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@#Abstract: objective 2+- Toinvestigatethecorrelationbetween geneticpolymorphismofplasmamembraneCa ATPaseisoform2 PMCA2 - Methods ( )andsusceptibilitytonoise inducedhearingloss(NIHL). Atotalof228workerswithNIHLwereselectedas the case group by simple random sampling method,and 230 normal hearing workers with similar age,length and level of noise exposure were selected as the control group.DNA was extracted from peripheral blood of workers of the two groups,and single PMCA2 Results nucleotidepolymorphism (SNP)of wasgenotypedbyMassArraysystem. Theallelefrequenciesofrs1719571 PMCA2 - P and rs14154 of gene in the control population were consistent with Hardy Weinberg equilibrium (both >0.05). There was no significant difference in the distribution of rs1719571 and rs14154 genotypes and allele frequencies between the two P groups(all >0.05).LogisticregressionanalysisshowedthattheriskofNIHLinGAgenotypeofrs1719571waslowerthanthat-P in GG genotype (odds ratio=0.53, 95% confidence interval=0.31 0.90, <0.05), after excluding the effects of age, length of exposure to noise, intensity of exposure to noise, smoking and alcohol consumption. The genotype of SNP rs14154 might not P Conclusion PMCA2 contribute to the genetic susceptibility of NIHL( >0.05). The SNP of rs1719571 is associated with the susceptibilityofNIHL,andGAgenotypemaybeapotentialprotectivefactorforNIHL.
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OBJECTIVE: To investigate the correlation between CYP2C8 gene polymorphisms and the adverse reactions of paclitaxelin in cancer patients.METHODS: Fifty-two patients who received paclitaxel chemotherapy from January 2016 to May 2018 were selected as experimental subjects. The CYP2C8 genotypes of the selected patients were tested and the adverse reactions of paclitaxel were observed, collected and recorded to explore the relationship between adverse reactions and genetic polymorphism.RESULTS: In this study, 25 gene loci were detected, only 7 gene loci had mutation, and the remaining 18 gene loci were all wild-type.In the polymorphism of CYP2C8 gene,the incidence of thrombocytopenia and pain in patients with CYP2C8*1B(-271C>A) wild-type (CC) was significantly higher than that in mutant (CA+AA) (PT) wild-type gene (CC) was significantly lower than that in mutant gene (CT+TT) (P<0.05).CONCLUSION: The polymorphism of CYP2C8 gene may be associated with the adverse reaction of paclitaxel.
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[ABSTRACT]AIM:Tostudytherelationshipbetweenhuman8-oxoguanineglycosylase1(hOGG1)gene Ser326Cys polymorphism and severity of coronary artery lesions in the patients with diabetes mellitus .METHODS: We retrospectively analyzed 323 patients with diabetic mellitus receiving coronary angiography .These patients underwent the test of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), and were divided into Cys/Cys genotype (n=85), Ser/Ser genotype (n=121) and Ser/Cys genotype (n=117) according to the results of PCR-RFLP. All clinical data including history of diseases , complications and biochemical markers , such as blood glucose , blood lipids and so on, were recorded.hOGG1 mRNA and 8-hydroxydeoxyguanosine (8-OHdG) were measured by RT-PCR and ELISA, respectively.The results of coronary angiography such as number and severity of coronary artery with lesions were analyzed by 2 cardiovascular physicians .Gensini score and SYNTAX score were also detected by the unitary criteria .RE-SULTS:(1)8-OHdG in Cys/Cys genotype was higher than that in Ser /Ser genotype and Ser/Cys genotype (P0.05).(2)hOGG1 mRNA ex-pression in Cys/Cys genotype was lower than that in Ser/Ser genotype and Ser/Cys genotype (P0.05) was observed.(3)The probability of triple vessel lesions in Cys/Cys genotype was high and the probability of single vessel lesions in Ser /Cys genotype was low , but the difference among 3 genotypes was not statistically significant (P>0.05).(4)Gensini score and SYNTAX score in Cys/Cys genotype were 48.7 ±15.3 and 39.5 ±17.2, respectively, and the ratio of complex lesions in Cys/Cys genotype was 73.0%.These scores and the ratio of complex lesions were higher than the other 2 genotypes (P0.05).CONCLUSION:hOGG1 gene Ser326Cys polymorphism has relationship with coronary artery lesions in the patients with diabetes mellitus and Cys /Cys genotype might have some impacts on the severity of lesions .The mRNA expression of hOGG1 in the patients with diabetes mellitus carrying Cys/Cys genotype is lower than that in the patients carrying the other 2 genotypes , then decreases the abil-ity of identification and removal of 8-OHdG and the capacity of repairing DNA oxidative damage , and accelerates the devel-opment of atherosclerosis .
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Objective:To study the relationship between genetic polymorphisms of GSTM1 GSTT1 and the susceptibility of laryngeal and hypopharyngeal carcinomas(LHC).Method:The GSTM1 an GSTT1 genotypes were determined by multiplex PCR analysis in 76 LHC patients and 76 population controls.The association be tween the genotypes and LHC risk was measured by odds ratios(ORs)and 95% confidence intervals(95%Cls).Resuit:The frequency of GSTM1 null genotype was 59.2% in the LHC patients and 42.1% in controls(OR=1.935,95%CI=1.069-3.510),the difference was significant(P＜0.01).The frequency of GSTT1 null genotype was 57.9% in the LHC patients and 51.3% in controls.The difference was not significant(P＞0.05).In smokers,the risk of the LHC increased in subjects of GSTM1 null genotype(OR=5.545,95%CI=2.158-13.528).Conclusion:GSTM1 polymorphisms are associated with susceptibility to the LHC.It has the synergistic effects with smoking in the development of the LHC.GSTT1 genotypes might have no association with risk of the LHC in urban Linyi.