ABSTRACT
@#Objective:To investigate the specific killing effect of magnetic nanoparticles Fe3O4@PEI induced targeted suicide gene therapycombinedwithmagneticfluidhyperthermiaonhepatomaxenograft.Methods:Thesuicidegenetargetinghepatomap[HRE]AFP-HSVTK and tumor cell imaging reporter gene vector p[HRE]AFP-Luc were constructed by sub-cloning gene recombination method, and tested by restriction endonuclease gel electrophoresis. Fe3O4 nano-particles were prepared by co-precipitation method and modified by Polyethyleneimine (PEI) to obtain the magnetic nano-particles Fe3O4@PEI,which could be used as carrier for tumor gene therapy and a medium for magnetic fluid hyperthermia treatment; and the characterization of Fe3O4@PEI was identified by transmission electron microscopy, particle size analyzer and Fourier transform infrared spectroscopy. The reporter genes p[HRE]AFP-Luc were delivered into the nude mice bearing xenografts via tail vein by Fe3O4@PEI, then the bioluminescence signals of mice were observed in an IVIS system.After the treatment of p[HRE]AFP-HSVTK/Fe3O4@PEI, the tumor cell inhibition rate was examined by MTT assay, the cell apoptosis was tested by Flow cytometry, the in vivo tumor development rate and tumor inhibition rate was tested by animal experiment, and the sub-cellular construction of tumor cells was observed by Transmission electron microscopy. Results: Nano-particles Fe3O4@PEI and recombinant vectors p[HRE]AFP-HSVTK and p[HRE]AFP-Luc were successfully constructed; after tale vein injection, image signals were detected only in tumor tissues via IVIS system, but no obvious pathologic damage in other major organs. In the in vitro cell killing test, the cell proliferation inhibition rate and the cell apoptosis rate in combination group was higher than that in hyperthermia treatment group and gene treatment group [inhibition rate: (76.02±7.33)% vs (42.31±4.28)%， (47.76±4.81)%, all P<0.05; apoptosis rate: (34.05±3.41)% vs (14.41±1.55)%， (11.64±1.20)%， all P<0.01]. The in vivo treatment showed that tumor volume development significantly slowed-down and even decreased in combination treatment group, and the tumor mass were significantly smaller than those of the single treatment groups (all P<0.05); and the tumor cell sub- cellular structure showed obvious apoptotic morphology. Conclusion: the suicide gene p [HRE]AFP-HSVTK has specifickillingeffecton hepatomacells,Fe3O4@PEI can be used as effectivegene treatmentcarrierand mediaof magnetic heperthermia treatment; Fe3O4@PEI mediated target treatment combined with magnetic fluid hyperthermia treatment could specificallyinhibitthehepatomaxenograft.
ABSTRACT
Retinoblastoma(RB) is the most common intraocular malignancy in children.The tumor can originate in one or both eyes.About 40％ of children with RB have inherited form of the disease.The treatment options available for RB include chemoreduction therapy,intra-arterial chemotherapy,radiotherapy,enucleation,genetherapy and focal therapies such as cryotherapy,laser photocoagulation,transpupillary thermotherapy.Currently,with the progress of early diagnosis and treatment,chemotherapy combined focal therapies play an important role in the treatment,which not only improve the survival rates of RB children,but also improve the children's life qualities with salvaging the eve and vision.
ABSTRACT
There has been increasing evidence that transcription of non-coding genome sequence is important to life:Relative to the protein coding sequence and a variety of small molecule RNA.IncRNA research is still only in its infancy.Its function and regulation need to be of further studied.In this paper,the current relationship between the tumor and the IncRNA is reviewed.IncRNA may provide new basis and targets for cancer diagnosis and treatment.
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Objective:To explore antitumor effects of combined interleukin 2(IL 2)gene and tumor necrosis factor(TNF ?)gene therapy in nude mice model with hepatocellular carcinoma.Methods:Nude mice model with hepatocellualr carcinoma was established with BelH7402 cell line.Plasmid vector was used to transfect BelH7402 and nude mice model with the gene for human IL 2 or TNF ? alone or in combination by lipofectamine reagent and tumor size in nude mice model with BelH7402 carcinoma was measured before and after treatment to evaluate the response of the different treatment and control groups.Results:Tumor and BelH7402 transfected with IL 2 or TNF ? gene by liposome mediated were successful.Growth rates of BelH7402 transfected with IL 2 or TNF ? gene were not significantly changed,growth rate of tumor in nude mice model was significantly inhibited in combined IL 2 and TNF ? genetherapy group as compared with the other groups(P
ABSTRACT
Gaucher disease is a commonly inherited, autosomtic recessive lysosomal storage disorder, due to the mutation of structure gene encoding beta-glucocerebrosidase (GC) resulted in the deficiency of the enzyme. Besides enzyme replacement, there is no good ways to treat it. This review briefly introduced the phenotypes, pathologic physiology and general therapy to the disease, strongly stress the genetherapy. From animals to human, from in vitro to in vivo, the review detaily descibe the rapid progress made in genetherapy of Gaucher disease.
ABSTRACT
Inthe past few years, the study on gene therapy has achieved significant progress. However, many issues concerning ethics, safety and techniques inadequacy still exist in gene therapy and need to be solved. This article gives a brief introduction about the history, progress and presently existing problems of gene therapy. Meanwhile, it has put forward the counter measures for solving the existing problems.