling on each assessment since we started study and made statistical process based on modeling. RESULTS Some articles of the regulation to adapt the credit hours, evaluate students’ knowledge and ability during the training where providing the higher educational degree were not fulfilled. Hereto: The article of the guidance that are taking: 70 rating point considering to the letter D, 80 rating point considering to the letter C, 90 rating point considering to the letter B are not providing the 12 level module. The modules with staging 9, 12, 42 of the article on regarding measurement of 2, 3, 4 in 70, 80, 90 rating grade are not meeting requirements. According to the survey among 470 students who’s GPA higher 2.5 in the 42 level scale shows mostly. Also, the percentage of the students with GPA higher than 3 are in high level. In other words, 158 students (33.6% of 470 students) are meeting requirements of the national scholarship programs and other scholarships. The calculation of the correlation relationship of the rating point and grades converted to the digit rate that are using in all universities were the positive correlation, linear dependence. For the module with staging Person Correlation 9, its influence rate is 98.7 per cent that shows the highest rate comparing with other modules. To calculate Determination Percentage (r2), the module with staging 9 is 97.4 per cent or these digit grades have the highest capacity to show the rating grade. CONCLUSION Level models are able to rate in points or statistical significant. The high correlation between rating point and digit grade’s and determination percentage in the 9 level module showing more relevant in statistics and reporting capacity. The modules with staging 9, 12, 42 are not meeting requirements of the guidance.

6.
Rev. colomb. psicol ; 25(1): 63-82, ene.-jun. 2016. ilus, tab
Article in Spanish | LILACS | ID: lil-783631

ABSTRACT

El objetivo de este estudio fue analizar las variables que influyen en el aprovechamiento escolar de los jóvenes. Se configuró una batería de pruebas para medir autoeficacia, autodeterminación, autorregulación, aspectos del contexto escolar y familiar, y espiritualidad. La muestra fue de 250 estudiantes regulares de nivel medio superior. Se estimaron estadísticas univariadas, se calcularon índices y se probó un modelo de ecuaciones estructurales en el que se formaron 2 factores: desarrollo positivo del joven y recurso institucional familiar. La variable de contexto escolar tuvo una influencia directa en el factor familiar y este, a su vez, en el desarrollo positivo del joven, el cual afectó directamente al promedio de los alumnos.


The objective of this study was to analyze the variables that influence the school achievement of young people. A battery of tests was organized to measure self-efficacy, self-determination, self-regulation, aspects of the school and family context, and spirituality. The sample consisted of 250 regular students on the middle school level. Univariate statistics were estimated, indexes were calculated and a model of structural equations was tested in which 2 factors emerged: positive development of the youngster and family institutional resource. The school context directly influenced the family factor and this, in turn, influenced the positive development of youngsters, which directly affected students' GPA.


O objetivo deste estudo foi analisar as variáveis que influenciam no aproveitamento escolar dos jovens. Configurou-se uma série de provas para medir autoeficácia, autodeterminação, autorregulação, aspectos do contexto escolar e familiar, e espiritualidade. A amostra foi de 250 estudantes regulares de nível médio superior. Foram estimadas estatísticas univariadas; calcularam-se índices e provou-se um modelo de equações estruturais no qual se formaram dois fatores: desenvolvimento positivo do jovem e recurso institucional familiar. A variável de contexto escolar teve uma influência direta no fator familiar e este, por sua vez, no desenvolvimento positivo do jovem, o que afetou diretamente a média dos alunos.

7.
Mongolian Pharmacy and Pharmacology ; : 16-19, 2014.
Article in English | WPRIM | ID: wpr-1003343

ABSTRACT

Introduction: Competitiveness is inseparable linked to the quality of teaching in higher education. Therefore, the higher education institutions in many countries around the world to improve international competitiveness in education and special programs to strengthen and develop and implement a successful education policy. Between 1992 and 2012, the number of higher education institutions has increased rapidly in Mongolia. At this time seventeen institutions of higher education increased to 74, and 72 percent has private universities. By 2012, the total number of students increased to 175128, of which 88.7% are enrolled in a bachelor’s degree. In health science, 17575 medical students, of which 16169 are enrolled bachelor’s degree. When we discuss about educational service issues, It is important to consider enrollment, teaching and alumni. To determine the applicant’s quality, it is measured mainly by the university of enrollment examination score of high school, which is measuring the school’s reputation and quality. In recent years, the number of universities or institutes are increased, the number of secondary school graduates were decreased, According to government policy, the strategy is expected to reduce the number of private and state universities or institutes. It shows that it is necessary to study the competitiveness of “Monos” institutes. The purpose of this study is analyzing the student’s learning quality who is studying in “Monos” institute and determining between their general enrollment examination scores and grade point average (GPA) relationship. Materials and Methods: In this survey, included 2010 - 2014 academic year general enrollment examination score and GPA of students learning pharmacist specialty in bachelor degree, used these documents performed mathematical statistical methods using Microsoft excel and SPSS-16 software. Result: It shows a comparison GPA and general enrollment examination score of “Monos” institute’s students which is including from second year’s to fifth year’s. The students GPA and general enrollment examination score has a correlation as measured by the Pearson correlation coefficient which means statistically significant dependence. Second year student’s correlation of GPA and general enrollment examination score (r = 0.3, p = 0.01), junior’s (r = 0.3, p = 0.02), fourth year students (r = 0.3, p = 0.09), senior’s (r = 0.3, p = 0.01). Conclusion: 1. The sophomore to senior year students GPA and the relationship between the general enrollment examinations has been considered Pearson correlation coefficients are statistically significant correlations, directly related to. 2. When we compare a general enrollment examination score, students who have above 500 points are possessing 69% for senior’s, fourth year students are possessing 70%, junior’s 89% and second year students are possessing 97%. In summary, applicants quality have improved in recent years, and so high general enrollment exam scored students must be accepted by Universities and Institutes . 3. To compare between second year student’s GPA to senior student‘s, starting to require professional basic lessons in the second year’s, student’s GPA is decreased. Key word: Studying quality, GPA, General enrollment examination

8.
Archives of Orofacial Sciences ; : 34-40, 2014.
Article in English | WPRIM | ID: wpr-628164

ABSTRACT

Apert syndrome is a rare acrocephalosyndactyly syndrome characterised by craniosynostosis, midface hypoplasia and syndactyly of the hands and feet. The majority of cases arise as the result of one of two mutations of the fibroblast growth factor receptor 2 gene (FGFR2). Due to the involvement of both the cranial and the facial sutures, the keystone of the craniofacial skeleton, the sphenoid bone, is affected by the disease process and as a result is dysmorphic. This may significantly affect craniofacial morphology but it is recognised that there are marked variations in this between different affected individuals. This is a retrospective study examining the morphology of the sphenoid bone using three dimensional reconstructions of computed tomography (CT) scan data. Shape analysis was performed using generalised Procrustes analysis and principal component analysis (GPA/PCA). Comparisons were made between the individuals with Apert syndrome and a group of normal individuals, and between the two genotypic groups. The sphenoid bone in those with Apert syndrome showed marked differences in morphology compared to the normal individuals with a restriction in height and increased angulation of the lesser wings; however, there were no consistent differences between the two genotypic groups. It is possible that fronto-orbital advancement (FOA) surgery indirectly releases the sphenoid bone and allows compensatory growth in this direction.


Subject(s)
Acrocephalosyndactylia
9.
Univ. psychol ; 11(2): 535-544, jun.-dic. 2012. tab
Article in Spanish | LILACS-Express | LILACS | ID: lil-669319

ABSTRACT

Se indagó la relación del burnout académico y las estrategias de afrontamiento con el promedio académico de una muestra de 283 universitarios tomados al azar de los programas de psicología y fisioterapia de la jornada diurna de una universidad privada de Barranquilla, quienes respondieron al MBI-SS y a la Escala de Estrategias de Afrontamiento-Modificada. Los resultados mostraron que la mayoría de los estudiantes presentaban bajos niveles de burnout académico, sin embargo se corroboró que el alto Agotamiento y Cinismo, afectan negativamente el promedio académico; mientras que la Autoeficacia ante los estudios, lo favorecía significativamente, por lo que se establece una relación negativa entre burnout y el promedio académico. Dentro de las estrategias de afrontamiento más frecuentemente utilizadas por los estudiantes, se halló la de Solución de Problemas, la cual se asocia con resultados más favorables en el promedio académico.


The academic burnout might be accompanied of inadequate strategies of coping, contributing to the accentuation of difficulties in the GPA or academic performance. Taking into account the implications of these variables in the performance and the dropout rate at university level, is relevant to investigate the relationship of the academic burnout and coping strategies with the academic performance. A correlational study with a sample of 283 students of the programs of Psychology and Physical therapy of the daytime hours of a private university of Barranquilla, who answered to the MBI-SS and to the Scale of Strategies of Coping Modified (EEC-M) was conducted. The results showed that the majority of the students were presenting low levels of academic burnout. Nevertheless, it was corroborated that the high Exhaustion and Cynicism can negatively affect academic performance; on the contrary, the Self-efficacy to studies, favored it significantly; for what it is possible to establish a negative relation between burnout and the academic performance. Inside the strategies more frequently used by the students, it was found that those that use the one of Solution of Problems in university activities tend to obtain more favorable results in their academic performance.

10.
Korean Journal of Medical Education ; : 133-140, 2006.
Article in Korean | WPRIM | ID: wpr-91926

ABSTRACT

PURPOSE: The present study examines the characteristics of medical students' learning styles and their relationships to the GPA. METHODS: One hundred 2nd year medical students took the revised version of the Index of Learning Styles (ILS) by Felder-Silverman, which was computerized. The ILS classifies individual preferences for information processing into 16 categories based on four dichotomous dimension: active/reflective, sensing/intuitive, sequential/global, visual/verbal. The GPA from the previous semester was used. RESULTS: The most frequent learning styles were reflective, sensing, sequential, and visual, covering 25% of the class. For the relationship with the GPA, we compared group means. The sensing students showed significantly higher grades than the intuitive students. Other than this result, there seemed to be no particular relationship between learning styles and GPA. This relationship should be further studied. CONCLUSIONS: In this study, medical students were categorized based on individual differences in information processing. Learning styles are expected to be helpful in designing effective learning strategies and thereby enhancing medical students' performance.


Subject(s)
Humans , Electronic Data Processing , Individuality , Learning , Students, Medical
11.
Korean Journal of Medical Education ; : 25-32, 2004.
Article in Korean | WPRIM | ID: wpr-224402

ABSTRACT

PURPOSE: This study analyzed the correlation between grade point average (GPA) of medical school and the score of Korean Medical Licensing Examination (KMLE). METHODS: This study based on the results of 67th KMLE applicants who graduated from a college of medicine in 2003. We also gathered data of these applicants from the college of medicine: gender, age, type of entrance, GPA of basic medicine, clinical medicine, clinical clerkships and final test scores. We analyzed whether there was discrimination between achievement of KMLE passed and that of KMLE failed, which of variables affected the results of KMLE. RESULTS: 173 applicants passed KMLE among 189. There were significant correlations between basic medicine, clinical medicine, final test score and the score of KMLE (respective p-value; < 0.0001). There were also significant differences of GPA between KMLE passed applicants and failed. Final test scores were the most correlated with those of KMLE. If the GPA of 2nd grade was below 2.5 and the GPA of 3rd year grade was below 2.3, they was a high-risk group for failing KMLE (sensitivity 100%, specificity 90%). CONCLUSION: There were significant correlations between the GPA of medical school and the score of KMLE, and significant differences between KMLE passed applicants and failed. A high-risk group of failing KMLE was the students that the GPA of 2nd grade was below 2.5 and the GPA of 3rd grade was below 2.3.


Subject(s)
Humans , Clinical Medicine , Discrimination, Psychological , Licensure , Schools, Medical , Sensitivity and Specificity
12.
Chinese Journal of Medical Education Research ; (12)2003.
Article in Chinese | WPRIM | ID: wpr-623817

ABSTRACT

This article focuses on the issue of transfer of majors by college students in the advancement of flexible credit system in Chinese universities,in particular the challenges it brings about to the administration and management of the school.The author also explores the issues occurring under the current administration framework and evaluation mechanism of Chinese universities.

13.
Yonsei Medical Journal ; : 181-186, 2003.
Article in English | WPRIM | ID: wpr-73211

ABSTRACT

This study was undertaken to establish a noninvasive prenatal genetic diagnostic method for trisomy 21 using the fetal nRBCs that is rarely present in maternal circulation. Peripheral venous blood samples were collected from 30 women with an advanced maternal age, abnormal triple marker test results, or abnormal ultrasound findings such as an increased nuchal translucency. The blood samples were treated with heparin. The triple density gradient centrifugation, and MACS using CD45 and CD71 were used to isolate the fetal cells. FISH analysis using probe 21 was performed with GPA-immunostaining. The study population consisted of 30 patients from 13 to 25 weeks of gestation, and nRBCs were separated in all cases. In GPA-immuno FISH analysis using probe 21, 3 cases of trisomy 21 were diagnosed and these results were confirmed by the amniocentesis. In conclusion, a prenatal diagnosis of trisomy 21 through GPA- immuno fluorescence in situ hybridization (FISH) analysis using separated fetal nRBCs is a useful, innovative, accurate, rapid and non-invasive diagnostic method.


Subject(s)
Adolescent , Adult , Female , Humans , Down Syndrome/diagnosis , Immunohistochemistry , In Situ Hybridization, Fluorescence , Pregnancy/blood , Prenatal Diagnosis/methods
14.
Korean Journal of Obstetrics and Gynecology ; : 302-311, 2001.
Article in Korean | WPRIM | ID: wpr-213817

ABSTRACT

INTRODUCTION: Down's syndrome is the most common congenital chromosomal anomalies which occurs 1 out of 700-1000 births. Until now, for prenatal diagnosis of Trisomy 21, invasive techniques such as amniocentesis, chorionic villus sampling(CVS) and cordocentesis were used, but they encompass the rare possibility of morbidity to the mother and fetus. Triple marker using maternal serum is a currently used noninvasive method, but it only shows the accuracy of 60%. Accordingly, a noninvasive method, using fetal cells from maternal blood is under extensive investigation. This study was undertaken to establish a noninvasive prenatal genetic diagnostic method of trisomy 21 using fetal nRBCs rarely present in maternal circulation. MATERIALS AND METHODS: Peripheral venous blood samples were collected from 76 women and treated by heparin. For the isolation of fetal cells, we used a triple density gradient centrifugation, and Vario-MACS and Mini-MACS using CD45 and CD71, and then, the morphological differentiation of the fetal nRBC was performed by Kleihaur-Betke stain. With GPA immunostain, nRBCs were identified by cytoplasm and GPA attatchment, and after marking the site, a FISH was performed. RESULTS: This study population included 76 patients from 8 to 41 weeks of gestation, and nRBC was separated from all cases. The morphological differentiation was achieved by K-B stain. The mean number of nRBC collected from 20 ml of maternal peripheral blood was 15. The number of nRBCs retrieved reached its peak in 12-18 gestational weeks(18.9 6.0) which decreased from 20 gestational weeks and thereafter. Fetal sex was determined by FISH analysis using probe X, Y with GPA-immunostained cells. GPA-immuno FISH analysis using probe 21 in 30 cases of advanced maternal age or positive triple markers, we confirmed 3 cases of Down's syndrome. These results were also confirmed using the CVS or amniocentesis. CONCLUSIONS: Fetal nRBCs were separated from all cases after 8 gestational weeks. Prenatal diagnosis of trisomy 21 through GPA-immuno FISH analysis of chromosome 21 using separated fetal nRBCs is an useful, innovative, accurate, rapid and non-invasive diagnostic method. But for clinical use, more cases of experiments will be needed.


Subject(s)
Female , Humans , Pregnancy , Amniocentesis , Centrifugation, Density Gradient , Chorionic Villi , Chromosomes, Human, Pair 21 , Cordocentesis , Cytoplasm , Down Syndrome , Fetus , Heparin , In Situ Hybridization , Maternal Age , Mothers , Parturition , Prenatal Diagnosis , Trisomy
15.
Yonsei Medical Journal ; : 258-265, 2000.
Article in English | WPRIM | ID: wpr-74160

ABSTRACT

Fetal nucleated red blood cells (nRBCs) are rare in maternal circulation, but their presence constitutes a potential source of non-invasive prenatal genetic diagnosis. This study was undertaken to establish a non-invasive prenatal genetic diagnosis method using isolated fetal nRBCs. A multi-step method including triple density gradient and magnetic activated cell sorting (MACS) using CD45 and CD71, cytospin centrifugation, K-B staining, and glycophorin A-immuno fluorescence in situ hybridization (GPA-immuno FISH) was performed. The study population included 65 patients from 8 to 41 weeks of gestation, and fetal nRBC was separated from all cases. The number of fetal nRBCs retrieved was 12.8 +/- 2.7 in 8 to 11 gestational weeks, 15.2 +/- 6.5 in 12 to 18 gestational weeks, 16.4 +/- 6.5 in 19 to 23 gestational weeks, 10.6 +/- 3.2 in 24 to 28 gestational weeks, and 5.5 +/- 1.9 in 35 to 41 gestational weeks: the mean number of nRBCs collected from 20 ml of maternal peripheral blood was 13.7 +/- 6.2. The highest value of yield was 45.6% from 12 to 18 weeks gestation. The fetal sex determination confirmed by amniocentesis or chorionic villus sampling showed 100% sensitivity and 91.7% specificity for males; 91.7% sensitivity and 100% specificity for females. We showed that fetal cells can be reliably enriched from maternal blood and that they can be used for detecting specific chromosomes by FISH with a specificity superior to current non-invasive methods.


Subject(s)
Female , Humans , Pregnancy , Erythrocytes/immunology , Fetal Blood/immunology , Gestational Age , Glycophorins , Immunomagnetic Separation , Immunophenotyping , In Situ Hybridization, Fluorescence , Prenatal Diagnosis
16.
Chinese Journal of Marine Drugs ; (6)1994.
Article in Chinese | WPRIM | ID: wpr-594078

ABSTRACT

Objective To study on glutathione and glutathione-related enzymes of Antarctic ice microalgae.Methods Glutathione(GSH) content,glutathione produce ability(GPA) and glutathione reductase(GR) activity in 24 kinds of Antarctic ice microalgae were assessed using the spectrophotometer methods.Results GSH content of Cyanophyceae B-1 was highest.Total GSH yields of Antarctic ice microalgae Chlamydomonas sp.ICE-L and Berkeleya rutilans GJ01 were in the top two positions.GPA in most Antarctic ice microalgae was higher than that in mesophilic green algae,and only in two ice microalgae was lower than that in Phaeodactylum tricornutum.GR activities of Antarctic ice microalgae Berkeleya rutilans GJ01 and Chlamydomonas sp.ICE-L were greater than that of the control microalgae.Conclusion It is possible that Antarctic ice microalgae will become a new resource of GSH.

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