Current researches in gene therapy for optic nerve injury / 中华实验眼科杂志

Optic nerve is a part of the central nerve system,therefore,it can not regenerate after injury.In recent years,more attentions have been focused on optic nerve regeneration research.The primary pathological basis of optic nerve injury is loss of retinal ganglion cells (RGCs) and irreversible visual dysfunction.A certain number of RGCs survival and/or rescue allow the recovery of visual function.So gene therapy for optic nerve regeneration becomes a hotspot in recent years.Genetic therapy alters the expression of DNA or RNA in the target cells and thus achieves the purpose of treating disease.Whether genetic therapeutic is successful or not depends on the characteristics of gene vectors,tissue microenvironment and transfection technology.This review summarized recent studies and aimed at a better understanding to gene therapy vectors and methods in rescuing optic nerve injury.

Viral vectors for vaccine applications

Traditional approach of inactivated or live-attenuated vaccine immunization has resulted in impressive success in the reduction and control of infectious disease outbreaks. However, many pathogens remain less amenable to deal with the traditional vaccine strategies, and more appropriate vaccine strategy is in need. Recent discoveries that led to increased understanding of viral molecular biology and genetics has rendered the used of viruses as vaccine platforms and as potential anti-cancer agents. Due to their ability to effectively induce both humoral and cell-mediated immune responses, viral vectors are deemed as an attractive alternative to the traditional platforms to deliver vaccine antigens as well as to specifically target and kill tumor cells. With potential targets ranging from cancers to a vast number of infectious diseases, the benefits resulting from successful application of viral vectors to prevent and treat human diseases can be immense.

Construction and Identification of CD147 siRNA Expression Vector / 中华皮肤科杂志

Objective To construct CD147 siRNA expression vector in o rder to analyze the role of CD147 in the invasion and metastasis of tumors deriv ed from skin.Methods According to CD147 cDNA sequence in the Genebank,a pair of 64-nt oligonucleotides,each containing the sites of restriction endonucleas e at both ends,were designed and synthesized.Oligonucleotides were annealed an d ligated with linearized pSUPER by T4DNA ligase.The recombinants (named pSUPER /CD147 siRNA ) were finally sequenced and identified by PCR and restriction end onuclease digestion.Results CD147 siRNA expression vector was successfully co nstructed and identified by double endonuclease digestion.Sequence analysis of inserted fragment revealed the same sequence as synthesized siRNA oligonucleotid es.Conclusions CD147 siRNA expression vector has been successfully constructed,which paves the way for studying the role of CD147 in the invasion and metasta sis of tumor cells derived from skin as well as in tumor therapy.