Correlation between bizygomatic and maxillary central incisor width for gender identification / Correlação entre a distância bizigomática e a espessura de incisivo central maxilar para identificação de gênero

Objective: The aim of the present study is to analyze and evaluate the applicability of bizygomatic and maxillary central incisor width in identifying the sex of an individual for anthropological studies. Material and Methods: The study was conducted on 100 individuals in a private dental institution. The width of the central incisor was measured by requesting the subject to bite onto a sheet of modelling wax. The bizygomatic width was calculated with the help of a divider by taking the most prominent area of the zygomatic arch as the reference point bilaterally. Berry's formula was used to calculate the width of the maxillary central incisor from the bizygomatic width. Berry's Formula "Width of the maxillary central incisor = Bizygomatic width / 16." The data obtained was tabulated and analyzed statistically. Results: The results in our study indicated that the widths of both maxillary central incisors and the bizygomatic width were found to be higher in males when compared to females with a positive strong correlation. Conclusion: The Berry's index can be used for identifying the gender and can also be used for facial reconstruction. (AU)

Objetivo: O objetivo do presente estudo é analisar e avaliar a aplicabilidade da distância bizigomática e espessura de incisivos centrais maxilares na identificação do sexo de um indivíduo para estudos antropológicos. Material e métodos: O estudo foi conduzido com 100 indivíduos de uma instituição odontológica privada. A espessura do incisivo central foi medida pedindo ao sujeito que mordesse em uma folha de cera. A espessura bizigomática foi calculada com o auxílio de uma régua pegando a área mais proeminente do arco zigomático como ponto de referência bilateralmente. A fórmula de Berry foi usada para calcular a espessura do incisivo central maxilar da espessura zigomática. Fórmula de Berry: "Espessura do incisivo central maxial = Espessura bizigomática / 16". Os dados obtidos foram tabulados e analisados estatisticamente. Resultados: Os resultados em nosso estudo indicaram que as espessuras de ambos os incisivos centrais maxilares como as espessuras bizigomáticas foram maiores no sexo masculino do que no sexo feminino, com uma correlação positiva forte. Conclusão: O índice de Berry pode ser usado para identificação de gênero e também pode ser usado para reconstrução facial. (AU)

Diferencias por género a través del estudio de los tipos de huellas labiales en estudiantes de posgrado de la Facultad de Odontología de la Universidad de Buenos Aires / Gender differences through the study of lip prints types in postdoctoral students from the School of Dentistry, University of Buenos Aires

Las huellas labiales representan una alternativa para la identificación humana por ser únicas en cada persona. Se buscó establecer la frecuencia de tipos de huellas labiales en estudiantes de Posgrado de la Facultad de Odontología de la Universidad de Buenos Aires, tomando como referencia la clasificación de Suzuki y Tsuchihashi. Se realizó un estudio descriptivo en 50 hombres y 50 mujeres. Se fotografiaron los labios de cada participante, aplicando posteriormente lápiz labial a los efectos de que efectúen impresiones sobre un soporte de papel, estableciéndose los diferentes tipos de huellas. Se transcribieron los datos a una ficha diseñada a tal afecto y las fotografías se almacenaron en una computadora, conformando una base de datos. Para el género femenino, el tipo labial II (líneas bifurcadas), se halló en el 100% de la muestra. En el género masculino los tipos labiales predominantes fueron el I (líneas verticales completas) y II (líneas bifurcadas), ambos presentes en 48 participantes (96%). No existe diferencia significativa entre la frecuencia de tipos de huellas labiales en ambos géneros, por lo que sería factible la identificación humana en Argentina, desde el punto de vista poblacional e individual, en personas con similares características que la muestra estudiada (AU)

A rapid and useful method for gender identification of grey red-backed voles / 中国比较医学杂志

Objective Grey red-backed voles (Myodes rufocanus) are agile, fierce and hard to catch, thus, it is difficult to judge their gender by external appearance, especially for the juvenile voles. Therefore, it may cause difficulties to their allocation and later breeding in laboratories. The aim of this paper is to establish a rapid, simple and accurate method for gender identification of grey red-backed voles. Methods Fresh hair follicles were taken from 6 adult male voles, 3 adult females and 14 4-week-old juvenile voles, 5 male and 5 female 9-week-old Wistar rats, and 5 male and 3 female 6-week-old BALB/c mice. The genomic DNA was extracted using Chelex-100 resin and the zinc-finger Y/X gene (ZFY/ZFX) and the gene of sex-determining region of the Y (SRY) chromosome were amplified by PCR, and a double PCR amplification method was established. Results The ZFY/ZFX gene and SRY gene were simultaneously amplified from the male voles, while only the ZFY/ZFX gene was amplified from the females. The gender of all 23 voles, 10 Wistar rats and 8 BALB/c mice were correctly identified with this method, and the PCR results were consistent with the phenotypic and autopsy results. Conclusions Using fresh hair follicles as experimental materials for gender identification of grey redbacked voles can alleviate shock and damage to the animals. The established double PCR amplification method is accurate, simple, rapid, and deserves to be used for gender identification of grey red-backed voles.

Preparation of colloidal gold test strip against human SMCY antigen / 中国法医学杂志

Objective To establish a rapid method for the detection of SMCY antigen. Methods To Use the technology of colloidal gold immunochromatography with double antibody sandwich assay, the gold labeled pad was coated with colloidal gold labeled SMCY rabbit polyclonal antibody, colloidal gold strip was made for detection of serum, which include 12 serum samples of pig, cattle, dog, chicken and mice and 50 serum samples of human. Results The colloidal gold test strips showed obvious specificity in human and common animal sera and could distinguish between male and female sera. Conclusion This method can be used to identify the serum of women and men, and has certain species specificity, which provides a direction for forensic science to rapidly identify gender of human samples.

Research Progress on Gene Alterations of Amelogenin Locus in Gender Identification / 法医学杂志

There are two kinds ofamelogeningene mutation, including mutation in primer-binding re-gion ofamelogeningene and micro deletion of Y chromosome encompassingamelogeningene, and the latter is more common. The mechanisms of mutation in primer-binding region ofamelogeningene is nu-cleotide point mutation and the mechanism of micro deletion of Y chromosome encompassingamelo-geningene maybe non-allelic homologous recombination or non-homologous end-joining. Among the population worldwide, there is a notably higher frequency ofamelogeningene mutations in Indian popu-lation, Sri Lanka population and Nepalese population which reside within the Indian subcontinent. Thoughamelogeningene mutations have little impact on fertility and phenotype, they might cause incor-rect result in gender identification. Using composite-amplification kit which including autosomal STR lo-cus,amelogeningene locus and multiple Y-STR locus, could avoid wrong gender identification caused byamelogeningene mutation.

Research Progress on Gene Alterations of Amelogenin Locus in Gender Identification / 法医学杂志

There are two kinds of amelogenin gene mutation, including mutation in primer-binding region of amelogenin gene and micro deletion of Y chromosome encompassing amelogenin gene, and the latter is more common. The mechanisms of mutation in primer-binding region of amelogenin gene is nucleotide point mutation and the mechanism of micro deletion of Y chromosome encompassing amelogenin gene maybe non-allelic homologous recombination or non-homologous end-joining. Among the population worldwide, there is a notably higher frequency of amelogenin gene mutations in Indian population, Sri Lanka population and Nepalese population which reside within the Indian subcontinent. Though amelogenin gene mutations have little impact on fertility and phenotype, they might cause incorrect result in gender identification. Using composite-amplification kit which including autosomal STR locus, amelogenin gene locus and multiple Y-STR locus, could avoid wrong gender identification caused by amelogenin gene mutation.

Identification of Fetal Gender Using Maternal Plasma DNA / 대한산부인과학회잡지

OBJECTIVE: To identify fetal gender using fetal DNA in maternal plasma. METHODS: DNA from maternal plasma of 55 pregnant women(47: inpatients, 8: outpatients) underwent a sensitive Y-PCR assay to identify gender. RESULTS: Of the inpatients, fetus-derived Y sequences were detected in 26(80.6%) of the 31 maternal plasma samples from women bearing male fetuses. None of the 16 women bearing female fetuses had positive results from plasma DNA. Eighteen weeks is earliest gestation of gender identification. Of the outpatients(GA 8-11 weeks), fetus-derived Y sequences were detected in 7 of the 8 maternal plasma. Only one patient's fetal gender(GA 9 weeks) was identified. The others were not identified at this moment. CONCLUSION: We identified fetal DNA in maternal plasma. The sensitivity of Y-PCR was 80.6% in women bearing male fetus and the specificity was 100% in women bearing female fetus.