ABSTRACT
Objective:To investigate the expression of hypoxia-inducible factor-2α (HIF-2α)in non-small cell lung cancer (NSCLC)tissue,and to analyze its relationships with angiogenesis,cell proliferation and chemotherapy resistance. Methods: Total 112 cases of NSCLC and 20 cases of normal lung tissues were selected, immunohistochemical method was used to detect the expressions of HIF-2α,CD31,Ki67 and GST-π in 112 cases of cancer tissue and 20 cases of normal lung tissue,and the correlations of HIF-2α expression with microvessel density (MVD),Ki67, and GST-π were analyzed.Results:The positive expression rate of HIF-2α in NSCLC tissue was significantly higher than that in normal lung tissue (P < 0.05 ), the expression rate of HIF-2α in 112 cases of NSCLC was 47.3% (53/112).The MVD in HIF-2α protein high expression NSCLC group (31.1 ± 14.7)was higher than that in HIF-2αprotein low expression NSCLC group (24.3±15.8)(P <0.05).The cases of high expression of Ki67 in HIF-2αhigh expression group occupied 54.7% (29/53),and it was higher than that in HIF-2αlow expression group (16/59,27.1%);there was significant difference (r = 0.281,P = 0.003).The high expression of HIF-2α had no obvious correlation with the expression of GST-π (r = 0.122,P = 0.202). Conclusion:HIF-2αmay play an important role in the carcinogenesis and development of NSCLC by promoting the angiogenesis and enhancing the cell proliferation of NSCLC,but it may have no correlation with chemotherapy resistance.
ABSTRACT
10.3969/j.issn.1000-8179.2013.12.001
ABSTRACT
Previous studies have suggested that glutathione-S-transferase π (GST-π) over-expression in the brain tissue is associated with refractory epilepsy.However,whether the change in GST-π level in the peripheral blood is in line with that in brain tissue remains unknown.This study examined the correlation between GST-π in brain tissue and that in peripheral blood in rat models of pilocarpine-induced refractory epilepsy.The animals were divided into drug-resistant group and drug-responsive group according to the response to anti-epileptic drugs.GST-π expression in brain tissue was immunohistochemically determined,while the expression of GST-π in peripheral blood was analyzed by Western blotting.In the hippocampus and cortex,GST-π was mainly found in the cytoplasm and membrane of neurons,and the GST-π expression level was higher in drug-resistant group than in the drug-responsive group and saline control group (P＜0.05).Moreover,there was no significant difference between responders and saline control animals (P＞0.05).The change in expression of GST-π in peripheral blood showed the same pattern as that in brain tissues,suggesting GST-π might contribute to drug resistance in epilepsy.Importantly,the GST-π over-expression in peripheral blood could be used as a marker for resistance to anti-epileptic agents.