ABSTRACT
Objective To establish a method for quantitative determination of 17 kinds of organophosphates pesticides in vegeta‐bles by gas chromatography and mass spectrometry (GC‐MS) .Methods The sample was extracted by acetonitrile ,purified by the a‐mino solid phase extraction small column ,concentrated ,detected by GC‐MS .Then the ion scanning (SIM ) mode was selected to mo‐nitor the specific iron in target compound for conducting the quantitative analysis .Results 17 kinds of compound had good linearity in the limit of detection (LOD) of 0 .001 - 0 .02 mg /kg (S/N = 3) with a relative standard deviation of 1 .05% - 6 .81% ,and the re‐covery rate was 66 .4% - 99 .3% when with cap at 004 ,008 ,0 .40 mg/kg .Conclusion This method has good selection and high sensitivity ,and is suitable for the rapid determination of organophosphates pesticide residues in vegetables .
ABSTRACT
Background Trans-arachidonic acids (TAAs) are specific lipid markers of nitrative stress and the major products of NO2·-mediated ismoerization of aracchidonic acid(AA),and they represent a possible new mechanism of NO2·-induced toxicity in ischemic retinopathy.Our previous study demonstrated that high glucose induce nitrative stress response. Objective This study aimed to evaluate the effect of nitrative stress on retinal capillary in diabetic rats by detecting the TAAs with gas chromatography and mass spectrometry(GC/MS).Methods One hundred 2-week-old clean male SD rats were randomly divided into control group and diabetic group.Diabetic rat models were induced by intraperitoneal injection of streptozoticin of 60 mg/kg,and the successful models were identified as the blood glucose level>16.7 mmol/L.The synthesis method was modified in this study andaraehidonic acid w8$utilized as starting material.14E-AA was obtained as a standard sample via expoxidation and deoxidation in a safe and practical pattern.Serum TAAs and AA in diabetic rats were detected by GC/MS in 2,4,8,12 and 16 weeks after injection of streptozoticin.The ratio of peak area of TAAs to AA with selected ion of 79 was estimated and compared with normal rats. Results Fifty rats had been in Diabetic models were established in 50 rats with the successful rate 100%,and the high blood glucose level in model rats retained throughout the experimental duration.GC/MS results showed that the 14E-AA was obtained with the purity of 97.3%,which provided a standard sample to nitrative stress-related research as a premise. No significant differences were found in serum TAAs/AA values at 2 weeks and 4 weeks between model group and control group (t =-0. 376, t =-0. 642,P>0. 05). However,serum TAAs/AA values in at 8,12 and 16 weeks after streptozoticin injection were considerably elevated in comparison with those of control group (0. 0832 vs 0. 1042,0. 0910 vs 0. 1568, 0. 1100 vs 0. 1895;t=-36.409,t=-166.714,t=-148.212,P<0. 05). Conclusion Elevation of serum TAAs/AA is associated with diabetic duration,implying that damage of nitrative stress to retinal capillary is a parallel procedure to diabetic course.Establishment of GC/MS detection system offers a new evaluating indicator in the research on microvascular ischemic disease and could be implemented in clinical testing.
ABSTRACT
To investigate the quality of different parts of Radix Fici Hirtae. The content and components of volatile oils and ethanol-extracts from different parts of Radix Fici Hirtae were analyzed by the combination of gas chromatography and mass spectrometry (GC-MS). The content of volatile oils in the cortex was higher than that in the xylem and the chemical components either in the cortex or in the xylem are similar. The main components in volatile oils are hexadecanoic acid, oleic acid+linoleic acid, linoleic acid amide, palmitic acid amide,stearic acid amide and dibutyl phthalate. The main components in ethanol-extracts are psoralen, hexadecanoic acid, hexadecanoic acid ethyl ester, bergapten, oleic acid and linoleic acid. The content of psoralen in the cortex was higher than that in the xylem.[Conclusion]The chemical components in different parts of Radix Fici Hirtae are similar but the contents of volatile components and psoralen are higher in the cortex than those in the xylem.
ABSTRACT
To compare the chemical components and their contents of essential oil extracted from Radix Angelicae Sinensis by the methods of supercritical CO 2 fluid extraction (SFE-CO 2), normal pressure steam distillation (NPSD) and vacuum steam distillation (VSD). The combination of gas chromatography and mass spectrometry was applied to analyze the chemical components and their contents of essential oil extracted from Radix Angelicae Sinensis.The chemical components and their contents of essential oil extracted by the above three methods were different.[Conclusion]SFE-CO 2 is superior to VSD and NPSD in raising yield and shortening extractive time. It is a good method for the extraction of essential oils from Radix Angelicae Sinensis.