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Objective To investigate the effects of garlicin capsule on immune function in patients with breast cancer undergoing chemotherapy??Methods Form April 2018 to October 2018,62 patients with breast cancer were treated by operation in Tangshan People′s Hospital and pathologically diagnosed as breast cancer??Random number table method was used to divide the patients into treatment group and control group, with 31 cases in each group??The treatment group was given garlicin capsule combined with EC regimen( the first day( epirubicin hydrochloride injection 90 mg／m2+ cyclophosphamide 90 mg／m2 ) ,21 days for one cycle,four consecutive cycles), while the control group was given EC regimen alone for 4 courses??The occurrence of adverse reactions was recorded??T lymphocyte subsets were detected before and after treatment to evaluate the immune function of the body??Results There was no significant difference in the number of adverse reactions between the two groups after treatment ( Seventy in the treatment group and 69 in the control group) ( P＞0??05)??The levels of CD3+, CD4+, CD8+ and CD4+／CD8+ were ( 53??76 ± 4??29)%, (40??19 ±3??71)%,( 28??61 ± 2??75)% and ( 1??41 ± 0??16) in the treatment group before chemotherapy, respectively??While after chemotherapy were ( 52??41 ± 4??44)%, ( 39??49 ± 4??06)%, ( 28??70 ± 2??76)% and (1??35± 0??94), respectively??The levels of CD3+, CD4+, CD8+ and CD4+／CD8+ were ( 54??35 ± 4??09)%, (40??70± 4??09 )%, ( 27??62 ± 3??60 )% and ( 1??49 ± 0??24 ) in the control group before chemotherapy, respectively??While after chemotherapy were ( 44??27 ± 6??67)%, ( 35??39 ± 4??96)%, ( 30??48 ± 3??43)% and (1??17±0??20)??After treatment,the levels of CD3+,CD4+,CD4+／ CD8+ decreased and CD8+ increased,but there had no significant difference( P＞0??05)??After treatment,CD3+( decreased from( 54??35 ± 4??09)% to (44??27 ± 6??67 )%), CD4+( decreased from ( 40??70 ± 4??09 )% to ( 35??39 ± 4??96 )%), CD4+／CD8+ ((decreased from (1??49±0??24) to (1??17±0??20)),the difference was statistically significant( t value was 7??17,4??60,5??71,－3??02,P value was 0??000,0??000,0??002,0??000)??Compared with control group,the levels of CD3+、CD4+、CD4+／ CD8+in treatment group increased and CD8+decreased significantly (all P＜0??01)??Conclusion Garlicin capsule can improve the depression of immune function caused by chemotherapy??
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OBJECTIVES@#To evaluate whether garlicin post-conditioning can attenuate myocardial ischemiareperfusion injury in a catheter-based porcine model of acute myocardial infarction (AMI) by affecting adhesion molecules integrin β1/CD29 and platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31).@*METHODS@#Twenty-two swine were devided into 3 groups: 6 in a sham-operation group, and 8 each in the model and garlicin groups. AMI porcine model was established in the model and garlicin groups. The distal parts of the left anterior descending coronary artery in the animals of the model and garlicin groups were occluded by dilated balloon for 2 h, followed by reperfusion for 3 h. Garlicin (1.88 mg/kg) was injected over a period of 1 h, beginning just before reperfusion, in the garlicin group. Real-time polymerase chain reaction, immunohistochemistry and Western blot were carried out to detect mRNA and protein expressions of CD29 and CD31 3 h after reperfusion.@*RESULTS@#Hematoxylin-eosin staining showed a better myocardial structure in the garlicin group after reperfusion. Compared to the model group, garlicin inhibited both the mRNA and protein expression of CD29 and CD31 in reperfusion area and no-reflflow area (P<0.05 respectively).@*CONCLUSIONS@#Garlicin post-conditioning induced cardio-protection against myocardial ischemia-reperfusion injury in this catheter-based porcine model of AMI. The cardio-protective effect of garlicin is possibly owing to suppression of production of CD29 and CD31, by inhibition of the mRNA expression of CD29 and CD31.
Subject(s)
Animals , Male , Allyl Compounds , Pharmacology , Disease Models, Animal , Disulfides , Pharmacology , Integrin beta1 , Genetics , Physiology , Ischemic Postconditioning , Myocardial Reperfusion Injury , Platelet Endothelial Cell Adhesion Molecule-1 , Genetics , RNA, Messenger , SwineABSTRACT
Objective To study the protective effect of garlicin on radiation injury in mice. Methods Sixty mice were randomly divided into five groups:model control group, solvent control group and garlicin treatment groups[0.17, 0.33, 1.00 g/(kg?d)]. After treated by gavage for 20 consecutive days, the mice were exposed to 60Co-? irradiation for one time and the effect of the irradiation on the amounts of WBC in peripheral blood, the ratio of micronucleus cells in marrow, the content of DNA in marrow cells and the superoxide dismutase (SOD) activity were measured. Results Compared with the exposure group, garlicin increased the amounts of WBC, decreased the micronucleus frequency of marrow cells, increased the content of DNA in marrow cells and increased the SOD activity in mice. Conclusion Garlicin shows a protective effect on irradiation injury in mice.
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AIM: To study the effect of garlicin on protecting nonalcoholic fat liver in rats induced by high fat diet and explore the pathogenesis involved. METHODS: According to the dosage of garlicin and diet, fifty SD rats were randomly divided into five equal groups: normal control group, model control group and garlicin (10, 20, 30 mg/kg) groups. Apart from the rats in normal control group, the rats were all fed with high fat and high cholesterol diet. After 12 weeks, the levels of serum endotoxin (ETX), total cholesterols (TC), triglyceride (TG), superoxide dismutase (SOD), malondialdehyde (MDA), transaminase and free fatty acids (FFA) were detected. The levels of MDA, SOD, glutathione hormone (GSH) in hepatic tissue were also detected. Then the features of live pathology were observed. RESULTS: The levels of ETX, TC and TG in garlicin groups were significantly lower than those in model control group (P
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Objective To assess the therapeutic effect of garlicin on Pneumocystis carinii pneumonia (PCP) of immuosuppressed rats. Methods The Wistar rats were injected intramuscularly continually with dexamethasone for eight weeks to establish the rat model of PCP. The rats were treated with garlicin, meanwhile control groups without treatment and with SMZ-TMP treatment were established respectively in PCP model. The efficacy was evaluated based on the lung weight, lung /body weight ratio and mean cyst number per 100 fields in lung print smear. Results The mean lung weight of the rats in garlicin is 1.73?0.17, lung/body weight ratio is 0.84?0.12, they are obvious lower than 2.31?0.35 and 1.25?0.35 of control (P