ABSTRACT
Objective@#To investigate the neuroprotective effect of hyaluronidase(HAase) on rabbit brain tissue in germinal matrix-intraventricular hemorrhage(GM-IVH) premature rabbits.@*Methods@#Eighty premature rabbits of gestation age 29 days were randomly divided into normal group, GM-IVH control group and HAase treatment group.The rabbits in GM-IVH control group and HAase treatment group were given intraperitoneal injection of 50 g/L glycerol to establish GM-IVH animal model, while the premature rabbits in normal group were given the same dose of 9 g/L saline.The GM-IVH was screened by using cranial ultrasound.The premature rabbits in HAase treatment group were given HAase into the lateral forebrain while the rabbits in the GM-IVH control group were given the same dose of 9 g/L saline.In 3 days, 7 days and 14 days after birth, the premature rabbits were killed and brain tissue were separated.The expression of neuron-glial antigen 2(NG2) were detected by adopting immunohistochemical method.The expression of myelin basic protein(MBP) were detected by Western blot method.@*Results@#The expression of NG2 in 3 groups of premature rabbits decreased gradually with the increase of age[the values of NG2 in the normal group were(62.65±33.58)×104, (15.61±4.22)×104, and (13.54±4.51)×104 on the 3rd, 7th, and 14th day; those in the GM-IVH control group were (54.58±25.48)×104, (48.91±22.49)×104, (7.18±2.28)×104 on the 3rd, 7th, and 14th day; those in the HAase treatment group were (148.13±27.30)×104, (88.38±14.55)×104, (77.98±18.96)×104 on the 3rd, 7th, and 14th day]. At the same time, the expression of NG2 in the HAase treatment group was higher than that of the GM-IVH control group, and the differences were statistically significant among the 3 groups at any time(all P<0.05). The expression of MBP protein in the 3 groups increased with the increase of day age[MBP protein in the normal group were (0.30±0.22)×103, (1.91±0.43)×103, and (5.67±2.14) ×103 on the 3rd, 7th, and 14th day; those in the GM-IVH control group were (0.87±0.12)×103, (1.15±0.22)×103 and (2.54±0.69) ×103 on the 3rd, 7th, and 14th day; those in the HAase treatment group were (0.91±1.01)×103, (2.25±0.66)×103, (3.40±1.10)×103 on the 3rd, 7th, and 14th day]. The difference was statistically significant between the normal group and the HAase treatment group(P<0.05).@*Conclusions@#After HAase treatment, the expression of NG2 and MBP can be improved to a certain extent, promoting the development of oligodendrocytes and regeneration of myelin sheath.
ABSTRACT
Objective To investigate the neuroprotective effect of hyaluronidase( HAase)on rabbit brain tissue in germinal matrix-intraventricular hemorrhage( GM -IVH)premature rabbits. Methods Eighty premature rabbits of gestation age 29 days were randomly divided into normal group,GM-IVH control group and HAase treatment group. The rabbits in GM -IVH control group and HAase treatment group were given intraperitoneal injection of 50 g/L glycerol to establish GM-IVH animal model,while the premature rabbits in normal group were given the same dose of 9 g/L saline. The GM-IVH was screened by using cranial ultrasound. The premature rabbits in HAase treat-ment group were given HAase into the lateral forebrain while the rabbits in the GM-IVH control group were given the same dose of 9 g/L saline. In 3 days,7 days and 14 days after birth,the premature rabbits were killed and brain tissue were separated. The expression of neuron - glial antigen 2( NG2 )were detected by adopting immunohistochemical method. The expression of myelin basic protei(n MBP)were detected by Western blot method. Results The expression of NG2 in 3 groups of premature rabbits decreased gradually with the increase of ag[e the values of NG2 in the normal group were(62. 65 ± 33. 58)×104(,15. 61 ± 4. 22)×104 ,and(13. 54 ± 4. 51)×104 on the 3rd ,7th ,and 14th day;those in the GM-IVH control group were(54. 58 ± 25. 48)×104 ,(48. 91 ± 22. 49)×104 ,(7. 18 ± 2. 28)×104 on the 3rd ,7th , and 14th day;those in the HAase treatment group were(148. 13 ± 27. 30)×104 ,(88. 38 ± 14. 55)×104 ,(77. 98 ± 18. 96)×104 on the 3rd,7th,and 14th day]. At the same time,the expression of NG2 in the HAase treatment group was higher than that of the GM-IVH control group,and the differences were statistically significant among the 3 groups at any time(all P<0. 05). The expression of MBP protein in the 3 groups increased with the increase of day age[MBP protein in the normal group were(0. 30 ± 0. 22)×103 ,(1. 91 ± 0. 43)×103 ,and(5. 67 ± 2. 14)×103 on the 3rd , 7th,and 14th day;those in the GM-IVH control group were(0. 87 ± 0. 12)×103,(1. 15 ± 0. 22)×103 and(2. 54 ± 0. 69)×103 on the 3rd,7th,and 14th day;those in the HAase treatment group were(0. 91 ± 1. 01)×103 ,(2. 25 ± 0. 66)×103 ,(3. 40 ± 1. 10)×103 on the 3rd,7th ,and 14th day]. The difference was statistically significant between the normal group and the HAase treatment grou(p P<0. 05). Conclusions After HAase treatment,the expression of NG2 and MBP can be improved to a certain extent,promoting the development of oligodendrocytes and regeneration of myelin sheath.
ABSTRACT
OBJECTIVE: The purpose of this study is to identify the risk factors associated with the development of germinal matrix-intraventricular hemorrhage (GM-IVH) and the relationship of the severity of disease and prematurity. METHODS: A total of 168 premature neonates whose birth weight < or =1500 g or gestational age < or =34 weeks were examined by cranial ultrasound (CUS) for detection of GM-IVH among the babies admitted between January 2011 and December 2012 in our medical center neonatal intensive care unit. The babies were divided into two groups : GM-IVH and non-IVH. Clinical presentations, precipitating factors of the patients and maternal factors were analyzed. RESULTS: In univariate analysis, gestational age, birth weight, delivery method, presence of premature rupture of membrane (PROM) and level of sodium and glucose were statistically meaningful factors (p<0.05). But only two factors, gestational age and presence of patent ductus arteriosus (PDA) were statistically meaningful in multivariate logistic regression (p<0.05). Delivery method [normal vaginal delivery (NVD) to Caeserean section] was borderline significant (p<0.10). CONCLUSION: Presence of PDA and gestational age were the important risk factors associated with development of GM-IVH.
Subject(s)
Humans , Infant, Newborn , Birth Weight , Ductus Arteriosus, Patent , Gestational Age , Glucose , Hemorrhage , Intensive Care, Neonatal , Logistic Models , Membranes , Precipitating Factors , Risk Factors , Rupture , Sodium , UltrasonographyABSTRACT
Of all types of intracranial hemorrhage (ICH) in the neonates, germinal matrix intraventricular hemorrhage(GM-IVH) in the premature infant is the most common type, mainly attributed to the existence of immature germinal matrix, lt's usually lack of visible and specific symptoms and signs in the nervous system, so its early and final diagnosis depends on the imaging examine. GM-IVH can cause periventricular hemorrhagic infarction, post-hemorrhagic hydrocephalus, periventricular leukomalacia and the associated cerebellar hemorrhagic injury, which are critical determinants of neonatal morbidity, mortality, and neurodevelopmental outcome in the survivors. The overall aim of this article is to review the current knowledge of the cause,mechanisms, imaging diagnosis, complication, management and outcome of GM-IVH in the preterm infant.