Pharmacodynamic interaction of Boerhaavia diffusa with omeprazole in experimentally induced ulcers in rats .

The study evaluates the possible gastro protective of combination therapy of Omeprazole and Boerhaavia diffusa using three different gastric ulcer models. Gastric Ulcers in SD rats were induced by Indomethacin (25mg/kg), Pyrolus ligation model and stress-induced Ulcer. Various parameters like free acidity and total acidity, ulcer index, ulcer score, pepsin and mucin content, anti oxidant parameters like super oxide dismtase and catalase were evaluated. Omeprazole (2mg/kg) was used as the standard drug. Boerhaavia diffusa was administered at two dose levels, 200mg/kg and 400 mg/kg body weight. Statistical analysis was done by ANOVA followed by Dunnet’s Multiple Comparision test. P<0.05 was considerable statistically significant.Oral administration of combination of Omeprazole and Boerhaevia diffusa at 200 and 400 mg/kg produced significant (p<0.01 & p<0.001) decrease in acidity, ulcer index and severity of ulceration in the pylorus ligation model as well as protection against stress and Indomethacin induced ulcerations compared to control. It also shows significant (p<0.001) decrease pepsin content and significant (p<0.001) increase in mucin content compared to control pylorus ligation model. In Indomethacin induced model combination therapy at high level shows significant increase (p<0.001) in antioxidant parameters like SOD and catalase compared to control. The anti ulcer effects of combination of Omeprazole and Boerhaavia diffusa at both the dose levels were significantly higher than that of omeprazole alone. Combination therapy was found to be an effective anti ulcerogenic agent, minimizing any possible side effects. The result of the study suggests that combination therapy causes an inhibitory effect on release of gastric hydro chloric acid and protects gastric mucosal damage.

In vitro and in vivo anti-Helicobacter pylori activities of FEMY-R7 composed of fucoidan and evening primrose extract / 한국실험동물학회지

Effects of FEMY-R7, composed of fucoidan and evening primrose extract, on the bacterial growth and intragastric infection of Helicobacter pylori as well as gastric secretion were investigated in comparison with a proton-pump inhibitor pantoprazole. For in vitro anti-bacterial activity test, H. pylori (1x10(8) CFU/mL) was incubated with a serially-diluted FEMY-R7 for 3 days. As a result, FEMY-R7 fully inhibited the bacterial growth at 100 microg/mL, which was determined to be a minimal inhibitory concentration. In addition, 6-hour incubation with H. pylori, FEMY-R7 inhibited urease activity in a concentration-dependent manner, showing a median inhibitory concentration of 1,500 microg/mL. In vivo elimination study, male C57BL/6 mice were infected with the bacteria by intragastric inoculation (5x10(9) CFU/mouse) 3 times at 2-day intervals, and simultaneously, orally treated twice a day with 10, 30 or 100 mg/kg FEMY-R7 for 7 days. In Campylobcter-like organism-detection test and bacterial identification, FEMY-R7 exerted a high bacteria-eliminating capacity at 30-100 mg/kg, comparably to 30 mg/kg pantoprazole. In contrast to a strong antacid activity of pantoprazole in a pylorus-ligation study, FEMY-R7 did not significantly affect gastric pH, free HCl, and total acidity, although it significantly decreased fluid volume at a low dose (10 mg/kg). The results indicate that FEMY-R7 eliminate H. pylori from gastric mucosa by directly killing the bacteria and preventing their adhesion and invasion, rather than by inhibiting gastric secretion or mucosal damage.

Inhibitory effects of a beta-dunnione compound MB12662 on gastric secretion and ulcers / 한국실험동물학회지

The effects of a beta-dunnione compound MB12662 on the gastric secretion and ulcers were investigated in rats. In order to assess the effects of MB12662 on the gastric secretion and acidity, rats were subjected to pylorus ligation operation, and 6 hours later, gastric fluid was collected. Treatment with MB12662 reduced the gastric fluid volume to 47.3% of control level and increased pH. In an alcohol-induced ulcer model, rats were orally administered 3 mL/kg of ethanol, and 1 hour later, the ulcer lesions ware measured under a stereomicroscope. MB12662 reduced ulcer index in a dose-dependent manner which was much stronger than a proton-pump inhibitor pantoprazole. In a stress-induced ulcer model, rats were subjected to water-immersion restraint stress, and 5 hours later, the ulcer lesions ware examined. MB12662 also attenuated the stress-induced gastric lesions, although the efficacy of MB12662 was lower than that of pantoprazole. Therefore, it is suggested that MB12662 could be a candidate compound for the prevention or treatment of gastric ulcers induced by gastric over-secretion and alcoholic hangover.

Serie de la fisiología a la clínica. Secreción gástrica e inhibidores de bomba de protones / Series: from physiology to the clinic. Gastric secretion and proton pump inhibitors

La función principal del estómago es de tipo secretoria y digestiva a través del almacenamiento, procesamiento y vaciamiento al intestino de los alimentos ingeridos. La secreción gástrica requiere de una compleja red de interacciones neurales, endocrinas, autocrinas y paracrinas que funcionan como un todo, para lograr un delicado equilibrio fisiológico que permita la digestión y absorción de nutrientes. A su vez, el uso cada vez más difundido y en forma libre de inhibidores de secreción gástrica como los inhibidores de bomba de protones requiere un adecuado conocimiento de la fisiología de la secreción gástrica y de los mecanismos de inhibición de la misma para un uso racional en la práctica clínica diaria.

The principal function of the stomach is secretory and digestion thanks to the capacity of store, process and empties of food to the bowel. The acid secretion needs of complex neural functions, endocrines, autocrine y paracrine that act like a hole. The use of proton pump inhibitors is every day more frequent and over the counter, and requires the knowledge of the normal gastric physiology and the mechanism of inhibition for a rational use in clinical practice.

Effects of Jianpi Xiaopi extract on gastric secretion of spleen deficiency rats / 第三军医大学学报

Objective To observe the effects of Jianpi Xiaopi extract on gastric secretion of experimental SD rats with spleen deficiency. Methods Animal model of spleen deficiency was established by using Rhubarb decoction. The gastric secretion was determined by titration method, and the gastrin in plasma was measured by radioimmunoassay. Results Compared with those in spleen deficiency group, the levels of the total acidity and the output of total acid were higher in the middle dose group treated with Jianpi Xiaopi extract ( P

Effect of Xiangshahewei Pill on secretion in anorectic rat models induced by Fenfluramine / 中成药

AIM:To explore the effect of Xiangshahewei Pill(Radix Aucklandiae,Rhizoma Cyperi,Fructus Amomi,etc.)on secretion in anorectic rat model induced by Fenfluramine.METHODS:Anorectic rat model was induced by Fenfluramine in dose of 15 mg/kg for 8 days.Xiangshahewei Pill was administered orally to the rats also for 8 days.The volume of gastric juice,total gastric acid,mucus,acidity of gastric acid and activity of pepsin of rats were measured.RESULTS:Xiangshahewei Pill could improve the turbulence of gastric secretion in anorectic rat induced by Fenfluramine.Xiangshahewei Pill in dose of 0.75 g/kg(P

Inhibition of gastric secretion by a water extract from Baccharis triptera, Mart

Baccharus triptera Mart, is a widespread Compositae used in Brazilian folk medicine to treat gastrointestinal disturbances, rheumatic disease, mild fever, diabetes and as an anti-helminthic. Water extract of small branches of the plant (WE) administered to mice and rats (0.1 to 2 g/Kg, p.o) did not alter spontaneous motor activity, sleeping time induced by barbiturates or the tailflick response in mice. The extract decreased by 40 por cento the number of writhings induced by 0.8 por cento scetic acid, i.p., but did not influence paw edema induced by carrageenan or dextran in rats WE (2g/Kg, p.o.) decreased the intestinal transit of charcoal in mice by 20//. Gastric secretion in pylorus ligated rats was reduced after treatment with WE (1 and 2 g/Kg. i.p. or intraduodenal and the gastric pH was raised. The extract (1 g/Kg, p.o.) prevented gastric ulcers induced in rats by immobilization at 4ºC, but not those induced by indomethacin (10 mg/Kg, s.c.). The results indicate that WE may relieve gastrointestinal disorders by reducing acid secretion and gastrointestinal hiperactivity. Neither analgesic nor anti-inflammatory activities were detectable. .

The water extract of Coleus barbatus Benth decreases gastric secretion in rats

Coleus barbatus (Labiatae) Benth is popularly used in Brazil "for the healing of liver and stomach diseases". The water extract (WE 1 to 10 g/Kg, p.o.) of stem and leaves given to rats and mice did not induce signs of intoxication. Preveious treatment of mice with WE (1 g/kg, p.o.) shortened the sleeping time induced by pentobarbital (50 mg/Kg, i.p.) by 37 por cento, althoyugh the extract alone did not increase the spontaneous activity nor did it induce hyperexcitability. In mice WE (2 g/Kg, p.o.) increased the intestinal transit of charcoal by 30 por cento, while reduced gastric secretions ion rats treated with WE (2g/Kg intraduodenal) 3,9 ± 1.0 to 0.5 ± 0.2 ml/4h, respectively). The treatment also reduced the total acid secretion from 34.4 ± 11.0 to 2.7 ± 0.5 mEq/l and raisedgastric pH from 2.2 ± 0.3 to 6.5 ± 0.8. Treatment with WE (2g/Kg, p.o.) protected against gastric ulcers induced by stress (5.3 ± 1.6 and 1.5 ± 0.5 ulcers/cm²), but did nor protect against indonethacin induced ulcers. The results show that the water extract of C barbatus Benth produces mild stimulation of thecentral nervous system and increases intestinal movements. The extract also reduces gastric secretion indicating an antidyspeptic activity, and protects against gastric ulcers induced by stress.