ABSTRACT
Introduction: genital chlamydia, which is caused by diverse Chlamydia trachomatis genotypes, is largely asymptomatic. We aimed to identify C. trachomatis genotypes causing genital chlamydia among female sex workers attending a sex workers outreach program clinic in Nairobi, Kenya. Methods: this cross-sectional study was conducted between 18 April 2017 and 19 March 2021. Genitourinary complaints from eligible female sex workers were documented using a structured questionnaire. Endocervical swabs were collected for laboratory analysis. C. trachomatis plasmid DNA was extracted, PCR-amplified, and sequenced. Consensus sequences were generated and aligned with reference sequences to determine the C.trachomatis genotypes. Bivariate analysis was used to determine the association between genitourinary complaints and genital chlamydia. Results: endocervical swabs were collected from a total of 348 participants. Of these, 46 (13.2%) were positive for C. trachomatis. Most (297, 85.3%) of the participants presented with pelvic discharge with or without other symptoms. Fifteen (15, 4.3%) had abdominal pain and 3 (0.9%) had an itchy vulva. There was no statistically significant relationship between clinical presentation and genital chlamydia. Twenty-three samples were successfully sequenced. Each sequence was at least 90% identical to each of the 13 references C. trachomatis genotypes A, B, C, D, E, F, G, Ia, J, L1, L2, L2b and L3. Conclusion: we found no significant association between individual genitourinary complaints and genital chlamydia infection. The C. trachomatis genotypes circulating amongst female sex workers in Nairobi could be related to genotypes A, B, C, D, E, F, G, Ia, J, L1, L2, L2b, and L3.
Subject(s)
Humans , FemaleABSTRACT
Objective: The major objective of this study was to carry out concurrent testing of cervical swab samples with both conventional and fluorescent staining method using Giemsa stain & direct fluorescent antibody stain (DFA) respectively. The study would enable us to establish an appropriate, effective and sensitive method of diagnosis of genital chlamydial infections within the present set-up.Material & methods: The study “A Comparative Study of laboratory Diagnosis of Genital Chlamydial Infections in Women by Immunofluorescence and Conventional Staining Method” was conducted on 50 patients attending the OPD of a tertiary care hospital of Ahmedabad. Results: Out of the total 50 cases tested, four samples were found positive containing dark-purple inclusion bodies of Chlamydia trachomatis surrounding the nuclei of the host cells in Giemsa and the same were positive for elementary bodies of C. trachomatis by the DFA staining. Based on these samples, the prevalence of is 8% only.Conclusion: The present comparative study of Giemsa and DFA staining for Chlamydia trachomatis infections in females showed that both methods are equally sensitive for the detection of the microorganism.