Syndrome of inappropriate antidiuretic hormone secretion in neonates with birth asphyxia at tertiary care centre

Background: Syndrome of inappropriate antidiuretic hormone is an important metabolic complication of perinatal asphyxia. This study aims to study the occurrence of syndrome of inappropriate antidiuretic hormone in different grades of perinatal asphyxia and to find out the correlation of syndrome of inappropriate antidiuretic hormone to gestational age and birth weight in birth asphyxiated babies.Methods: It was a hospital-based cohort study. 50 neonates with different grades of asphyxia were enrolled in the study and 50 controls were taken. Syndrome of inappropriate antidiuretic hormone was diagnosed based on calculated plasma osmolality, serum and urine sodium, urine specific gravity and analyzed with different grades of birth asphyxia and birthweight and gestation age.Results: The occurrence of SIADH in birth asphyxiated babies was 26% and none of the babies in control group developed SIADH. The occurrence was more in the severely asphyxiated babies (53.8%), followed by moderate (30.8%) and then mildly asphyxiated babies (15.4%). Hyponatremia was seen in 26% of birth asphyxiated babies and can be used as a marker of SIADH. In relation to gestation age and birth weight there was no statistically significant correlation between SIADH and different grades of asphyxia. Mortality was high among the neonates who developed SIADH.Conclusions: The occurrence of SIADH was 26% in asphyxiated neonates and high in severe asphyxia and gestation age and birth weight beard no significant co relation.

Maternal and neonatal outcomes in pregnancies complicated by maternal obesity

Background: Maternal obesity imparts elevated maternal and neo natal adverse outcomes. Aim of the study was to evaluate and analyse the maternal and neonatal outcomes in obese mothers.Methods: This was a prospective case control study in a tertiary care high risk referral center. Antenatal women with first trimester Body mass index (BMI) of more than 30 Kg/m2 constituted the cases and those antenatal women with BMI <25 Kg/m2 formed the controls.Results: Mean age was slightly more (28.6±4.3 years vs 26.3±3.6 years: P<0.0001) in the obese group. Obese women were significantly more likely to have  of gestational diabetes (OR 5.2, 95% CI 3.2-8.7 P<0.00001), gestational hypertension (Or 3.5, 95% CI 2.1-5.9 P<0.0001) , induction of labour (OR 2.5, 95% CI 1.8-3.6 P<0.0001), failed induction of labour (OR 2.4, 95% CI 1.3-4.2 P=0.003), Preeclampsia (OR 2.8, 95% CI 1.6-4.9 P=0.0002), Caesarian section (Or 4.0, 95% CI 2.9-5.9 P<0.0001) and Postpartum hemorrhage (OR 4.0,95% CI 1.1-14.3 P=0.034), prolonged hospital stay (OR 12.8, 95% CI 7.7-21.1 P<0.0001)  and adverse neonatal outcomes such as low(<7) Apgar (OR 3.2, 95% CI 1.1-10.0 P=0.03), Large for gestational age babies (OR 3.1, 95% Cl 2.1-4.5 P<0.0001) and transfer to new born nursery (OR 3.4, 95% CI 2.3 -5.2 P<0.0001).Conclusions: Maternal obesity in pregnancy is high risk and has many adverse maternal and neonatal outcomes warranting specialized antenatal, intranatal and post-natal care.

Perinatal monitoring of fetal growth restriction / 中国实用妇科与产科杂志

Based on domestic and international guidelines,clinical researches and expert consensus,it is stated that fetal growth restriction is the fetal low weight,clinically accompanied by abnormal placenta and umbilical cord blood flow.This paper summarized the significance and clinical value of the color Doppler detection during perinatal period,and discussed the measures to reduce the adverse outcome of fetal growth restriction during delivery.

Characteristics and Cardiomyogenic Potential of Rat Fetal Cardiac Progenitor Cells at Different Developmental Stage

In recent years, several kinds of cardiac progenitor cells have been identified and isolated from heart tissue. These cells showed differentiation potential into cardiomyocytes, smooth muscle cells, and endothelial cells in vitro and in vivo. Morphogenetic events are tightly regulated during development to determine cell destiny and reshape the embryonic lineage. In this study, we directly compared the characteristics of rat fetal cardiac progenitor cells (rFCPCs) isolated from the chamber formation stage at embryonic day 12 (E12) and at the septation stage of E15. Both kinds of rFCPCs expressed mesenchymal stem cell markers (CD105, CD73, and CD29) but not CD34 and CD45. The E12 rFCPCs expressed a high level of Oct4 compared to E15 until passage 5 and showed a steep decline of Nkx2.5 expression at passage 5. However, Nkx2.5 expression at E15 was maintained until passage 5 and Oct4 expression slightly increased at passage 5. We also detected an intense staining for Oct4 antibody in E12 heart tissue sections. The average doubling time of the E12 rFCPCs from passage 3 to passage 15 was about 5 hours longer than E15. These cells could also be induced into cardiomyocytes expressing α-MHC, cTnT, cTnC, and Cx43 under cardiomyogenic culture conditions and rFCPCs at E15 showed more intense staining of α-MHC than cells at E12 by immunocytochemistry. Taken together, our results show that developmental differences between E12 and E15 may influence their properties and differentiation. Furthermore those differences should be considered when deciding on the optimal cell source for cell replacement therapy in cardiovascular regeneration.

Ultrasound assessment of the closure time and Z scores of neonatal ductus venosus / 中国新生儿科杂志

Objective To study the closure time of neonatal ductus venosus and the Z score regression equation, and to explore the variation of closure time of neonatal ductus venosus with gestationalage.Methods Color doppler ultrasound was applied to detect the closure time of neonatal ductus venosus in normal newborns, Z score regression equation ( lnY =a +bX +cX2 ) for the closure time of neonatal ductus venosus ( Y ) was developed by regression analysis which used gestational age ( X) as an independent variable. The Z scores of the closure time of neonatal ductus venosus in different gestational age were calculated by the formula [ Z = ( M - Y )/S x , M for observation value, Y for predictivevalue].Results There were 432 cases in our study.The closure time of neonatal ductus venosus was negatively related to gestational age ( r = -0. 938 , P <0. 001 ) . The nonlinear regression equation was lnY= -5. 228+0. 089X-0. 000228X2, R2 =0. 854, Sx =0. 214(P<0. 001). Based on the predicted mean of the closure time and Sx related to different gestational age, Z scores for specific closure time of neonatal ductus venosus could be calculated by the formula [ Z =( M -Y)/Sx ] . The Z scores were normally distributed,and did not change with gestational age.Conclusions Theclosure & nbsp;time of neonatal ductus venosus is negatively related to gestational age. The Z scores obtained by the predicted nonlinear regression equation are normally distributed.

Patrones de peso al nacer en Venezuela: influencia de la edad materna y la paridad / Birth-weight pattern in Venezuela: influence of maternal age and parity

Validar varios patrones de peso al nacer, incluyendo aquellos derivados de estudios nacionales. Estudio analítico, de corte transversal. La validación se basó en una muestra de 16434 neonatos (2000 - 2004), que se clasificaron por edad gestacional según cada patrón. Los análisis se basaron en la distribución percentilar (< 10°, 10 - 90°, >90°), comparando las frecuencias observadas y predichas por la prueba X². Universidad Centroccidental "Lisandro Alvarado" / Hospital Central Universitario "Antonio María Pineda" Las clasificaciones fueron muy similares, aunque fueron ligeramente mejores para los patrones de Battaglia y Lubchenco. Hubo notables diferencias relacionadas son la edad y paridad maternas, cuantitativamente similares a las atribuibles al sexo del neonato. Parece recomendable seguir usando los patrones de Battaglia y Lubchenco para clasificar los neonatos, al menos hasta que un nuevo y mejor grupo nacional de patrones esté disponible

To validate several birth-weight-for-gestation-age charts, including those derived from national studies. Analytic, cross-sectional study. The validation was based on a sample of 16434 newborns (2000 - 2004), which were classified by gestational age and birth according to each chart. Analyses were based on the percentile distribution (< 10°, 10 - 90°, >90°), by comparing the observed and predicted frequencies by the X² test. Universidad Centroccidental "Lisandro Alvarado" / Hospital Central Universitario "Antonio María Pineda". The resulting classifications were very similar, although the categorization seemed slightly better when using Battaglia and Lubchenco charts. There were notable differences related to maternal age and parity, quantitatively similar to those attributable to the gender of the newborn. It seems advisable to maintain the use of Battaglia and Lubchenco charts as the basis for classification of newborns, at least until a new and better set of national charts become available

Study on the influence of maternal physiology, mentality and prenatal health care on children born with low birth weight / 中华流行病学杂志

Objective To explore the role of maternal physiology, mentality and prenatal health care on low birth weight (LBW). Methods A LBW-small for gestation age (SGA)-control group retrospective study was conducted based upon a population of infants born from November 2004 to June 2006. 896 cases involved in this study were entirely under voluntary participation and subject could withdraw their consent at any point. All subject recruitment and enrollment took place at Fujian Provincial Maternal and Children Hospital at the time of delivery. Using χ2 test for single factor analysis and logistic regression for multiple analyses Results There were 15 out of 22 single factors having statistical significances, including 2 maternal psychological, 4 physiological, 6 pathological and 3 prenatal health care factors respectively. When multi-factorial stepwise regression analyses was performed, there were 9 factors for main relative factors of LBW ,including women's height, number of prenatal examination, in-normal non-stress test, umbilical cord around the neck, retardation of the umbilical blood flow, week of gestation when the first examination was performed, premature rupture of membrane, preference on the sex of the infant, abnormal family history etc.were influencing the existence of LBW. Awareness on health information appeared to be a protective factor,suggesting that LBW could be prevented during the pregnant period. Conclusion Multiple factors seemed to be contributing to the incidence of low birth weight.

Isolation,cultivation,purification and proliferation of different gestational age human embryonic neural stem cells / 重庆医科大学学报

Objective:To explore the proliferation regularity of different gestational age human embryonic neural stem cells after them were isolated, cultivated and purified successfully.Methods:3~18 weeks human embryo were obtained under asepsis condition, and divided into 3 groups according to gestation age: A group(3~6w), B group(7~11w), C group(12~18w).The each group of NSCs were isolated and purified after primary culture, and identified by single-cell clone culture and immunocytochemistry.(ICC)To study NSCs growth feature by counting numbers and diameters of neurospheres. Results:The cells could survive and proliferate in vitro which were isolated from 3~6 weeks human embryo pallium and neurocanal or 7~18 weeks human fetus pallium. Identification of the cells showed that they had the typical feature of NSC biology in many aspects,such as growth pattern,morphosis,function feature and surface marker. The numbers and diameters of neurospheres from 3~6w gestation age group are much higher than that from 7~18w gestation age group(P