ABSTRACT
Objective: To study the therapeutical effect of Jiuwei Gantai Capsule (JWGT) on nonalcoholic fatty liver disease (NAFLD) of rats induced by high fat diet and its possible therapeutic mechanism. Methods: The experimental rats were divided into six groups: normal group, model group, silymarin group (0.05 g/kg), JWGT high-, mid- and low-dose groups (1.80, 0.90, and 0.45 g/kg). Except the normal group, NAFLD rats were fed with high fat diet for 10 weeks, those other groups were ig given relevant medicine from the week 5. All the rats were put to death after 10 weeks, the changes of body weight, liver weight, liver index, and hepatic histopathological morphology were observed. The high density lipoprotein cholesterol (HDL-C), free fatty acid (FFA), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in serum were collected for the contents detection. Total choesterol (TC), triacylglycerol (TG), superoxide dismutase (SOD), and malondialdehyde (MDA) in hepatic tissue were detected, too. Results: Compared with the model group, the content of FFA, AST, and ALT in serum of NAFLD rats were decreased (P < 0.05), while HDL-C was obviously improved (P < 0.05) in JWGT high- and mid-dose groups. The TC, TG, and MDA in the hepatic tissue were obviously decreased (P < 0.05), while the activity of SOD was significantly improved (P < 0.05). Meanwhile, the pathological images of hepatic tissue and classified graded method of steatosis were improved in NAFLD rats. Conclusion: JWGT has the regulation of lipid and protection of liver for NAFLD rats. Its therapeutic mechanism is partly related to regulating lipid metabolism and anti-oxidative damage.
ABSTRACT
OBJECTIVE:To establish an HPLC method for the assaying of silybin in Gantai capsule.METHODS:The assay was performed on Diamonsil C18 column.The mobile phase consisted of potassium dihydrogen phosphate solution-methanol(45:55)with a flow rate at 1.0ml/min,detection wavelength at 288nm,column temperature at(25?2)℃.The sample size was 20?l and the internal standard was kaempferol.RESULTS:There was a good linear relationship within the concentration range of 0.126～1.26 mg/ml(r=0.9 998)for silybin.The average recovery was 102.9%(RSD=1.50%).CONCLUSION:The method was simple,accurate and reproducible,and suitable for the assaying of silybin in Gantai capsule.