Human Peripheral Blood Mononuclear Cell Proteome Analysis of Parkinson Disease with Different TCM Syndromes / 中国中医药信息杂志

Objective To investigate the comparative proteomes difference of PBMC between health adults and Parkinson Disease(PD) patients with Ganyang Huafeng syndrome and Xuexu Shengfeng syndrome.So to approach the essence of the two TCM syndromes from the protein expression level.Methods A series of methods,including immobilolized pH gradient two-dimensional polyacrylamide gel electrophpresis(2-DE),Coomassie Brilliant Blue staining,PDQuest 2-DE analysis software,peptide massfingerprint based on matrix-assisted laser desorption/ionization time of flying mass spectrometry(MALDI-TOF-MS) and SWISS-PROT database searching,were used to separate and indentify the proteome of PBMC between health adults and PD patients with Ganyang Huafeng syndrome and Xuexu Shengfeng syndrome.Results The good 2-DE pattern including resolution and reproducibility was obtained.After Coomassie Brilliant Blue staining,15 spots were incised and analyzed by MALDI-TOF-MS.The typic peptide masses were searched in the SWISS-PROT database by Mascot software.All proteins were preliminarily identified,which ralated to cytoskeleton,anti-oxidative stress,protein degradation,signal transduction and cell cycle,etc.Conclusions The well-resolved,reproducible 2-DE patterns of Ganyang Huafeng syndrome and Xuexu Shengfeng syndrome PD were established and revealed the protein expression difference of PBMC between the two syndromes of PD patients.This research is helpful to provide substance evidence in investigation of the essence of Ganyang Huafeng syndrome and Xuexu Shengfeng syndrome on protein level.

Proteromics Analysis and Identification on Peripheral Blood Lymphocyte of Ganyang Huafeng Syndrome of Cerebral Infarction / 中国中医药信息杂志

Objective To analyze and identify the proteins relevant to Ganyang Huafeng Syndrome(GYHF) and Yinxu Shengfeng Syndrome(YXSF) of cerebral infarction,and investigate the essence of GYHF and YXSF.Methods 3 groups,GYHF of cerebral infarction,YXSF of cerebral infarction and healthy people were designed with 15 cases of each group.Total protein were extracted from lymphocyte of peripheral venous blood,and two dimensional electrophoresis was used to separate total proteins from norma land model ratsynovium.Having been stained,proteins were analyzed by PDquest software.Some selected protein spots were identified by peptide mass fingerprint(PMF) based on Matrix assisted laser desorption/ ionizationtime of flight mass spectrometry(MALDI-TOF-MS) and database.Results 23 different protein spots and 8 identical peotein spots were found out from GYHF and YXSF group,22 proteins were identified from the different protein spots including 15 kinds of proteins and 6 protein spots including 5 kinds of proteins.GYHF of cerebral infarction related with proteins such as metabolic enzymes,signal transmission proteins,cytoskeletal protein,antioxydation proteins and so forth.Conclusion GYHF and YXSF of cerebral infarction have different proteins and identical proteins,which indicates that the two syndromes have the same matter substructure and different essential connotation.